Comparation of Total Iridoids in Valerian from Different Habitats / 医药导报

Objective To determine and compare the contents of total iridoids in valerian from different origins.Methods The total iridoids in valerian was detected by reaction with alkaline hydroxylamine-perchloric acid iron reagent,and the absorbance was measured at 522 nm by spectrophotometry. Results The content of valepotriate and its absorption were linear within the range of 0. 05-1. 40 mg·mL-1 . The average recovery was 98. 50%,and RSD was 1. 02%. There was significant difference between the contents of iridoid in valerian from different habitats. Conclusion This method is accurate and reliable for evaluating the quality of valerianand. The contents of valeriana is related to its habitats.