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Objective:To investigate the relationship between gene polymorphisms of disease-relevant multiple cytokines including TNF-α,IL-6,IL-10,TGF-β1,IFN-γand acute graft versus host disease(aGVHD) in allogeneic hematopoietic stem cell trans-plantation ( allo-HSCT ) . Methods:32 cases of recipients received allo-HSCT and 36 cases of normal groups in January 2014 to December 2015 were selected as objects of study. We detected genotypes on specific SNP of target genes by polymerase chain reation ( PCR) combined with gene sequencing and observed the occurrence of aGVHD in postoperative recipients. The influence of cytokine gene polymorphisms on prognosis of allo-HSCT patients was analyzed,and the potential relationship between specific SNP mutation of the disease-relevant cytokine genes and severity of aGVHD was discussed. Results:Distribution of cytokines gene polymorphism including TNF-α-308(G/A),IL-6-174(G/C),IL-10-1082(A/G),TGF-β1+915(G/C),IFN-γ(T/A) had no significant differences with incidence of severe aGVHD(P>0. 05). However,the occurrence of severe aGVHD in allo-HSCT recipients with C/T genotype was significantly higher than C/C and T/T in SNP of TGF-β1+869(P<0. 01). Conclusion:Gene polymorphism of TGF-β1+869(C/T) in allo-HSCT patients was closely related to the occurrence of severe aGVHD. The research show allo-HSCT patients with C/T genotype occurred severe aGVHD more frequently, which is an important potential risk factor to induce the incidence of severe aGVHD. Therefore,detecting gene polymorphism of TGF-β1+869 ( C/T ) in allo-HSCT recipients and developing the appropriate therapeutic regimen may be helpful to reduce the incidence of aGVHD.
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OBJECTIVE:To investigate the significance of tacrolimus blood concentration monitoring to the therapy of mem-branous nephropathy. METHODS:41 patients with membranous nephropathy received tacrolimus,and the blood concentration of ta-crolimus reached to steady state. The trough concentration of tacrolimus was determined by EMIT. The patients were followed up, and clinical therapeutic efficacies were recorded. The relationship of blood concentration of tacrolimus with clinical efficacy was evaluated by SPSS 16.0 software. RESULTS:The blood concentration of tacrolimus was(7.47±2.74)ng/ml in complete remission (CR)group,(5.72±1.19)ng/ml in partial response(PR)group,and(3.30±1.08)ng/ml in no response(NR)group,with total remission rate of 75.61%. The blood concentration of CR group was the highest,followed by PR group and NR group,there was statistical significance among 3 groups(P<0.05). CONCLUSIONS:The clinical efficacy of tacrolimus in the treatment of nephrot-ic syndrome is correlate to the blood concentration intimately. Trough concentration monitoring of tacrolimus has important signifi-cance to the treatment of membranous nephropathy.
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Abstract: Lipoproteins are biological lipids carriers. The natural and reconstituted lipoprotein based drug delivery systems have been extensively developed in recent years. This article reviews the development of natural and reconstituted low-density lipoprotein and high-density lipoprotein based vehicles in the antitumor area.
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Objective To explore the degree of the improvement for hypertension patients with dyslipidemia by amlodipine combination atorvastatin treatment, and analyze the influence on vascular function.Methods 186 hypertension patients with dyslipidemia were selected.According to random number table,they were divided into the control group (91 cases) and the treatment group (95 cases),the control group was given amlodipine treatment,the treatment group was given Atorvastatin Calcium Tablets on the basis of the control group.The main indexes were the difference of systolic and diastolic blood pressure before and after the treatment,and high resolution detection of carot-id artery intima media thickness ( IMT) and carotid plaque area by color Doppler ultrasoundand.Serum total choles-terol ( TC) ,triglyceride ( TG) ,low density lipoprotein cholesterol ( LDL-C) and blood viscosity,hematocrit,erythro-cyte aggregation index were compared the change degree before and after treatment.Results The differences of pre and post-treatment on the systolic and diastolic blood pressure of the treatment group were (25.76 ±4.81)mmHg and (12.40 ±3.66)mmHg,higher than those of the control group,the difference was statistically significant (t=9.48, 8.76,all P<0.05).The differences of pre and post-treatment on IMT and plaque area of the treatment group were (0.10 ±0.11) mm and (0.33 ±0.14) mm2,higher than those of the control group,there was statistical significant differences (t=5.40,5.93,all P<0.05).The differences of pre and post-treatment on TC,TG and LDL-C of the treatment group were(1.06 ±0.38)mmol/L,(0.76 ±0.31)mmol/L and (0.58 ±0.20)mmol/L,higher than those of the control group,the difference was statistically significant (t=6.85,6.13,7.02,all P<0.05).The differences of pre and post-treatment on plasma viscosity,whole blood viscosity,hematocrit and erythrocyte of the treatment group were (0.36 ±0.08)Pa· s,(0.51 ±0.14)Pa· s,(0.41 ±0.12) and (0.31 ±0.12),higher than those of the con-trol group,the difference was statistically significant (t=5.81,4.97,5.11,6.86,all P<0.05).Conclusion The combination of amlodipine and atorvastatin on hypertension patients with dyslipidemia could reduce blood pressure and blood fat significantly,improve blood rheology,which has a good protective effect on vascular function.
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Objective To investigate expressions of the vascular endothelial growth factor (VEGF) family and their receptors in cardiac repair/remodeling after myocardial infarction (MI).Methods The infarcted rat heart model were constructed,real time PCR (RT-PCR) and Western blots (WB) were used.Results Compared to the normal myocardium,VEGF-A was significantly decreased in MI group during the 42 days observation period but decreased at day 1,which was 0.89 ±0.04 of control group in D1,0.25 ±0.03 of control in D14; VEGF-B was significantly suppressed in the infarcted heart,which level was 0.09 ± 0.04 of control; However,VEGF-C and VEGF-D were markedly increased in the infarcted heart in MI group,which was 5.31 ± 0.21 and 9.24 ± 0.47 times of control.Meanwhile,VEGFR-1 and 2 were 0.11 ± 0.02 and 0.14 ± 0.04 of control in the infarcted heart,but VEGFR-3 was significantly increased in blood vessels,6.81 ± 0.42 times of control group.Conclusions VEGF isoforms and VEGFR subtypes were differentially expressed in the infarcted heart.It suggests that these isoforms may regulate multiple responses during cardiac repair/remodeling.