Analysis of dental clinic and dental chair distribution in Sichuan / 华西口腔医学杂志

OBJECTIVES@#To thoroughly understand the current dental chair equipment status of dental clinics in Sichuan Province and provide a reference for administrative departments.@*METHODS@#Data were collected from a health administrative department and a regional social development yearbook. The number of existing dental clinics and dental chairs in Sichuan Province was investigated.@*RESULTS@#In Sichuan Province, 7 103 dental clinics were determined to be equipped with 21 760 dental chairs. The Gini coefficients of per capita dental clinics in the province were 0.50, 0.22, and 0.06, and the Gini coefficients of per capita dental chairs were 0.68, 0.31, and 0.15; these coefficients had the same distribution as that reflected by the Lorenz curve. In consideration of geographic distribution, the Theil index for the distribution of dental clinics and dental chairs among cities and states were 0.690 7 and 0.822 3, respectively. The overall Theil index va-lues for the distribution of dental clinics and dental chairs in the province were 0.902 4 and 1.079 4, respectively. The difference in the distribution of dental clinics and dental chairs among cities and states in the province contributed 0.765 4 and 0.761 8 to the total difference, respectively.@*CONCLUSIONS@#The allocation of oral health resources in Sichuan Pro-vince is relatively equitable in terms of population and economic distribution but uneven in geographical distribution.

The changes on expression of IL-1βand IL-13 mRNA in lung tissue and serum after drowning rats / 中国法医学杂志

Objective To observe the change in IL-1β,IL-13mRNA expression in drowning rat lungs and serum,so as to investigate the significance of IL-1β and IL-13 mechanism in the development of drowning.Methods SD rats were randomly divided into control group,drowning group.Then using TaqMan probe method to determine the expression of IL-1β and IL-13 mRNA in Right lower lobe of lung tissue and the serum of right ventricle,which were extracted respectively from each group of rats.Results (1) The lung tissue morphological changes:Typical appearance signs and anatomy of drowning group meet ante-mortem drowning feature.(2) The expression of IL-1β,IL-13 in lung tissue:compared with the control group,the expression of IL-1β and IL-13 were slightly decreased,which has no statistical significance.(3) The expression of IL-1β and IL-13 in serum:compared with the control group,the expression of IL-1β and IL-13 were significant increased,both of which has statistical significance.Conclusion (1)The expression of IL-1β and IL-13 were decreased in lung tissue may be due to drowned rats present compensatory anti-inflammatory response syndrome which causes immune incompetent performance.(2) The expression of IL-1β and IL-13 were significant increased in serum may be relate to drown stress and drowning associated acute lung injury after traumatic stress.

The study on the stability of Housekeeping gene mRNA and the correlation between degradation and PMI in dead rats / 中国法医学杂志

Objective To explore the postmortem stability of nine Housekeeping gene mRNA—β-actin, GAPDH, RPL32, PKG1, SDHA, rRPL13, HPRT, TBP, YWHAZ and the correlation between its relative expression and postmortem interval (PMI) in rat brain tissue and skeletal muscle. Methods 33 healty adult SD rats were randomly divided into 11 groups at 11 postmortem interval(0h, 1h, 3h, 6h, 12h, 24h, 2d, 4d, 8d, 16d, 24d), sacrificed by cervic al dislocation and it's anterior tibial muscle and brain tissue (about 50mg) were extracted, respectively. The total RNA was extracted from organizations and the nine mRNA were detected by Real-time RT-PCR. Proper internal reference was selected by geNorm software. To evaluate the stability of genes by GeNorm, NormFinder and BestKeeper software. Regression analysis by spss software. Results The expression of HPRT is the most stable in both brain and skeletal muscle, suitable for reference gene. The relative expression levels of SDHA, RPL32 and TBP in brain tissue had a certain linear relationship with PMI. While there was no significant correlation between the relative expression of RNA and PMI in skeletal muscle. Conclusion Brain tissue and skeletal muscle could be suitable materials for extracting RNA in advanced stage PMI. The relative expression levels of SDHA, RPL32 and TBP had a certain linear relationship with PMI, which could be auxiliary indices for the estimation of PMI.

Effects of survivin gene silencing by small interfering RNA on apoptosis of oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To apply the small interfering RNA (siRNA) targeting survivin to inhibit expression of survivin gene in HSC-3 oral squamous cell carcinoma and to increase the apoptosis of HSC-3 cells.</p><p><b>METHODS</b>siRNA was synthesized and transfected into oral squamous cell carcinoma HSC-3 cells. Compared with negative control group and blank control group, semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR) was used to detect survivin mRNA. Cellular viability was detected by MTT and HSC-3 cell apoptosis was determined by tunnel and flow cytometry.</p><p><b>RESULTS</b>Expression of survivin mRNA was decreased significantly in siRNA group compared with negative control group and blank control group. Cellular viability was not affected in negative control group and blank control group, but cellular viability in siRNA group was significantly decreased. As to the cellular apoptosis rate, the transfected group (24.99% +/- 1.33%) was significantly higher than negative control group (1.24% +/- 0.13%) and blank control group (0.10% +/- 0.02%).</p><p><b>CONCLUSION</b>Survivin gene silenced by siRNA might promote apoptosis of oral squamous cell carcinoma. Survivin siRNA gene therapy would become a new target point for oral squamous cell carcinoma.</p>

