ABSTRACT
Circulating tumor cells (CTCs)are special kind of tumor cells in the peripheral blood of patient with tumor.Now CTCs detection has been used in the survival time prediction,post-operational recu-rrence detection,individualized treatment and other aspects in the patients with gastric cancer.As the research going,CTCs will provide new help for the comprehensive treatment of gastric cancer.
ABSTRACT
Metastasis is the essential character of biological behaviors in malignancies,which is also the main reason for the death of patients with tumor. So it is very important to research the metastasis for the pre-vention and treatment of carcinomas. Rectal cancer is one of the most common gastrointestinal cancer,and its prognosis is bad. lymphatic metastasis is one important factor in the determination of colon cancer' s progno-sis. More than 50% patients are found lymphatic metastasis when taking operation. The ralationship of lym-phatic metastasis and prognosis is more important than the original tumor's invasiving. So it is meaningful to study the mechanism of tumor metastasis, and to provide a scientific approach to the prediction of tumor me-tastasis, the precise assessment of prognosis and the treatment of tumor metastasis.
ABSTRACT
Objective:To study the effect of RNA interference(RNAi) on PAR1 gene mRNA expression and the invasive petential of human colon cancer cell lines.Methods:Chemically synthesizeddouble stranded RNA(dsRNA) targeting PAR1 was transfect- ed into human colon cancer cell Lovo using SiPORT Lipid.The transfection eficiency was observed under fluorescence confocal microscopy.Expression of PAR1 mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.Cell penetrate matrigel capacity were determined by invitro experiment.Results:PAR1-siRNA effectively in- hibited PAR1 mRNA and protion expression(P