ABSTRACT
OBJECTIVE: To establish a RP-HPLC method for the determination of sodium lactate in compound sodium lactate and glucose injection and provide reference for revision of method recorded in Chinese Pharmacopoeia. METHODS: The separation was performed on a Shim-pack VP-ODS C18 analytical column (4.6 mm×250 mm, 5 μm), and the mobile phase was formic acid-dicyclo-hexylamine-water (1:1:998, V/V) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm and the column temperature was maintained at 40℃. The loading volume was 20 μL. The RP-HPLC method and the cation exchange resin method adopted by Chinese Pharmacopoeia were both used to determine the content of sodium lactate in compound sodium lactate and glucose injection, and the results were compared. RESULTS: The linear range of the RP-HPLC method was 0.51-6.07 mg·mL-1 (r=0.9999). The average recovery was 99.47% (RSD=0.75%, n=9). CONCLUSION: The established method of RP-HPLC can be used for the determination of sodium lactate in compound sodium lactate and glucose injection.
ABSTRACT
<p><b>OBJECTIVE</b>To study optimum inclusion conditions for volatile oil from three medicinal slices in Xiongdan Xiaoyan capsules.</p><p><b>METHOD</b>Saturated water solution method (agitation method), ultrasonic method and rubbing method were studied and compared. The preparation conditions of ultrasonic method were investigated by the orthogonal test.</p><p><b>RESULT</b>The optimum inclusion conditions of ultrasonic method were established.</p><p><b>CONCLUSION</b>The ultrasonic method can be used for production of inclusion complex in the factory with high inclusion rate and was effectiveness.</p>