ABSTRACT
Objective To detect the level of CD4+CD25+FoxP3+ (Treg) and Th1,Th2 cells in peripheral blood of patients with immune thrombocytopenia and discuss its clinical significance.Methods Flow cytometry was used to detect the percentage of Treg cells and Th1,Th2 cells in peripheral blood of 27 patients with ITP.25 healthy volunteers were detected as control group.Results The percentage of Treg cells in the peripheral blood of patients with ITP was (2.20 ± 0.93)％,which significantly decreased compared with the healthy control group (2.99 ± 0.45) ％ (t =3.820,P＜0.01) respectively,and the levels of Th1,Th2 cells were not statistically different between the two groups (tTh1 =0.655,tTh2 =0.467,all P＞ 0.05).Conclusion Treg cells in patients with ITP were decreased,and its important role in the development of ITP deserves further study.
ABSTRACT
BRG1 (Brahma-related gene 1, BRG1) is the ATPase subunit of SWI/SNF chromatin remodeling complexes, which plays an important role in cell cycle regulation, DNA repair and tumor development. Unlike the evidence as tumor suppressor genes in the past reports, latest researches show that BRG1 plays an important role in sustaining the growth of leukemia cells in acute myeloid leukemia, and these effects on normal hematopoietic stem cells are dispensable. Further studies of the role and mechanism of BRG1 in acute myeloid leukemia will contribute to the development of a new and promising targeted therapy strategy. This article reviews the role of BRG1 on leukemia cells and leukemia stem cells in AML and discusses the related mechanism, which providing some reference for the targeted treatment strategy of AML.
Subject(s)
Humans , Chromatin , Chromatin Assembly and Disassembly , DNA Helicases , Genetics , Leukemia, Myeloid, Acute , Genetics , Neoplastic Stem Cells , Cell Biology , Nuclear Proteins , Genetics , Transcription Factors , GeneticsABSTRACT
<p><b>OBJECTIVE</b>Recent studies have shown cardiac protection effects of erythropoietin (EPO). The present experiment was designed to investigate the effects of EPO on TGF-beta1, nitric oxide synthase (NOS), collagen contents induced by angiotensin II (Ang II) in rat cardiac fibroblasts (CFs) and explore the roles of PI3-K/Akt signaling pathway on related effects.</p><p><b>METHODS</b>Neonatal rat CFs was isolated by collagenase and trypsinase digestion methods. PBS, EPO, Ang II in the absence or presence of LY294002, an inhibitor of PI3-K, or L-NAME, an inhibitor of NOS, were added to CFs and cultured for 24 hours. The concentration of collagen I and collagen III in culture medium were quantitated by ELISA. The levels of nitric oxide (NO) and the activities of NOS as well as NOS isoforms were measured by chemical enzymic method. Western blot was applied to detecting the protein expressions of Akt, p-Akt, eNOS, iNOS, and TGF-beta1.</p><p><b>RESULTS</b>The concentrations of collagen I and collagen III in CFs culture medium were significantly increased while the level of NO was significantly decreased by Ang II and these changes were significantly suppressed by EPO in a dose dependent manner. The effects of EPO on eNOS and NO could be blocked by LY294002. L-NAME could block EPO's effect on NO but not on the eNOS expression. The suppression effects on expressions of TGF-beta1 and collagen by Ang II in CFs were blocked by both LY294002 and L-NAME.</p><p><b>CONCLUSION</b>EPO suppresses the upregulated expressions of TGF-beta1 and increased production of collagen induced by Ang II through activating the PI3-K/Akt signaling pathway in neonatal rat CFs.</p>