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Chinese Journal of Otorhinolaryngology Head and Neck Surgery ; (12): 455-459, 2006.
Article in Chinese | WPRIM | ID: wpr-298854

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression and activation of NF-kappaB in middle ear cholesteatoma.</p><p><b>METHODS</b>The protein expression of NF-kappaB p65 in 21 middle ear cholesteatoma tissues and 8 normal external ear canal skin obtained in middle ear surgery were examined by immunohistochemistry; NF-kappaB DNA binding activity in these two kinds of tissues were also detected by electrophoretic motility shift assay (EMSA). The influence of cholesteatoma debris on the NF-kappaB DNA binding activity of HaCat cell were further analyzed.</p><p><b>RESULTS</b>All epithelial cell of cholesteatoma revealed a relatively abundant plasma expression of NF-kappaB p65 protein, among which 12 cases showed nuclear positive expression. In contrast,the normal skin epithelium only revealed a sparse plasma distribution of NF-kappaB protein. The levels of NF-kappaB p65 protein expression in the epithelium of middle ear cholesteatoma tissue and normal skin were 0.168 +/- 0.051, 0.088 +/- 0.019 (t = 4.211, P < 0.01), respectively. The NF-kappaB DNA binding activities of cholesteatoma [(16.5 +/- 10.1)%] were also higher than those in normal skin [(1.38 +/- 1.24)%, t = 3. 600, P = 0.014]. The NF-kappaB DNA binding activity of HaCat cell increased when exposed to cholesteatoma debris in a dose dependent manner.</p><p><b>CONCLUSION</b>NF-kappaB might be an important factor which was involved in the occurrence and development of cholesteatoma.</p>


Subject(s)
Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Young Adult , Biopsy , Case-Control Studies , Cholesteatoma, Middle Ear , Metabolism , Pathology , Electrophoretic Mobility Shift Assay , NF-kappa B , Metabolism
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