ABSTRACT
Objective To compare the clinical values of impedance method,optical method and microscopy when used to detect platelet abnormal results.Methods Platelet re-examinations by optical method and microscopy were carried out in case of low confidence degree in platelet test with impedance method by XE-2100 automatic hematology analyzer,and then the results by the three methods were compared.Results Most of the low-confidence-degree results by impedance method could be corrected by re-examination by optical method,and the remained had to turn to microscopy due to unsatisfied requirements of the instrument.Conclusion Optical method has to be involved to correct the platelet abnormal results by XE2100 automatic hematology analyzer,and microscopy should be applied in case optical method doesn't work.The three methods gains advantages and disadvantages of themselves,and can be supplementaries for one another.
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Objective To verify the effect of different centrifugal conditions on coagulation and ascertain the optimum centrifugal time for coagulation testing in the laboratory .Methods Navy General hospital check‐up and hospitalized patients were divided into three groups which were conventional group ,standard group and speed up the group respectively according the different centrifuga‐tion conditions .The platelet poor plasma (PPP) was harvested in the three different groups .The PT ,INR ,FIB and APT T were tested and the results were compared and analyzed .Samples with different platelets were centrifuged in normal condition or standard condition ,after that ,the residual platelet in plasma was detected .Results There was no significant difference of the PT ,INR ,FIB and APT T results among the routine group ,speed group and the standard group .The results of PPP was qualified 100% in samples which platelets were less than 500 × 109 /L with routine conditions and standards of centrifugal conditions .However ,the qualified result of PPP was dropped to 64% in those samples which platelets were higher than 500 × 109 /L with routine and standards of centrifugal conditions .Conclusion The conventional centrifugation conditions can meet clinical routine coagulation testing require‐ments ,the special types of coagulation assays or the samples which platelets count are more than 500 × 109 /L should centrifuge in accordance with the requirements of the CLSI centrifugal processing .
ABSTRACT
OBJECTIVE To study the pathogenicity of the Vibrio fluvialis isolated from the coastal seawater.METHODS Virulence experiment group:22 Kunming mice were divided into four subgroups in random:V.fluvialis was injected into abdominal cavity in the test subgroup.And Staphylococcus aureus and Escherichia coli were injected into the positive control subgroups,separately and aseptic physiological saline was injected into the negative control group.Wound infection group:22 SPF mice were divided into four supgroups in random after their legs were injured:the experimental supgroup(soaked in artificial seawater with V.fluvialis);the positive control groups(with S.aureus and E.coli,separately);the negative control group(soaked in aseptic artificial seawater).The general condition,blood routine,blood culture,organ culture and wound secretion culture of the mice were observed.The pathological analysis of the mice was taken after sacrifice on the 3rd day.RESULTS In virulence experiment group,among all the 7 mice′s blood culture of V.fluvialis supgroup,5 mice were found V.fluvialis positive after 12 h injection,and 2 mice kept on positive until 24 h.In wound infection group,pathological examination showed there were a large number of neutrophils distributed over the striated muscle of the injured sites and cellulitis formed.CONCLUSIONS The V.fluvialis isolated from the sea water has pathogenicity,and can cause wound) infection and septicemia when the concentration reached 106 CFU/ml.