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Objective:To compare gene expression profiles in normal human cervical epithelial cells (HcerEpic) before and after Chlamydia trachomatis ( Ct) infection. Methods:HcerEpic cells that were pretreated with DEAE-D were infected with Ct serotype E standard strain and then cultured for 44 h. Uninfected HcerEpic cells were used as the control group. Total RNA was extracted from the cells in each group and reverse transcribed to construct a cDNA library. Differences in gene expression profiles between the two groups were analyzed by high-throughput sequencing and the representative genes were selected for verification by qPCR. Results:A total of 23 997 genes were detected, including 125 differentially expressed genes. Among the 125 genes, 119 were up-regulated and six were down-regulated. GO analysis showed that the differentially expressed genes were enriched in several biological processes including defense response to virus, typeⅠinterferon signaling pathway and cellular responses to typeⅠinterferons. KEGG enrichment analysis showed the differentially expressed genes were mainly enriched in the pathways related to virus infections, such as influenza A virus, herpes simplex virus, EB virus and HPV, and NOD-like receptor pathway.Conclusions:There were significant differences in transcriptome profiles of HcerEpic cells before and after Ct infection. The differentially expressed genes were mainly enriched in the interferon pathway, which was closely related to the antiviral processes in cells. qPCR verified the differentially expressed genes and the genes closely related to the interferon pathway, such as ISG15, IFIT2, OASL and UBE2L6.
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Objective To investigate the expressions of Apaf-1 and Caspase-9 protein in ovarian serous carcinoma(OSC) and their clinicopathological significance.Methods The expressions of Apaf-1 and Caspase-9 protein in 45 cases of OSC,60 cases of ovarian serous cystadenomas and 32 cases of ovarian borderline serous cystadenomas were detected by immunohistochemical SP method.The relationship between the expressions of these two proteins and the clinicopathological features of OSC and the correlation between Apaf-1 and Caspase-9 expressions in OSC were analyzed.Results The positive rates of Apaf-1 in OSC,ovarian serous cystadenoma and ovarian borderline serous cystadenoma were 24.4%(11/45),75.0%(45/60) and 46.9%(15/32),the difference was statistically significant (χ2=26.734,P<0.01).Apaf-1 expression was correlated with pathological grade,clinical stage and lymph nodes metastasis (χ2=6.318,7.565,5.554,P<0.05).The expression rates of Caspase-9 in OSC,ovarian serous cystadenoma and ovarian borderline serous cystadenoma were 28.9%(13/45),83.3%(50/60) and 56.3%(18/32),the difference was statistically significant (χ2=31.682,P<0.01).The expression of Caspase-9 was correlated with pathological grade,clinical stage and lymph nodes metastasis (χ2=5.750,4.391,5.466,P<0.05).In OSC,the expressions of Apaf-1 and Capase-9 were positively correlated (k=0.433,P=0.003).Conclusion The low expressions of Apaf-1 and Caspase-9 in OSC may be related to the occurrence and development of OSC.
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Objective To compare the biomechanical properties of non-locking compression plate (DCP) and locking compression plate (LCP) in the internal fixation of humeral shaft fracture by means of finite element analysis.Methods Three-dimensional finite element models were constructed to simulate DCP and LCP internal fixation of humeral transverse fracture.The DCP and LCP groups were compared in terms of peak stress on the humeral fracture fragments (MPa),peak stress on the screws (MPa),and overall displacement peak value (mm) under 4 basic loads (bending,shear,torsion and compression).The biomechanical stability was analyzed after fracture fixation.Results The stress tended to concentrate at the connection sites of plate and screws and distributed evenly on DCP.The stress distributed in a gradient manner at the multiple screw holes and tended to concentrated on the central screws on LCP.Under the bending,shear and torsion loadings,the peak stresses on the fracture fragments and screws in the DCP fixation were larger than in the LCP fixation.However,under the compression loading,the peak stresses on the fracture fragments and screws in the DCP fixation were smaller than in the LCP fixation.DCP and LCP had similar trends in displacement.Under the bending,shear and torsion loadings,the overall displacement peak values in LCP fixation were smaller.However,under the compression loading,the overall displacement peak values in DCP fixation were smaller.Conclusions DCP and LCP have similar biomechanical properties to resist bending,shear,torsion and compression.Due to the gradient distribution of stress among the screw holes,LCP is more suitable for patients with comminuted fracture or osteoporosis.Stress distribution is more even in DCP.Surgeons should consider the advantages of both DCP and LCP to achieve better stability.