ABSTRACT
OBJECTIVE To investigate the inhibitory effect of berberine (BER) on the invasion and migration of human renal carcinoma cells and its potential mechanism. METHODS Using human renal carcinoma OSRC-2 cell as object, alamarBlue assay was adopted to detect the inhibitory effects of 0 (control group), 25, 50, 75, 100, 125, 150, 175 and 200 μmol/L BER on the proliferation of OSRC-2 cell after treatment for 24 h and 48 h. After treated with 0(control group), 50, 100 μmol/L BER for 48 h, the effect of BER on cell cycle was analyzed by flow cytometry. The migration of OSRC-2 cells in 24 h and 36 h was observed by cell scratch test, and the invasion ability of OSRC-2 cells in 24 h was detected by Transwell assay. The protein expression of methyltransferase-like 3 (METTL3) was detected by Western blot after treatment for 48 h, and RNA methylation quantification kit was used to detect the levels of N6-methyladenosine (m6A) in OSRC-2 cells. RESULTS Compared with control group, BER at different concentrations could significantly decrease the survival rate of OSRC-2 cells (P<0.01), and showed a dose-dependent and time-dependent manner. After 48 h of BER treatment at 50, 100 μmol/L, the cell was arrested in G0/G1 phase (P<0.01). Compared with control group, the migration and invasion abilities of cells in 50, 100 μmol/L BER group were significantly decreased (P<0.05 or P<0.01); the protein expression of METTL3 and the level of m6A in RNA were significantly decreased (P<0.01). CONCLUSIONS BER can inhibit level of m6A by down-regulating the expression of METTL3, thereby inhibiting the invasion and metastasis of human renal carcinoma cells.