ABSTRACT
Objective:To observe the changes in bone mineral density and microstructure parameters in sclerostin (SOST) gene knockout (SOST -/-) mice after ovariectomy. Methods:Twelve 4-week-old SOST knockout mice were randomly divided into two groups ( n=6): ovariectomized group (SOV), sham operated group (SSO). Twelve wild-type mice were randomly divided into two groups ( n=6): wild-type ovariectomized group (WTO), wild-type sham operated group (WTS). Twelve weeks later, mice were sacrificed and one lumbar vertebra of each mouse was selected for micro-CT analysis. The bone mineral density, trabecular volume fraction, trabecular number and trabecular thickness were observed and compared in the 4 groups. Results:There was no difference in bone mineral density, trabecular volume fraction, trabecular number and trabecular thickness between SOV and SSO groups. Bone mineral density, trabecular volume fraction, trabecular number and trabecular thickness in SOV and SSO groups were significantly higher than those in WTO and WTS groups ( P<0.001). Bone mineral density, trabecular volume fraction and trabecular number in WTO group were significantly lower than those in WTS group ( P=0.017, 0.039, 0.021, respectively). There was no difference in trabecular thickness between WTO and WTS groups. Conclusions:Sclerostin knockout mice showed high bone mass, and ovariectomy did not lead to bone loss and bone microstructure degeneration, which indicates that slerostin is a potential therapeutic target for postmenopausal osteoporosis.
ABSTRACT
Objective To observe the changes in bone mineral density and microstructure parameters in sclerostin (SOST) gene knockout mice treated with glucocorticoid.Methods 12 4-week-old SOST knockout mice were randomly divided into two groups (n =6):methylprednisolone intervention group [SOM group,methylprednisolone 3 mg/(kg· d),subcutaneous injection],placebo group (SOS group,isovolumetric saline subcutaneous injection).12 wild-type mice were randomly divided into two groups (n =6):wild-type placebo group (WTS group,isovolumetric saline subcutaneous injection),wild methylprednisolone intervention group [WTM group,methylprednisolone 3 mg/(kg · d),subcutaneous injection].12 weeks later,mice were sacrificed and one lumbar vertebra of each mouse was selected for microCT analysis.Results There was no difference in bone mineral density (BMD),trabecular volume fraction,trabecular number and trabecular thickness between SOM and SOS groups (P ＞ 0.05).BMD,trabecular volume fraction,trabecular number and trabecular thickness in SOM and SOS groups were significantly higher than those in WTS and WTM groups (P ＜0.05).BMD,trabecular volume fraction,trabecular number and trabecular thickness in WTM group were significantly lower than those in WTS group (P ＜ 0.05).Conclusions Sclerotin gene knockout mice can resist glucocorticoid-induced bone loss and bone microarchitectural deterioeration.The treatment of osteoporosis with SOST/sclerotin as a target will be an effective method in the future.
ABSTRACT
Objective@#To observe the changes in bone mineral density and microstructure parameters in sclerostin (SOST) gene knockout mice treated with glucocorticoid.@*Methods@#12 4-week-old SOST knockout mice were randomly divided into two groups (n=6): methylprednisolone intervention group [SOM group, methylprednisolone 3 mg/(kg·d), subcutaneous injection], placebo group (SOS group, isovolumetric saline subcutaneous injection). 12 wild-type mice were randomly divided into two groups (n=6): wild-type placebo group (WTS group, isovolumetric saline subcutaneous injection), wild methylprednisolone intervention group [WTM group, methylprednisolone 3 mg/(kg·d), subcutaneous injection]. 12 weeks later, mice were sacrificed and one lumbar vertebra of each mouse was selected for micro-CT analysis.@*Results@#There was no difference in bone mineral density (BMD), trabecular volume fraction, trabecular number and trabecular thickness between SOM and SOS groups (P>0.05). BMD, trabecular volume fraction, trabecular number and trabecular thickness in SOM and SOS groups were significantly higher than those in WTS and WTM groups (P<0.05). BMD, trabecular volume fraction, trabecular number and trabecular thickness in WTM group were significantly lower than those in WTS group (P<0.05).@*Conclusions@#Sclerotin gene knockout mice can resist glucocorticoid-induced bone loss and bone microarchitectural deterioeration. The treatment of osteoporosis with SOST/sclerotin as a target will be an effective method in the future.