Cloning,Expression of E2 Gene in Hepatitis C Virus Envelope Region and Significance of Anti HCV E2 Detection in Patients with HCV Infection / 中国输血杂志

Objective To clone and express the E2 gene in envelope region of hepatitis C virus and detect the anti E2 in sera of patients with HCV infection,using the purified E2 Protein.Methods The gene fragment of E2 region was obtained from the sera of patients with HCV infection by random primer reverse transcription and polymerase chain reaction(PCR),and then cloned into procaryotic expression system to express and Purify the protein and detect its antigenicity.Results A segment of E2 region gene with correct code reading frame was obtained,its full length was 984 bp and molecular weight of expressed Protein was 38kD.The detection of the protein performed by ELISA with sera from the patients with HCV infection,testified that it had appropriate antigenicity.Conclusion This experiment can provide the fundamental data for researching on the basic characteristics of HCV envelope protein,nuderstanding the significance of serological diagnosis and designing the anti HCV vassine.

Observation on the Hepatic Histology and Serology Changes in HCV-infected Blood Donors / 中国输血杂志

Seven asymptomatic blood donors,in whom the infection with HCV was verified,were selected. The time of their infection with HCV was one and a half years. And the research on the hepatic histology and serology indexes of the donors was performed. The liver tissue samples gotten by needle biopsy of liver ware examined under the common microscope and electron microscope,thereby the abnormal hepatic histology manifestation was found, in all the donors. The samples of seven cases have shown the pathological changes characteristic of early stage of viral hepatitis under microscope. The result of examination under electron microscope confirmed that the infected donors were located in early stage of infection,and showed the low grade hepatic histological lesion. The serological examination showed that all the seven cases had normal ALT level,and most of them were HCV RNA-positive and had genotype-II . It is necessary to conduct follow-up observation on the relationship between the changes of serological indexes and the hepatic lesion.

PROTEIN EXPRESSION AND ANTIGENICITY DETECTION OF HEPATITIS C VIRUS ENVELOPE PROTEIN E2 / 解放军医学杂志

Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems.The gene that encoding.HCV E2 protein was cloned in pQE30 and pEF1/HisC.After the expression of E2 protein in E.Coli M15 and COS 7 cell,the expressed proteins were used to detect their antigenicity with ELISA and WB.The results showed that protein E2 was expressed in both prokaryotic and eucaryotic cells.Special reaction could be detected using the expressed proteins and sera from HCV infected people.The studied E2 gene could express the desired proteins in both prokaryotic and eucaryotic expression systems,and glycosylation of the E2 protein happened in COS 7 cell.