ABSTRACT
Objective A new method for serum detection based on surface-enhanced Raman spectroscopy was explored by comparing the Raman spectra between normal mice and influenza virus-infected mice. Methods The nano-silver sol was used as the active substrate.The Raman spectra of the normal group,the model group and the Tamiflu control group were detected by portable Raman spectroscopy,and the partial least squares discrimina-tion analysis(OPLS-DA)was performed by the orthogonal correction. The number of RNA replicas of influenza virus in lung tissue was measured by RT-PCR as a control method. Results At the 3rd or 5th days,the serum of the normal group,the model group and the Tamiflu control group showed a significant trend. By the ROC curve evaluation,the predictive ability of 3 groups of serum SERS models established by OPLS-DA was high,which could distinguish and differentiate 3 groups of serum. Conclusions The results of SERS and RT-PCR detections were consistent.The preliminary results show that SERS pattern can help to identify and diagnose whether the body is infected with influenza virus.
ABSTRACT
Objective To investigate the protective effect and possible mechanism of amentoflavone (AF) on bone marrow cells of mice injured by irradiation. Methods Primary bone marrow cells of male C57BL/6 mice were cultured and randomly divided into 4 groups (normal control, radiation control,AF 2.5μmol/L and 5μmol/L), with 3 samples in each group. After treated with AF for 12 h, the cells were injured by 12 Gy 60Coγirradiation. 6 h and 12 h post-irradiation, apoptosis was evaluated by using Hoechst 33258 stain, cell cycle was determined by flow cytometry while the level of TNF-α was tested by ELISA. Results The cell cycle arrest and apoptosis were not significantly affected by Amentoflavone. Amentoflavone (5 μmol/L)could significantly inhibit the production of TNF-α on cell supernatant of mouse bone marrow cells at 6 h or 12 h after radiation and 2.5 μmol/L Amentoflavone could significantly inhibit the production of TNF-α at 6 h after radiation. Conclusion Taken together, the data suggest that AF may have radioprotection against damage in mice bone marrow by inhibiting the production of TNF-α.
ABSTRACT
The antihypertensive effect of marine sulfated polysaccharide AHD and its underlying mechanisms were studied in renovascular hypertensive rats (RHR) [two-kidney one clip,Goldblatt(2-K 1C)J. AHD were given orally at doses of 12.5, 25,50mg?kg～-1 once a day for five weeks,simultaneously wity the initiation of the establishment of renovascular hypertensive model. Serum nitric oxide(NO) was determined with NO kit,and plasma Angiotensin E (Ang Ⅱ ) and Eneothelin (ET) Were measured by radioimmumoassays. The results indicated that AHD decreased arteial blood pressure in a dose-dependent manner. Moreover, nitric oxide (NO) contents in serum were dose-dependently elevated,accompanied by dramatic reduction in Ang Ⅱ and ET contents. These findings suggested that AHD exerted its hypotensive activity by enhancing tye release of NO,followed by reduction in production of boty ANG Ⅱ and ET in vivo.