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1.
Chinese Journal of Medical Education Research ; (12)2006.
Article in Chinese | WPRIM | ID: wpr-686772

ABSTRACT

This paper investigates the main problems existing in research-type key lab construction in higher schools.Based on the investigation,countermeasures are proposed also.

2.
Journal of Forensic Medicine ; (6): 155-159, 2004.
Article in Chinese | WPRIM | ID: wpr-983037

ABSTRACT

OBJECTIVE@#For the purpose of solving a problems of DNA testing of burned bones.@*METHODS@#We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammonium bromide (CTAB) lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purification using the DNA IQ System.@*RESULTS@#The methods were found to be effective in removing the PCR inhibitors from the burned bone. Then the extracted DNA was successfully genotyped by using the florescence labeling STR multiplex method.@*CONCLUSION@#The results of this research will assist forensic scientists in the identification of DNA from victims whose bodies underwent significant trauma or burning, precluding the utilization of traditional forensic DNA identification techniques.


Subject(s)
Female , Humans , Male , Bone and Bones/chemistry , Burns/metabolism , Cetrimonium Compounds , DNA/isolation & purification , DNA Fingerprinting/methods , Forensic Medicine/methods , Polymerase Chain Reaction/methods , Sensitivity and Specificity , Tandem Repeat Sequences , Time Factors
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