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Journal of Zhejiang University. Medical sciences ; (6): 162-166, 2015.
Article in Chinese | WPRIM | ID: wpr-255217

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of irradiated human lung fibroblasts (HLFs) on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells (HUMSCs).</p><p><b>METHODS</b>HUMSCs were cultured alone (single group) or co-cultured with HLFs exposed to 5Gy X-rays (co-culture group). The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture. WISP-1 protein levels in conditioned medium were examined by ELISA.</p><p><b>RESULTS</b>The levels of p-GSK3β/GSK3β (0.15 ± 0.05), FRAT1 (0.48 ± 0.07) and β-catenin (0.50 ± 0.07) in co-cultured HUMSCs significantly decreased compared to those in single group (0.55 ± 0.05, 1.16 ± 0.13 and 2.39 ± 0.15, all P<0.05). The supernatant level of WISP-1 in co-culture group was significantly decreased [(602.23 ± 161.47) ng/mL], compared to the single group [(977.77 ± 110.56) ng/mL, P<0.05].</p><p><b>CONCLUSION</b>Irradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway in HUMSCs in vitro.</p>


Subject(s)
Humans , CCN Intercellular Signaling Proteins , Metabolism , Cells, Cultured , Coculture Techniques , Fibroblasts , Cell Biology , Radiation Effects , Gamma Rays , Glycogen Synthase Kinase 3 , Metabolism , Glycogen Synthase Kinase 3 beta , Intracellular Signaling Peptides and Proteins , Metabolism , Mesenchymal Stem Cells , Metabolism , Proto-Oncogene Proteins , Metabolism , Umbilical Cord , Cell Biology , Wnt Signaling Pathway , X-Rays , beta Catenin , Metabolism
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