ABSTRACT
Objective To study the relationship between histological activity index and serum of tumor nec-rosis factor-α( TNF-α) ,peripheral blood T cell subsets in patients with chronic hepatitis B.Methods Histopatholog-ical examinations were performed in 237 patients with chronic hepatitis B and 12 controls.The histological activity index( HAI) were analyzed by knodels method.These patients were divided into the four groups according to histologi-cal activity index classification:the control group(n=12),mild group(n=67),moderate group(n=89),and severe group(n=81).The serum levels of TNF-αwere determined by ELISA,and peripheral blood T cell subsets were ana-lyzed by flow cytometry.Results The serum level of TNF-αin the mild group[(29.65 ±10.15)μg/L],moderate group[(38.96 ±7.32)μg/L]and severe group[(47.73 ±6.99)μg/L]were higher than those in the control group [(13.78 ±6.40)μg/L](q=14.38,19.97,24.83,all P<0.05),significant positive correlation lied between the histological activity index classification and the serum level of TNF-α(r=0.708,P<0.05).The rate of CD8+cells in the moderate group[(27.66 ±6.63)μg/L]and the severe group[(28.98 ±5.92)μg/L]were higher than those in the control group[(22.32 ±1.84)μg/L](q=3.84,4.76,all P<0.05),and the ratio of CD4+/CD8+in the moderate group(1.32 ±0.37) and the severe group(1.19 ±0.30) were lower than those in the control group(1.67 ±0.14) (q=4.20,5.72,all P<0.05),but the rate of CD8+cells and the ratio of CD4+/CD8+showed no significance among the mild group,moderate group and severe group.Conclusion TNF-αand disorder of cellular immunity may play an important role in the development and progression of intrahepatic vascular lesion.
ABSTRACT
Objective To study the relationship between vascular obstruction,inflammation and the pseudolobuli formation in liver tissues with chronic hepatitis B.Methods The liver biopsies from 1256 patients with chronic hepatitis B was observated by HE,histochemical,immunohistochemical and the electricity mirror techniqe.Results (1)The intrahepatic vascular inflammation and obstruction was a histopathological characteristics of liver cirrhosis of chronic hepatitis B;(2)The lesions of vascular inflammation caused the vascular obstruction and induced the proliferations of venous and fiber;(3)The blood vessel of hyperplasia revolved round the liver cell into the irregular cell circular(tiny node).The tiny node settled the composition base in the interest of pseudolobuli formation;(4)The blood vessel of hyperplasia once more inflammation,blocking,fiber deposit constituted the fiber brings,pseudolobuli formation.Conclusion The vascular obstruction,inflammation has a closely relationship with the pseudolobuli formation in liver tissues with chronic hepatitis.