ABSTRACT
Objective: To compare the anticoagulation effect of local anticoagulation with citrate in the hemofiltration for sepsis patients with acute renal injury and observe the safety of anticoagulation to provide the basis for clinical anticoagulation of hemofiltration.Methods: Totally 72 patients with sepsis acute kidney injury in the hospital were randomly divided into the control group and the study group with 36 ones in each according to the single and double number.The control group and the study group was respectively treated with heparin sodium and citric acid for anticoagulation with the treatment course of 7 days.The life of filter tube and filter of the two groups were recorded, and the coagulation grade of filter was assessed.The levels of serum creatinine (Scr), blood urea nitrogen (BUN), pH, serum sodium bicarbonate and bicarbonate were measured before and after the treatment.The activated coagulation time (ACT) was measured before the filtration and in 8 h and 24 h after the filtration.The adverse reactions were observed as well.Results: The ratio of blood clotting level at 0-1 grade in the study group was higher than that in the control group, and the ratio of filter coagulation at 2-3 grade in the study group was lower than that in the control group, and the differences were statistically significant (P0.05).Conclusion: The application of hemofiltration in the treatment of sepsis patients with acute renal injury can significantly improve renal function.And local anticoagulation has better anticoagulant effect in septic acute renal injury patients with hemofiltration, which can prolong the life of filter tube and filter with higher security.
ABSTRACT
Objective: To explore the effect of different selenium sources on the function of humoral immunity and antioxidant capacity of rabbits. Method: Thirty-five rabbits were randomly divided into seven groups and vaccinated with rabbit haemorrhagic disease (RHD) dead vaccine. At the same time, rabbits were injected respectively with sodium selenite (0.1 mg/kg bw and 0.3 mg/kg bw), Kappa-selenocanageenan (0.1 mg/kgbw and 0.3 mg/kg bw), DL-selenomethionine (0.1 mg/kg bw and 0.3 mg/kg bw) and physiological saline as control. Antibody against RHD, activity of GSH-Px and content of MDA in rabbit serum were detected on 0, 10, 20, 30d after inoculation. Results: Sodium selenite (0.1 mg/kg bw), Kappa- selenocanageenan (0.3 mg/kg), and DL-selenomethionine (0.3mg/kg bw) could significantly increase the level of RHD antibody. Sodium selenite (0.3 mg/kg bw) and Kappa-selenocanageenan (0.3mg/kg bw) improved the activity of GSH-Px. All selenium groups could decrease serum MDA, but Kappa-selenocanageenan (0.3 mg/kg bw) showed the best effect. Conclusion: Kappa-selenocanageenan (0.3 mg/kg bw) was better than the lower dosage and other selenium sources in the effects on the function in humoral immunity and antioxidant capacity of rabbits.