ABSTRACT
Objective To investigate the expression of differential genes in testicular tissue of patients with obstructive and non-ob-structive azoospermia by bioinformatics,and provide new markers for the diagnosis of non-obstructive azoospermia(NOA).Methods The microarray data of azoospermia related genes(GSE45885 and GSE9210)were downloaded from the gene expression comprehensive database(GEO)and analyzed online by GEO2R,and the NOA related differentially expressed genes(DEGs)were obtained.The common DEGs were determined by using Wayne's intersection.GO annotation and KEGG enrichment analysis of DEGs were carried out by using R software.The DEGs-related protein interaction network(PPI)was constructed with STRING.Then,the most significant Hub gene of NOA was screened out by Cytoscape software and visualization was performed.The diagnostic value of Hub gene for NOA was estimated by the receiver operating characteristics(ROC)curve and verified in GSE145467 data set.Results A total of 83 DEGs were obtained,of which 78 were down-regulated and 5 were up-regulated.GO enrichment analysis showed that DEGs was involved in biological processes(BP),including the development and differentiation of sperm cells,development of germ cells,assembly of motor cilia,etc.Cell composition(CC)mainly included sperm flagella,motor cilia,acrosome vesicles,spermatogenic nuclei,etc.Molecular function(MF)mainly included structural components,protein binding,heat shock protein binding and so on,which endowed com-pressive strength for extracellular matrix.KEGG-related pathways were involved in longevity regulation pathways,cell cycle and apopto-sis,and meiosis of oocytes in multiple species.The five Hub genes closely related to NOA,including SPAG5,CCNB2,AURKC,NCAPH and PTTG1,were screened by PPI network.The ROC curve showed that all the five Hub genes were potential genetic markers of NOA.Conclusion SPAG5,CCNB2,AURKC,NCAPH and PTTG1 genes may play the key role in the development of NOA and may be used as the new biomarkers for NOA.
ABSTRACT
ObjectiveTo explore the clinical curative effect of different methods on anesthesia radical mastectomy. MethodsTo choose 120 patients with breast cancer,and divided into epidural group and general anaesthesia group according to different methods of anesthesia randomly, and monitored the changes of blood pressure, heart rate, blood oxygen saturation, norepinephrine, epinephrine and C-reactive protein of all patients during peroperative period, and analyzed the difference between the two groups. ResultsThe vital sign and level of stress hormone of patients of epidural group during peroperative period had no significant difference( all P ＜0.01 ). The vital sign and level of stress hormone of patients of general anaesthesia group intraoperation were significant different compared to preoperation and post operation( all P ＜ 0.01 ). ConclusionEpidural anesthesia had lighter stress reaction and stable vital sign than general anesthesia on radical mastectomy.