ABSTRACT
Objective To observe the influence of the sperm abnormalities on conventional invitro fertilization therapy outcome. Methods Sperm of 105 infertile husband's semen was analyzed. The patients were divided into three groups according to the sperm morphology observed Using Kruger 's criteria: normal sperm morphology group (sperm normal morphology ≥ 15 % ), mild sperm abnormalities group( sperm normal morphology 10%- 15%), middle sperm abnormalities group (sperm normal morphology 5 % - 10 % ). Fertilization rate, cleavage rate, good quality embryo rate, implantation rate, clinical pregnancy rate and abortion rates were compared to study the influence of the sperm abnormalities on conventional IVF outcome. Results ①Fertilization rate(79.4 % vs 78.3 % ), cleavage rate(104. 6% vs98. 6%), good quality embryo rate(58. 1% vs 53. 9%), implantation rate (31.7% vs 30. 8%), clinical pregnancy rate(48.1% vs 42. 3%)and abortion rates(13.0% vs 18. 2%)were not significantly different between normal sperm morphology group and mild sperm abnormalities group(P>0. 05). ②The fertilization rate (79.4% vs 63.9%), good quality embryo rate(58. 14% vs 48.23%), implantation rate (31.7% vs 16. 7%) and clinical pregnancy rate(48.1%vs24.0%) of the normal sperm morphology group were higher than middle sperm abnormalities group(P<0.05). The abortion rates(13.04% vs 28. 57%) and cleavage rates(104.6% vs 102.9%) were not significantly different(P>0. 05). ③The fertilization rate (78.3% vs 63.9%), good quality embryo rate (53. 9%vs 48. 2%) of the mild sperm morphology group were higher than middle sperm abnormalities group(P <0.05). Implantation rate (30.8% vs 16. 7%) , clinical pregnancy rate(42.3% vs 24.0%) , abortion rates(13. 0% vs 28. 6%) and cleavage rates(104.6% vs 102.9%) were not significantly different (P>0. 05). Conclusions There is a significant influence of middle sperm abnormalities on IVF fertilization rate, good quality embryo rate, implantation rate and clinical pregnancy rate. However, it could not be influenced on mild sperm abnormalities.
ABSTRACT
Objectve To study the effect of anti-swelling and alleviating pain of Huzhangtongluo liniment. Method Mice with xylol-induced auricular swelling : the mice of each group were administered three times per day for 3 days. The inflammed mice were killed and punched a patch on left and right ear in 8 mm diameter, the weight margin of two patch was the rate of swelling. Mice with formalin-induced podalic swelling:the inflammed mice of each group were administered after 4 hours, by dislocating their cervical vetebra. The mice posterior feet were cut at the same place and weighed, the weight margin of left and right foot was the rate of swelling. Rat with granulation induced by cotton embedded under dermis:the rat of each group were administered and then killed after 6 hours, the granulation tissue of rat was separated, dried in oven at temperature of 70 ℃ and weighed, the data of dry weight was calculated and processed by statistics. The pain threshold of mice stimulated by heat:the pain threshold of administered mice was determined at 30, 60, 90 min. The threshold value was calculated as 60 s if the mice had no action such as licking foot, jumping or lifting foot after 60 s. Result Huzhangtongluo liniment can obviously inhibit auricular swelling induced by xylol and podalic swelling induced by formalin as well as granulation induced by cotton embeded under dermis. The preparations can clearly enhance pain-threshold value of mice in hot-plate method. Conclusion Huzhangtongluo liniment has the action of anti-swelling and alleviating pain.