ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of EVEC in ovarian carcinoma and explore its biological significance.</p><p><b>METHODS</b>The expression of EVEC in 22 specimens of normal ovarian tissues and 63 specimens of ovarian cancers was detected by RT-PCR and Western blotting analysis, respectively.</p><p><b>RESULTS</b>RT-PCR showed that the expression level of EVEC in stage I-II ovarian cancer (0.199 ± 0.014) was significantly higher than that in stage III-IV ovarian cancer (0.155 ± 0.015, P < 0.05), and significantly lower than that in normal ovarian tissues (0.415 ± 0.055, P < 0.05). There was no significant difference between the expression levels of EVEC in primary sites and that in corresponding metastatic sites of ovarian cancer (P > 0.05). Furthermore, the results of Western blot also showed that the protein expression level of EVEC in stage I-II ovarian cancer was also significantly lower than that in normal ovarian tissues (0.179 ± 0.026 vs. 0.543 ± 0.032, P < 0.05), and higher than that in stage III-IV ovarian cancer (0.179 ± 0.026 vs. 0.115 ± 0.023, P < 0.05). The EVEC expression level in the epiploic metastasis of stage I-II ovarian cancer was significantly higher than that of stage III-IV ovarian cancer (0.201 ± 0.028 vs. 0.101 ± 0.037, P < 0.05). The expression of EVEC in ovarian carcinoma had no correlation with age, pathologic classification and histological grade (P > 0.05).</p><p><b>CONCLUSIONS</b>EVEC is closely related with carcinoma metastasis. The expression of EVEC in ovarian cancer and its metastatic sites was remarkably decreased. EVEC may play a negative role in the development and metastasis of ovarian cancer and may be a valuable marker in estimation of the prognosis for patients.</p>
Subject(s)
Adult , Female , Humans , Middle Aged , Carcinoma, Endometrioid , Genetics , Metabolism , Pathology , Cystadenocarcinoma, Mucinous , Genetics , Metabolism , Pathology , Cystadenocarcinoma, Serous , Genetics , Metabolism , Pathology , Extracellular Matrix Proteins , Genetics , Metabolism , Neoplasm Staging , Omentum , Metabolism , Ovarian Neoplasms , Genetics , Metabolism , Pathology , Ovary , Metabolism , Peritoneal Neoplasms , Genetics , Metabolism , RNA, Messenger , MetabolismABSTRACT
OBJECTIVE@#To investigate the effect and mechanism of the selective COX-2 inhibitor NS-398 and carboplatin on the human cervical carcinoma cell line Hela.@*METHODS@#The effect of NS-398, carboplatin, and both on the proliferation of Hela cells was assessed by methyl-thiazolyl tetrazolium (MTT) method, and the apoptosis assay and cell cycle distribution were analyzed by flow cytometry.@*RESULTS@#NS-398, and carboplatin inhibited the growth of Hela cells in a dose and time-dependent manner. When combining carboplatin with NS-398, the combined inhibition rate was increased, which nearly equaled the inhibition rate of the double concentrations of carboplatin. Flow cytometry demonstrated that the cell cycle was redistributed: the G(1)-phase cell fraction was increased while the S-phase cell fraction was significantly decreased after the cells were treated with NS-398 (P0.05) while the apoptotic rate was 9.32%+/-0.02% after the treatment with carboplatin (P<0.05).@*CONCLUSION@#NS-398 can inhibit the growth of Hela cells. The effect of NS-398 on Hela cells may not be related to the apoptosis. NS-398 and carboplatin can bring about synergistic effect in chemotherapy on Hela cells.
Subject(s)
Humans , Apoptosis , Carboplatin , Pharmacology , Cell Proliferation , Cyclooxygenase 2 Inhibitors , Pharmacology , Drug Synergism , HeLa Cells , Nitrobenzenes , Pharmacology , Sulfonamides , PharmacologyABSTRACT
OBJECTIVE@#To investigate the probability of improving radical surgery to resect uterus thoroughly and to decrease various complications after the surgery.@*METHODS@#We compared the clinical effect of reformed radical hysterectomy for 79 patients with the effect of tradition radical hysterectomy for 60 patients. Reformed surgery had the following features: We firstly resected the uterus and then dissected the pelvic lymph node. Urinary bladder gap and rectum gap were opened. After exposing the route of ureter, we excised the uterus artery at the point between the ureter and the uterus artery. Cardinal ligament and uterosacral ligament were cut off by electric knives. The pelvic lymph node was dissected with a titanium pinch.@*RESULTS@#The average operation time and the time of keeping uterine pipe were shortened. Bleeding during the operation was reduced. No complication was observed. All patients were followed up for 11 to 20 months and no patient died. One patient recurred.@*CONCLUSION@#Reformed surgery can resect the uterus, dissect the pelvic lymph node thoroughly, and reduce various complications. The reformed surgery can not only ensure the curative effect, but also benefit patient's recovery and life quality.