ABSTRACT
Objective To explore the relationship between the mean platelet volume (MPV) and varicocele, so as to contribute to the etiopathogenesis of varicocele. Methods The MPV values were examined in 50 varicocele subjects and 46 controls with benign non-varicocele diseases. Parameters including MPV and their relation with varicocelewere analyzed in the two groups. Results The average ageof patients at the timeof examination was (23. 50 ± 5. 08) yearsold for varicocele subjects and (24. 67±4. 33) for controls (P = 0. 599). MPV value for varicocele subjectswas significantly higher than that for controls (P= 0. 030). MPV value was found to be significantly correlated with varicocele grade (r= 0. 497, P = 0. 03). Conclusion The MPV value is increased in varicocele patients and the varicocele grade is positively associated with MPV value.
ABSTRACT
Background The orbital fibroblasts (OFs) in thyroid associated ophthalmopathy (TAO) play important roles in the proliferative and inflammatory response.Seeking the drug which inhibit OFs growth is of a vital significance for the prevention and treatment of TAO.Research documented that colchicine has an anti-fibrosis effect.But its influence on OFs of TAO patient is few known.Objective This study was to investigate the effect of colchicine on growth and apoptosis of OFs in vitro.Methods The retroobital connective tissue was obtained form 3 TAO patients and cultured using explant method.OFs were passaged and identified by immunochemistry,and 3-8 genetaions of cells were used in the study.Colchicine at the concentrations of 1 × 10-8,1 × 10-7,1 × 10-6,1 × 10-5,1 ×10-4 mol/L was added into the RPMI 1640 with 10％ fetal bovine serum(FBS) to incubated the cells for 24,48 and 72 hours respectively,and only RPMI 1640 was used to culture the cells as the control group.Cell counting kit-8 (CCK-8)was used to detect the absorbance value (A450) of OFs for the evaluatuion of OFs and the inhibitory rate of colchicine to OFs.The colchicine of 1 ×10-6,1 ×10-5,1 × 10-4 mol/L was added into the culture medium for 48 hours,and then the apoptotic rate of the cells and the cell percentage in various cellular cycle was assayed by flow cytometry(FCM).The expression of transforming growth factor-β (TGF-β)in the cells was detected by immunochemistry to assess the influence of colchicine on the serection of the cells.Results Cultured cells showed the spindle-like in shape and the cell number was significantly increased with the incubation time.After incubated with 1 × 10-4,1 × 10 5,1 × 10-6,1 ×10-7,1 × 10-8 mol/L colchicines,the A450 values were gradually reduced with the increase of the concentrations of colchicine(F ion =62.004,P＜0.05),and significant differences were found between different contrations of colchicine groups(all P＜0.05).Aslo,gradually declined A450 values of the cells were seen with the lapse of culture time among the groups(Ftime =459.582,P＜0.05).The inbitory rate of colchicine to the cells was elevated with the increase of concentrations.The apoptotic rates of the cells were (1.73 ± 0.15) ％,(21.04 ± 4.56) ％,(31.84 ±6.21)％and(35.32±5.56)％ in the control group and 1 × 10-6,1 × 10-5,1 × 10 4 mol/L colchicine groups respectively,with statistically significant difference among the 4 groups (F =83.905,P＜0.05).With the increase of concentrations of colchicines,the cell percentage in G2 +M phase lessened gradually,showing significant difference among the control group and the 1 × 10-6,1 × 10-5,1 × 10-4 mol/L colchicine groups (F =20.443,P＜0.05).The expression of the TGF-β in the cells was (97.60± 2.09) ％ in the control group,and that in the 1 × 10-4 mol/L colchicine group was (44.43 ± 3.96) ％,presenting a significant difference between them (t =65.330,P ＜ 0.05).Conclusions Colchicine can induce apoptosis of OFs and inhibit the prolilferation of OFs in a time-and dose-dependent manner probably by decreasing the TGF-β secretion
ABSTRACT
Background Inflammation and adipogenesis are two parallel processes with increasing activity in severe thyroid-associated ophthalmopathy(TAO),and mevastatin was proved to have the inhibiting effect on the differentiation of adipose.Objective The aim of this work was to investigate the effects of mevastatin on the expression of cyclooxygenase-2(COX-2)and peroxisome proliferator activated receptor-γ(PPAR-γ)and differentiation of TAO-derived orbital preadipocytes,and explore its modulation effects on lipopolysaccharide(LPS)induced inflammation and the differentiation of TAO-derived orbital preadipocytes in vitro.Methods The retroorbital adipose tissue was obtained from 4 TAO patients during the surgery.The orbital fibroblasts were cultured from orbital adipose tissues using explant culture method.To study the suppressing effect of