Impact of interleukin-6 gene polymorphisms and its interaction with obesity on osteoporosis risk in Chinese postmenopausal women

AIMS@#To investigate the association of four single-nucleotide polymorphisms (SNPs) of the IL-6 gene with osteoporosis (OST) susceptibility.@*METHODS@#PCR restriction fragment length polymorphism (PCR-RFLP) was carried out for SNPs detection. Generalized multifactor dimensionality reduction (GMDR) model and logistic regression model were used to examine the interaction between SNP and obesity on OST.@*RESULTS@#Logistic regression model revealed that G allele of rs1800796 and the T allele of rs2069849 were associated with increased OST risk, compared to those with wild genotype. However, no significant correlations were found when analyzing the association of rs1800795 and rs1554606 with OST risk. GMDR analysis suggested that the interaction model composed of the rs1800796 and obesity was the best model with statistical significance (P value from sign test [P] = 0.012), indicating a potential gene-environment interaction between rs1800796 and obesity. Overall, the two-locus models had a cross-validation consistency of 10/10 and had the testing accuracy of 0.641. We also conducted stratified analysis for rs1800796 genotype and obesity, and found that obese subjects with CG or GG genotype have the highest OST risk, compared to subjects with CC genotype, and normal BMI OR (95% CI) = 2.21 (1.52-3.49), after adjustment for age, smoke, and alcohol consumption status.@*CONCLUSIONS@#Our results suggested that the C allele of rs1800796 and the C allele of rs2069849 of IL-6 gene interaction between rs1800796 and abdominal obesity were all associated with increased OST risk.

Application of gyroscope to acetabular cup positioning in total hip arthroplasty / 中国组织工程研究

BACKGROUND:Good position of acetabular prosthesis during total hip replacement plays a key role in success of the operation. Traditional location tool has poor accuracy, is too complicated, and limits its application. OBJECTIVE:To compare the advantages and disadvantages of gyroscope with the traditional technique in acetabular component orientation in total hip arthroplasty. METHODS:Using lateral approach, physicians with different experiences used traditional technique to locate acetabular cup 100 times (20 times in each person; 40° abduction and 15° anteversion), and then used gyroscope to locate 60 times at the same angle in the same plastic hip model. The error between the planed and measured values was recorded. RESULTS AND CONCLUSION: Compared to the traditional technique, the error of the abduction and anteversion decreased obviously. These indicated that the gyroscope can make the orientation of acetabular component more precise in total hip arthroplasty.