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Objective To explore the effect of lncRNA of growth arrest-specific 5 (lncRNA GAS5) on the radiosensitivity of colon cancer cells by targeting miR-223.Methods The expressions of lncRNA GAS5 in a few of colon cancer cell lines were detected by real-time quantitative PCR (qPCR).The cell lines with low expression level of lncRNA GAS5 were selected for subsequent study.The effect of overexpression of lncRNA GAS5 on the radiosensitivity of colon cancer SW480 cells was detected by cell cloning experiments.The target gene miR-223 of lncRNA GAS5 was predicted and validated by the bioinformatics database starBase and dual luciferase reporter assays.qPCR was used to detect the expression of miR-223 in various colon cancer cell lines and the influence of lncRNA GAS5 overexpression on the expression of miR-223 in SW480 cells.Results Compared with normal human colonic epithelial cells (NCM460),the expressions of lncRNA GAS5 in the colon cancer SW480,LOVO,HT-29 and SW620 cell lines were significantly lower(t =15.25,8.69,14.42,11.62,P ＜ 0.05),with the lowest level in SW480 cells.Both overexpression of lncRNA GAS5 and down-regulation of miR-223 significantly increased the radiosensitivity of colon cancer cells by decreasing cell survival fraction (at 8 Gy,lncRNA GAS5,t =13.51,P ＜ 0.05;anti-miR-223,t =14.93,P ＜ 0.05)and promoting apoptosis (lncRNA GAS5,t =8.30,P ＜ 0.05;anti-miR-223,t =7.32,P ＜ 0.05).Bioinformatics analysis showed that the 3'sequence of lncRNA GAS5 contained the binding sites with miR-223.After overexpression or downregulation of lncRNA GAS5,the expression of miR-223 was enhanced or reduced.Conclusions The lncRNA GAS5 promotes the apoptosis of colon cancer cells and inhibits its survival by targeting miR-223 expression,thereby increases the radiosensitivity of colon cancer cells.
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Objective To evaluate the prognosis influencing factors of early non-small cell luny cancer (NSCLC) after radiotherapy.Methods 81 early NSCLC patients received definitive radiotherapy and were eligible.Among these patients,60 were diagnosed as squamous cell carcinoma,16 were adenocarcinoma and 5 were diagnosed through imaging instead of pathology.45 patients received conventional radiotherapy,36 patients received three dimensional conformal radiotherapy (3D-CRT),All of them received a total dose of 50-96 Gy with a median dose of 67.8 Gy. Kaplan-Meier survival curves and Cox regression model analysis were applied to evaluate the survival and prognostic factors. Results The median survival time was 34 months.The 1-,3- and 5-year survival rates (OS) were 88.7 ％,41.9 ％,21.8 ％,respectively.Karnofsky performance status≥80,Clinical stage, diameter≤4 cm and the therapeutic effect were associated with improving overall survival.Cox hazards model showed that Karnofsky performance status≥ 80 and diameter≤4 cm were likely to be independent positive prognostic factors. Conclusion Karnofsky performance status and tumor diamater can be used to evaluate the prognosis of early NSCLC after radiotherapy.
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Objective To study the quality of life and influencing factors in children with epilepsy.Methods The psychology of 96 children with epilepsy and 50 normal children were evaluated with the American quality of life and the affecting factors were analyzed.Results The quality of life of the epilepsy children was remarkably lower than that of normal children.The main presentation was seizure worry,side effects of drugs and difficulty of social intercourse.The age of initial presentation.the course of ease and so on affected quality of life in children with epilepsy(P＜0.05 or P＜0.01).The factors such as depression,family.environment,memory function,drug influence and seizure worry played important roles in decreasing order of importance.Conclusion QOL of children with epilepsy was remarkablely lower than normal children.General comprehensive care should be applied to children with epilepsy.Adequate drug treatment to control the seizure attack was very important.
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Objective To probe the pathogenesis of ventilator associated pneumonia (VAP) and their resistance to antibiotics of children in pediatric intensive care unit (PICU). Methods A clinical trial in 308 VAP episodes of 134 patients in PICU receiving mechanical ventilation was made. The pathogens and their drug resistance were analyzed. Results A total of 498 pathogen strains were isolated by bacterial culture. The major pathogenic bacteria in VAP were the gram-negative bacilli accounting for 416 strains (83.5%); the gram-positive bacteria were 66 strains (13.2%), staphylococcus aureus 35 strains (7.0%); methicillin-resitant staphylococcus aureus 22 strains (4.4%); staphylococcus epidermidis 19 strains (3.8%); methicillin-resitant staphylococcus epidermidis 11 strains (2.2%); all staphylococcus were sensitive to vancomycin; the fungi were 16 strains (3.2%). Among pathogens, the most common pathogens associated with VAP were klebsiella pneumoniae 155 strains (31.1%), aci netobacter baumanii 56 strains (11.2%), pseudomonas aeruginosa 54 strain (10.8%), escherichia coli 39 strains (7.8%) and staphylococcus aureus 35 strains (7.0%). Their multiple drug-resistance to antibiotics was serious. Drug sensitivity tests indicated that the gram-negative bacilli had lower drug-resistance to such antibiotics as imipenem, amikacin, piperacillin/tazobactam, ceftazidime, cefepime, cefoperazone/sulbactam, ciprofloxacin and amoxycillin/clavulanate. Drug sensitivity tests indicated that the gram-positive bacilli bad lower drug-resistance to such antibiotics as vancomycin, cefazolin, rifampicin, cefaperazone/sulbactam and bad higher drug-resistance to penicillin G and amoxycillin/clavulanate. Polymicrobial pathogens were more isolated in late-onset VAP than those in early-onset VAP. Conclusions The major pathogens in VAP are gram-negative bacilli and there are often multiple drug-resistant and fungus infection was common. It was suggested that the adequate initial empiric antimicrobial treatment should base on the surveillance of etiology and resistance.
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Objective To investigate the differences in psychological problems and behaviors between asth-matic and nonasthmatic children,and to evaluate the psychological interventions. Methods Behaviors and psycholog-ical actions were investigated in the 126 children with asthma and 126 healthy children, after psychological interven-tions of 3 and 6 months,the effects were evaluated in 28 asthmatic children. Results Behavioral and psychological problems were more prevalent in asthmatics than nonasthmaties, psychological and behavioral problems were different in asthmatic children of different severity and gender, psychological and behavioral interventions as well as drug ther-apy have beneficial effects on controlling of asthmatic symptoms and improving the pulmonary function, it could cor-rect and cure the bad behavioral and psychological problems and improve the quality of life. Conclusion Asthma contributes to the development of behavioral and psychological problems in children. Psychological and behavioral in-tervention in children with asthma has many positive effects on controlling the symptoms and improving the pul-monary function,it could correct and cure the bad behavioral and psychological problems and improve the quality of life.
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Objective To explore the changes and significance of blood sugar,C-reactive protein(CRP)and blood gas in children with infantile pneumonia associated with systemic inflammatory response syndrome(SIRS).Methods Levels of blood sugar,blood gas and CRP were measured in 72 children with pneumonia associated with SIRS(divided as Group A),at the same time,the measured results in Group A were compared to those in children with pneumonia of non-SIRS(Group B)and normal children(control group).Results There was insignificantly higher than control group(t=3.274,P0.05),the levels of blood sugar and CRP in Group A were all significantly higher than those in Group B(t=9.9498,P