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Chinese Journal of Cardiology ; (12): 45-48, 2005.
Article in Chinese | WPRIM | ID: wpr-243511

ABSTRACT

<p><b>OBJECTIVE</b>To study regulation of Ca(2+)-activated K(+) channels (KCa) of mesenteric artery smooth muscle cell (SMC) from 21 old patients with essential hypertension (EH) by endothelin-1 (ET-1) and prostagl E(1) (PGE(1)).</p><p><b>METHODS</b>Mesenteric artery branch from EH was digested by enzyme. Patch clamp technique was used to pull cell-attached and inside-out patches on mesenteric artery SMC from EH and the normotensive patients respectively. The signal channel open probability (Po), open dwell-time (To) and close dwell-time (Tc), open channel number per patch were recorded. After adding Ca(2+) (10(-8) approximately 10(-6) mol/L), ET-1(2 approximately 8 x 10(9) mol/L) and PGE(1) (10, 20, 40, 100, 200, 400 nmol/L) to cytoplasm respectively. The parameters above were observed again.</p><p><b>RESULTS</b>Compared to that of normotensive patients, the activities of KCa channels of patients with EH was higher. After adding Ca(2+) to cytoplasm,the Po of KCa channels in normotensive patients increased significantly. But it was few changes in EH group. KCa channels has dual reaction to ET-1 in normotensive patients. We have found no statistics difference when ET-1 present on KCa channels of EH cases. Whereas PGE(1) can affect KCa channels current and channels kinetic significantly in side-out patches. The Po of KCa channels increased. The To protracted and the Tc curtailed in EH.</p><p><b>CONCLUSIONS</b>The activities of KCa channels of patients with EH increased significantly. but the sensitive to Ca(2+) decreased. ET-1 were few effect to KCa channels. The PGE(1) can activated KCa channels of patients with EH.</p>


Subject(s)
Aged , Female , Humans , Male , Middle Aged , Alprostadil , Pharmacology , Cells, Cultured , Endothelin-1 , Pharmacology , Hypertension , Metabolism , In Vitro Techniques , Mesenteric Arteries , Cell Biology , Muscle, Smooth , Metabolism , Patch-Clamp Techniques , Potassium Channels, Calcium-Activated , Metabolism
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