ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Tiaomaiyin injection on the experimental arrhythmia for analyzing its underlying mechanism in the treatment of cardiovascular disease.</p><p><b>METHOD</b>Experimental animals anesthetized with 20% urethane (6 mL x kg(-1)) were evenly randomized into control group, positive control group, low-dose and high-dose Tiaomaiyin group. The rate of ventricular fibrillation (VF) chloroform-induced in mice, and the epoch of ventricular extrasystole (VE), ventricular tachycardia (VT),VF and cardiac arrest (CA), actonitine-induced in rats (1.0 microg x mL(-1) x min(-1)), and vabain-induced in guinea pigs (10 microg x mL(-1) x min(-1)), were detected respectively. The result loas converted into cumulative dosage of actonitine or vabain. In ischemia-reperfusion model in rats, the duration of arrhythmia and activity of superoxide dismutase (SOD) and malondialdehyde (MDA) were detected.</p><p><b>RESULT</b>After venous injection of Tiaomaiyin, VF in mice was lower significantly (P < 0.01), VE, VT, VF in rats and VF in guinea pigs were lowered considerably (P <0.05). The duration of arrhythmia in ischemia-reperfusion model was reduced considerably (P < 0.05), and the activity of myocardial SOD was raised significantly (P <0.01).</p><p><b>CONCLUSION</b>Tiaomaiyin shows the reduction of experimental arrhythmia and protect effect to ischemia-reperfusion injury of the heart, which indicates that the effect mechanism may have the relationship with inhabition of lipid peroxidation and damnification of the free radical.</p>
Subject(s)
Animals , Mice , Rats , Anti-Arrhythmia Agents , Pharmacology , Arrhythmias, Cardiac , Metabolism , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Electrocardiography , Guinea Pigs , Injections , Malondialdehyde , Metabolism , Myocardial Ischemia , Myocardial Reperfusion Injury , Metabolism , Myocardium , Metabolism , Plants, Medicinal , Chemistry , Random Allocation , Rats, Sprague-Dawley , Superoxide Dismutase , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the morphologic feature, immunohistochemistry phenotype of ESS and its metastases, with emphasis on the histogenesis, tumor differentiation and diagnostic criteria.</p><p><b>METHODS</b>The pathologic features of 15 cases and 4 metastases were studied. The immunohistochemical study was performed on selected sections by a panel of antibodies including CD10, smooth muscle actin, estrogen and progesterone receptors, keratin (AE1/3) and alpha-inhibin.</p><p><b>RESULTS</b>Patients were 22 to 75 years of age (mean 45). The endometrial stromal component predomominated in 7 cases. Three cases showed a picture of smooth muscle differentiation. Endometrial stromal sarcoma with fibromyxoid features were present in 2 cases. There were 3 sarcomas of poorly differentiated. The morphology features of the metastatic foci in 3 of the 4 metastasis cases were not similar to that of the primary counterpart in uteri. Among 14 ESS and 4 metastases, 15 of 18, 5 of 18, 7 of 18, and 10 of 18 were positive for CD10, SMA, ER and PR, respectively. AE1/3 and alpha-inhibin were only positive in the adenomatous area of ESS. Strong expression of SMA was obtained in all 10 cellular leiomyomas, and CD10 was only weakly expressed in 1 case (P < 0.05).</p><p><b>CONCLUSIONS</b>ESS are morphologically heterogeneous with multipotential differentiation. The histologic features of the metastases may not be similar to those of the primary. CD10 and SMA are diagnostically useful markers for ESS.</p>
Subject(s)
Adult , Aged , Female , Humans , Middle Aged , Actins , Metabolism , Cell Differentiation , Endometrial Neoplasms , Metabolism , Pathology , Lung Neoplasms , Diagnostic Imaging , Metabolism , Muscle, Smooth , Pathology , Neoplasm Recurrence, Local , Neprilysin , Metabolism , Radiography , Sarcoma, Endometrial Stromal , Metabolism , Uterus , Metabolism , PathologyABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Jisheng injection (JSI) in protecting heart.</p><p><b>METHODS</b>Isolated heart of rat was preserved in modified Euro-Collins solution (mEC) containing JSI for 20 hrs, and that preserved in simple mEC was taken as control. Then Langendorff isolated rat heart perfusion was conducted. Forty minutes after perfusion, the cardiac function, coronary flow, myocardial water content were determined, and lactate dehydrogenase (LDH), creatine kinase (CK) activity in perfusate, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content in myocardial tissue and pathologic change in myocardium were also observed.</p><p><b>RESULTS</b>The cardiac function and coronary flow of isolated heart preserved in JSI containing mEC was significantly better than those in the control (P < 0.01), with the LDH, CK activity and MDA content significantly lower (P < 0.01 and P < 0.05), SOD activity significantly higher (P < 0.05) and pathologic injury milder than those in control, but comparison of cardiac water content between the two groups showed insignificant difference.</p><p><b>CONCLUSION</b>JSI has good cardiac protective and anti-oxidizing effects.</p>