mevastatin on inflammatory response,cultured cells were divided into 5 groups.The 1000 μg/L LPS orbital fibroblasts were stimulated for 8 hours in group A,and 1000 μg/L LPS combined with 5 μmol/L,10 μmoL/L or 20 μmoL/L mevastatin were used respectively for the substitute in the group B,group C and group D.The orbital fibroblasts in group E were cultured routinely without any intervention as control.To observe the inhibiting effect of mevastatin on the differentiation of adipose,the group A were then subdivided into group A1-A6.After 1000 μg/L LPS was used to treat the cells for 8 hours,the ceils were induced to differentiate into adipocytes.All orbital preadipocytes from A1 to A6 were stimulated to differentiate into mature adipocytes with cocktail differentiation medium for a 10-day duration.During the procedure,group A2,A3 and A4 were interfered with 5,10 or 20 μmol/L mevastatin,and in the group A5 and A6,10 μmol/L mevastatin were added at the fourth day or eighth day.Intracellular fat accumulation in differentiated adipocytes was determined by oil red O staining.The absorption(A492 nm)was measured in the ceils by enzyme-linked immunosorbent assay(ELISA).Expression of COX-2 and PPAR-γ mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR),and the expression of COX-2 and PPAR-γ protein was detected by Westernblot.The level of PGE2 in the supernatant was detected by ELISA.Results The expression of COX-2 protein and mRNA as well as the PGE2 levels in B,C,D group decreased markedly in comparison with those in A group(P＜0.05).With the increase of mevastatin concentration,the expression of COX-2 protein and mRNA as well as the PGE2 levels in B,C,D groups decreased successively(F =228.380,101.745,1586.881,P＜0.05).The expression of COX-2 protein and mRNA and PGE2 levels in E group were lower significantly than those in A,B and C groups(P＜0.05),but no significant differences were found between E group and D group(P＞0.05).The A492 value and the expressions of PPAR-γ protein and mRNA in differentiated cells showed the successively decrease in A1-A4 group with the elevation of mevastatin concentration(P＜0.05),and the evidently decreased A492 value and the expressions of PPAR-γ protein and mRNA also were seen in A1 and A5 groups compared with A3 group(P ＜ 0.05).Conclusions Mevastatin inhibits LPS-induced COX-2 expression,PPAR-γ expression,PGE2 secretion and differentiation of TAO-derived orbital fibroblasts in vitro in dose-dependent manner.Mevastatin plays these effect more prominently in early stage of adipocytes differentiation.
ABSTRACT
OBJECTIVE@#To study the cell surface antigen expression of orbital preadipocytes between patients with thyroid-associated ophthalmopathy (TAO) and healthy adults.@*METHODS@#Tissue culture Methods were used in the cell culture of orbital preadipocytes in patients with TAO and healthy adults. Flow cytometry was used to analyze the expression of CD29, CD44, CD49d, and HLA-DR in these 2 cells.@*RESULTS@#Positive expressions were examined in orbital preadipocytes of patients with TAO and healthy adults. The orbital preadipocytes of patients with TAO had a higher expression of HLA-DR than that of healthy adults.@*CONCLUSION@#The orbital preadipocytes of patients with TAO and healthy adults cultured by tissue cultured Methods both belong to mesenchymal stem cells, and the former may participate in the orbital inflammation.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Adipocytes , Allergy and Immunology , Antigens, Surface , Allergy and Immunology , Metabolism , Cells, Cultured , Graves Ophthalmopathy , Allergy and Immunology , Metabolism , HLA-DR Antigens , Allergy and Immunology , Hyaluronan Receptors , Allergy and Immunology , Integrin beta1 , Allergy and Immunology , Orbit , Allergy and Immunology , MetabolismABSTRACT
OBJECTIVE@#To evaluate the Methods , complication and operative skill in transconjunctival surgical cryoextraction of intraconal orbital cavernous hemangioma.@*METHODS@#Among the 41 patients diagnosed as orbital cavernous hemangioma preoperatively, 36 suitable for the condition were treated with the operation. The operation time was recorded, and the complications were observed during and after the operation.@*RESULTS@#The tumours of the 36 patients were all excised successfully, of which 35 were diagnosed as orbital cavernous hemangioma, and the other 1 as neurilemoma post-operatively. The operation time of each was 20 to approximately 25 min and there was no perpetual complications.@*CONCLUSION@#Transconjunctival surgical cryoextraction of intraconal orbital cavernous hemangioma is simple, swift, safe and effective with tiny injury.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Conjunctiva , General Surgery , Cryosurgery , Methods , Hemangioma, Cavernous , General Surgery , Ophthalmologic Surgical Procedures , Methods , Orbital Neoplasms , General SurgeryABSTRACT
