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1.
China Journal of Chinese Materia Medica ; (24): 1145-1148, 2007.
Article in Chinese | WPRIM | ID: wpr-235249

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the content of guanosine, total alkaloid and individual yield of Pinellia ternata from different populations in China and evaluate its quality.</p><p><b>METHOD</b>Reversed-phase high-performance liquid chromatography (HPLC) was used to determine the content of guanosine. The content of alkaloid was determined by ultra violet spectrophometry. The results were analyzed by SPSS software.</p><p><b>RESULT</b>The contents of guanosine and total alkaloid in P. ternata were 0.0136% -0.0264% and 0.0155% -0.0652% respectively. Individual yield was 0.5536-2.9740 g. All of the populations could be classified into 3 types through hierarchical cluster analysis.</p><p><b>CONCLUSION</b>There exist significant differences in the content of guanosine, total content of alkaloid and individual yield of P. ternata from different populations. It is suggested that breeding and selection for type II of P. ternata should be strengthened.</p>


Subject(s)
Alkaloids , Breeding , China , Chromatography, High Pressure Liquid , Cluster Analysis , Ecosystem , Guanosine , Pharmacognosy , Economics , Pinellia , Chemistry , Classification , Plants, Medicinal , Chemistry , Classification
2.
China Journal of Chinese Materia Medica ; (24): 23-27, 2005.
Article in Chinese | WPRIM | ID: wpr-276653

ABSTRACT

<p><b>OBJECTIVE</b>To study the relationship between plant growth and accumulation of active ingredients in Salvia miltiorrhiza.</p><p><b>METHOD</b>Transplants of S. miltiorrhiza were sampled at 20 day intervals. At each stage, the growth of seedling and root system was recorded and the contents of tanshinone II (A) and salvia acid were measured.</p><p><b>RESULT AND CONCLUSION</b>This study showed that the rapid growth stage of the root system lags behind that of the seedling system, but the growing period of root system lasts longer. The quantitative change of roots reveals a double "S" curve; two rapid growth stages emerge during 30 - 70 days and 140 - 200 days after the seedlings were transplanted. The content of salvia acid reaches the highest level during 140 - 180 days, whereas the content of tanshinone peaks during 100 - 120 days.</p>


Subject(s)
Agriculture , Methods , Abietanes , Phenanthrenes , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Salvia miltiorrhiza , Chemistry , Seedlings , Chemistry
3.
China Journal of Chinese Materia Medica ; (24): 207-211, 2004.
Article in Chinese | WPRIM | ID: wpr-256391

ABSTRACT

<p><b>OBJECTIVE</b>To confirm the amount of fertilizer and the ration of fertilizer.</p><p><b>METHOD</b>Through the experiment of planting Salvia miltiorrhiza in pot and in field were carried out.</p><p><b>RESULT</b>When N:P = 1:1, The production was 1.8 times more than the contrast group in plotting experiment, and in field the production of the highest fertilizer plot were 2.5 times more than the contrast and the higher and high fertilizer plot is 2.25 and 1.2 times respective were than those in the contrast group.</p><p><b>CONCLUSION</b>The proper ration of nitrogenous fertilizer and phosphorous fertilizer is 1:1. Nitrogen shows negative effects to the accumulation of tanshinon IIA, the more the nitrogen ous fertilizer, The less the content of tanshinon IIA. In contrast, phosphor ous fertilizer shows good effects on the accumulation of tanshinon IIA. Phosphorous fertilizer could alleviate the decline of the content of tanshinon IIA by using nitrogenous fertilizer. The accumulation peak of the tanshinon IIA emerge in the period of 150 d.</p>


Subject(s)
Abietanes , Fertilizers , Nitrogen , Pharmacology , Phenanthrenes , Phosphorus , Pharmacology , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Salvia miltiorrhiza , Chemistry
4.
China Journal of Chinese Materia Medica ; (24): 934-938, 2004.
Article in Chinese | WPRIM | ID: wpr-293671

ABSTRACT

<p><b>OBJECTIVE</b>To provide theoretic warrant and technical reference for Salvia miltiorrhizr standardization planting, by carrying out various systemic studies such as observation of seeds configuration fabric, idiosyncrasy of water absorption and groping germinating conditions.</p><p><b>METHOD</b>In the study of configuration fabric, seeds were observed and taken photos by scanning electronic microscope, and heft method was used for measuring changes of water absorption velocity and dehydration velocity. Seeds germination conditions were probed into under the national test regulations for crop seeds and related prescription from international standards.</p><p><b>RESULT</b>(1) There was a layer of slime about 10-20 microm thickness covering epicarp of Danshen seeds. The slime formed as diamond meshwork (reseau) and the weight of it was 8%-10% of total seeds weight. (2) The speed of water absorption of seeds was extremely rapid. The weight of seeds could increase above 10 times as original while the dehydration velocity was quite low. (3) The optimal temperature for the seeds germination is around 25 degrees C, and the germination rate of the new seeds gained yearly was above 75%, but the rate would decrease sharply as years went by. It was also found that the seeds germination power and exponent of vigor were quite high under the temperature transformation between 23 degrees C, 28 degrees C. Such treatments as pre-cool, PEG treatment and infusing with GA3 could increase the rate of seeds germination capacity obviously.</p>


Subject(s)
Germination , Physiology , Plants, Medicinal , Physiology , Salvia miltiorrhiza , Physiology , Seeds , Physiology , Temperature , Water
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