Construction of eukaryotic expression recombinant plasmid of human fibrinolysin / 吉林大学学报(医学版)

Objective To construct the eukaryotic expression vector of human fibrinolysin for further study on anti-thrombus and thrombolysis.Methods Human fibrinolysin gene was amplified from human fetal liver tissue with PCR,and was cloned into eukaryotic expression plasmid pCI-neo and then sequenced.After the correct identification by sequencing,the eukaryotic expression recombinant plasmid of human fibrinolysin was constructed and identified with PCR and enzyme digestion.Results The PCR amplification product of fibrinolysin gene was 1 740 bp.The result of sequencing was the same as that registered in GenBank.The results of PCR and enzyme digestion showed that the recombinant eukaryotic expression plasmid had correct codogenic gene fragment.Conclusion The eukaryotic expression recombinant plasmid of human fibrinolysin gene is successfully constructed and identified.

Determination of CD4~+CD25~+ regulatory T cells from peripheral blood in patients with multiple organ dysfunction syndrome and clinical significance / 吉林大学学报(医学版)

Objective To explore the change of CD4+ CD25+ regulatory T cells(Treg) from peripheral blood in patiens with multiple organ dysfunction syndrome(MODS) before and after treatment and its significance.Methods The frequencies of CD4+ CD25+ CD127-Treg were detected in 10 patients with MODS respectively before treatment and 1 week after treatment and 10 healthy donors by flow cytometry labeled with specific fluorescent antibodies,such as anti-CD4(PE-CY5),anti-CD25(FITC) and anti-CD127(PE).Results The frequency of CD4+ CD25+ CD127-Treg in patients after treatment(2.11%?0.33%) was significantly lower than before treatment(7.44%?1.59%)(P

Change of oxygen free radicals on the myocardial injury in acute multiple organ dysfunction syndrome(MODS) / 中华急诊医学杂志

Objective To investigate the effect of oxygen free radicals on the myocardial injury in acute MODS.Methods Thirty-six SD rats were randomly divided into two groups.The rats of experimental group were peritoneally injected with the suspension of zymosan-parogen.The rats of control group were injected with steriled saline at the same volume.The dynamtic change of LPO,SOD,myocardial enzyme in plasma and MDA,SOD in myocardial tissue at different time point were detected.The pathologic change of myocardial tissue by HE staining was observed.Results Compared with the control group,the LPO,MDA,CKMB,LDH,GOT in plasma and myocardial tissue increased at 6 hours (P