OBJECTIVE@#To evaluate the therapeutic efficacy of suture-fixation of foldable intraocular lens for non-posterior-capsular aphakia.@*METHODS@#Akreos intraocular lens (from Bausch-Lamb Company) were selected for operation in aphakia with non-posterior-capsule or less capsule supporting intraocular lens. Complications and visual acuity were observed.@*RESULTS@#There was no significant complication in the operation. Choroidal detachment occurred in 2 eyes postoperatively. Visual acuity of 0.3 accounted for 56.67%, 76.67%, 86.67%, and 90% respectively on the first day, at 1 week, 1 month and 3 months after the operation.@*CONCLUSION@#Suture-fixation of Akreos intraocular lens is a good choice for non-posterior-capsular aphakia.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Aphakia , General Surgery , Aphakia, Postcataract , General Surgery , Lens Implantation, Intraocular , Methods , Lenses, Intraocular , Ophthalmologic Surgical Procedures , Methods , Suture TechniquesABSTRACT
OBJECTIVE@#To investigate the clinical manifestation and pathological features of primary ocular adnexal non-Hodgkin lymphoma.@*METHODS@#Data of 18 patients with biopsy-proven ocular adnexal non-Hodgkin lymphoma at Xiangya hospital were reviewed.The disease site, clinical manifestation,imaging and pathological features of the tumor were summarized.@*RESULTS@#All patients had typical presentation of an adnexal mass.Twelve(66.7%) patients had orbital involvement, 3(16.7%) had conjunctival, and 3(16.7%) had lymphoma involving the eyelids.Eight patients were misdiagnosed as "inflammatory pseudotumour" before the operation according to their clinical and imaging examination,another 8 patients were diagnosed as "ocular adnexal tumour with unknown nature" before the operation. According to the pathologic diagnosis, 16 patients (88.9%)had marginal zone lymphomas of mucosa-associated lymphoid tissue(MZL-MALT) and 2 (11.1%) had NK/T-cell lymphoma.@*CONCLUSION@#The typical presentation of ocular adnexal lymphoma is a painless mass.Orbital connective tissue is the most involved anatomical site. The diagnosis of ocular adnexal non-Hodgkin lymphoma is difficult which could easily be misdiagnosed as "inflammatory pseudotumour". MZL-MALT is the most common pathologic type.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Diagnosis, Differential , Eye Neoplasms , Diagnosis , Pathology , Lymphoma, Non-Hodgkin , Classification , Diagnosis , Pathology , Orbital Neoplasms , Diagnosis , PathologyABSTRACT
OBJECTIVE@#To investigate histopathologic and ultrastructural changes of extraocular muscles (EOM) in thyroid associated ophthalmopathy (TAO).@*METHODS@#Twelve EOM specimens from 11 patients with TAO were observed. Each of the specimen was stained with HE and observed by light microscope,and then was sectioned with ultrathin method and observed by transmission electronic microscope.@*RESULTS@#Under the light microscope, sarcoplasm coagulation,granular degeneration, vacuolization and necrosis were found in the extraocular muscular cells.Under the electronic microscope, there were disturbance and disappearance of the Z line in part of muscular fibers and various degrees of sarcoplasmic reticulum dilatation, myofilament lysis and destruction with formation of vacuoles.In severe cases,the muscular cells could be completely destroyed and phagocytosed by macrophages,fibrosis occurred and myofibroblasts were found in some cases.@*CONCLUSION@#The extraocular muscles in TAO are destroyed at various degrees,and the muscular cells may be the target cells in TAO.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Graves Ophthalmopathy , Pathology , Oculomotor Muscles , PathologyABSTRACT
OBJECTIVE@#To evaluate the clinical effects of the mixture of 5-fluorouracil (5-FU) and triamcinolone acetonide on capillary hemangioma of eyelid.@*METHODS@#One hundred and one patients with capillary hemangioma of eyelid were divided into Group A and Group B: Group A was injected with triamcinolone acetonide, and Group B was injected the mixture of 5-FU and triamcinolone acetonide.@*RESULTS@#The cure rate was 68.3%, the total effective rate was 76.0%, and the average course of treatment was (8.1+/-3.4) months for Group A; the cure rate was 90.0%, the total effective rate was 96.6%, and the average course of treatment was (5.1+/-2.3) months for Group B. The therapeutic effect in Group B was better than that in Group A (P<0.05). The treatment period in Group B was shorter than that in Group A (P<0.05).@*CONCLUSION@#5-FU combining with triamcinolone acetonide has not only a better therapeutic effect, but also a shorter period of treatment.