ABSTRACT
RNA splicing contributes to a broad spectrum of post-transcriptional gene regulation during normal development, as well as pathological manifestation of heart diseases. However, the functional role and regulation of splicing in heart failure remain poorly understood. RNA binding protein (RBP), a major component of the splicing machinery, is a critical factor in this process. RNA binding motif protein 24 (RBM24) is a tissue-specific RBP which is highly expressed in human and mouse heart. Previous studies demonstrated the functional role of RBM24 in the embryonic heart development. However, the role of RBM24 in postnatal heart development and heart disease has not been investigated. In this paper, using conditional RBM24 knockout mice, we demonstrated that ablation of RBM24 in postnatal heart led to rapidly progressive dilated cardiomyopathy (DCM), heart failure, and postnatal lethality. Global splicing profiling revealed that RBM24 regulated a network of genes related to cardiac function and diseases. Knockout of RBM24 resulted in misregulation of these splicing transitions which contributed to the subsequent development of cardiomyopathy. Notably, our analysis identified RBM24 as a splice factor that determined the splicing switch of a subset of genes in the sacomeric Z-disc complex, including Titin, the major disease gene of DCM and heart failure. Together, this study identifies regulation of RNA splicing by RBM24 as a potent player in remodeling of heart during postnatal development, and provides novel mechanistic insights to the pathogenesis of DCM.
ABSTRACT
At present, nonalcoholic fatty liver disease (NAFLD) is the most common liver disease, and is often regarded as the manifestation of metabolic syndrome in the liver. There are no specific drugs for this disease at the moment, and the only therapeutic method proved effective is guiding life style to reduce body weight. Insulin resistance, oxidative stress response, inflammatory response, and apoptosis are involved in the pathogenesis of NAFLD. Animal experiments and human studies have proved that various natural drugs have antioxidant, anti-inflammatory, or insulin-sensitizing effects and thus have significant efficacy in the treatment of NAFLD. This article summarizes the research advances in 10 natural drugs commonly used for the prevention and treatment of NAFLD in recent years and treatment of NAFLD in recent years and concludes that more studies are needed to confirm the efficacy of natural drugs in NAFLD patients.
ABSTRACT
Objective To investigate the clinical effect of using warm paste during cesarean section.Methods 300 cases underwent cesarean section in obstetric department of our hospital from January to June 2013 were collected,who were randomly divided into the experimental group and the control group with 150 cases in each group.The temperature,clinical curative effect and eliminating time of shiver reaction after cesarean section of two groups were observed.Results In the observation group,there were 92 cases (61.33％) whose temperature were higher than 36℃,23 cases (15.33％) whose temperature were less than 36℃,and compared with the control group,the difference in different temperature was statistically significant.The observation of shivering patients in two groups after treatment showed that the total effective rate of the experimental group was 94.29％,while that of the control group was 76.92％.The difference was statistically significant between two groups.The difference was statistically significant in the eliminating time of shiver reaction after treatment between two groups.Conclusions Using warm paste during cesarean section is safe and effective,and can significantly reduce complications.