The expression of urokinase-type plasminogen activator in oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to reveal the relations between expression and tumor behavior such as invasion, metastasis and prognosis in oral squamous cell carcinoma (SCC) through analyzing the expression of urokinase-type plasminogen activator (UPA).</p><p><b>METHODS</b>With Labelled streptavidin biotin method (LsAB), the expression of UPA was analyzed in 80 cases of oral squamous cell carcinoma, 10 cases of oral normal mucosa and 10 cases of oral leukoplakia.</p><p><b>RESULTS</b>Compared to oral normal mucosa and oral leukoplakia, the expression of UPA was significantly higher in SCC. The expression of UPA in SCC cases with lymph node metastasis was significantly higher than in cases without metastasis. The expression in cases with good prognosis was significantly higher than in those with poor prognosis and the expression was significantly higher in cases with invasive growth than in those with ulcerative and papillary growth.</p><p><b>CONCLUSION</b>The results obtained indicated that high expression of UPA in SCC might be closely related to lymph node metastasis, invasive growth and poor prognosis.</p>

The effect of heat shock on the expression of urokinase-type plasminogen activator in human tongue squamous cell carcinoma cell line (Tca8113) / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to observe the expression of urokinase-type plasminogen activator (UPA) in human tongue squamous cell carcinoma cell line (Tca8113) after heat shock with different dosage.</p><p><b>METHODS</b>The expression of UPA protein of Tca8113 after different heating temperatures(37 degrees C, 40 degrees C, 43 degrees C and 45 degrees C) treatment was examined by immunohistochemical technique (IH) and flow cytometry (FCM).</p><p><b>RESULTS</b>Compared with 37 degrees C group, the UPA protein expression in 40 degrees C and 43 degrees C groups decreased significantly (P < 0.05); however, the UPA protein expression in 45 degrees C group decreased but no statistical difference was found.</p><p><b>CONCLUSION</b>Hyperthermia could inhibit invasion and metastasis of oral squamous cell carcinoma and could be considered as a safe method in curing the tumor.</p>

The effect of hypoxia on uPA activity in human tongue carcinoma cell line Tca8113 / 实用口腔医学杂志

Objective:To observe the activity of urokinase-type plasminogen activator (uPA) in human tongue squamous cell carcinoma cell line Tca8113 under hypoxia in vitro. Methods:According to the different time of hypoxia, the test was divided into four groups as following: (1) 0 h; (2) 6 h;(3)12 h;(4)24 h. The expression of uPA was examined using immunochemistry. The expression of uPA mRNA was examined using in situ hybridization (ISH). The results of ISH staining of uPA mRNA were analysed using Spot and IPP image analysis system.Results:The uPA expression was found in cytoplasm and cytomembrane of Tca8113 cells.uPA mRNA expression was found in cytoplasm of the cells.The staining intensity of uPA mRNA in the groups of (1),(2),(3) and (4) was 0.175 251?(0.013) 863,0.263 191?(0.000 680),0.406 745?0.014 016 and 0.478 919?0.018 998 respectively(P

The effect of heat shock on the expression of urokinase-type plasminogen activator receptor in human tongue squamous cell carcinoma Tca 8113 cells / 实用口腔医学杂志

Objective: To observe the expression of urokinase-type plasminogen activator receptor (uPAR) in human tongue squamous cell carcinoma Tca 8113 cells at different dosages of heat shock. Methods:The expression of uPAR protein in Tca 8113 cells was examined using immunohistochemical technique (IH) and flow cytometry (FCM) after the cells had been treated at 37,40,43 or 45 ℃ for 40 min. Results:The fluorescent intensity of uPAR protein of Tca 8113 cells treated at 37,40,43 and 45 ℃ was 11.36?0.06,9.98?0.12, 10.37?0.05 and 11.31?0.10 respectively.Conclusion:Heat shock can reduce the expression of the uPAR protein of Tca 8113 cells.

Expression of heat shock response protein induced by heat shock in human Tca8113 cells / 实用口腔医学杂志

Objective: To investigate the expression of heat shock response protein(HSP_ 70 ) in human squamous tongue cancer Tca8113 cells during the recovery periods of heat shock.Methods:Tca8113 cells were subjected to heat shock at 43 ℃ for 30 min, then the cells were cultured for 2,4,6,8,12,24 and 48 h respectively. The expression of HSP_ 70 in the cells was examined with immunohistochemical method, Quantitative analysis was performed by FCM and the cell vitality was detected by MTT method.Results:Heat shock induced HSP_ 70 expression in Tca8113 cells at 43 ℃ for 30 min and the maximum proportion of the positive cells were observed and HSP_ 70 reached the maximum value at 12 h after heat shock(P