ABSTRACT
Researches on detection of human papillomavirus (HPV) high-risk samples were carried out by poly-merase chain reaction (PCR) coupled with microchip electrophoresis (MCE). Herein, we introduced a simple, rapid, automated method for detecting high-risk samples HPV16 and HPV18. In this research, general primers were initially selected to obtain sufficient detectable yield by PCR to verify feasibility of MCM method for HPV detection, then type-specific primers were further used to evaluate the specificity of MCE method. The results indicated MCE method was capable of specifically detecting high-risk HPV16 and HPV18, and also enabled simultaneous detection of multiplex samples. This MCE method described here has been successfully applied to HPV detection and displayed excellent reliability demonstrating by sequencing results. The inherent capability of MCE facilitated HPV detection conducted in a small chip with automated, high throughput, massive parallelized analysis. We envision that MCE method will definitely pave a way for clinical diagnosis, and even on-site screening of cervical cancer.
ABSTRACT
OBJECTIVE:To provide reference for rational use of Fentanyl transdermal patch in the clinic. METHODS:By ex-tracting data from hospital information system and digital medical record management system,medical records of 150 cancer pain inpatients receiving Fentanyl transdermal patch were collected from our hospital during Jun.-Dec. 2014,and then investigated and analyzed retrospectively. RESULTS:Numeric rating scale (NRS) score of 150 cancer pain patients receiving Fentanyl transdermal patch were as follows:pain scores of 17 patients (11.3%) were 1-3 points,those of 63 patients (42.0%) were 4-6 points and those of 5 patients(3.3%)were 7-10 points;the rest of the patients were not rated. Among those 150 cases,88 cases were well tol-erated with opioid and 62 patients were considered to be opioid intolerant. Those intolerant patients were who could not be treated orally or could not bore side effects of opioid hepatic or renal dysfunction. 25 cases used Fentanyl transdermal patch without defi-nite evidence. As to drug combination,22 cases additionally received Morphine hydrochloride injection;12 cases additionally re-ceived Bucinnazine hydrochloride injection;8 cases additionally received Morphine tablet;10 cases additionally received Oxycodo-ne hydrochloride sustained-release tablets and Morphine hydrochloride sustained-release tablets;8 cases additionally received Oxyco-done and acetaminophen tablets;3 cases additionally received Carbamazepine tablets. CONCLUSIONS:Fentanyl transdermal patch are widely used for cancer pain treatment in our hospital. There still is irrational use of it,such as absence of pain score before use,no medication indication,irrational drug combination. Physicians should obey medication indication strictly,and clinical phar-macists should strengthen communication with physicians and pay attention to medication education for patients so as to guarantee rational drug use.
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A new biosensor was prepared based on conductive polymer poly ( 3 , 4-ethylenedioxythiophene ) ( PEDOT) and 1-pyrenebutanoic acid ( PBA) through π-π stacking, as well as hematin associated with PBA through coordinate bonds of Zr4+ and carboxyl group. Its stability and sensitivity were examined by cyclic voltammetry ( CV) , electrochemical impedance spectroscopy ( EIS) and current-time ( i-t) method. A pair of well defined and quasi-reversible redox peaks was observed when GCE/PEDOT/PBA/hematin was tested by CV in PBS without oxygen. The electron transfer rate constant was estimated to be 4. 8 s-1 . Results indicated that the PEDOT film enhanced the electron transfer process of hematin. When the i-t method was used to detect the response of biosensor to catechol with different concentrations, it displayed a linear response for the reduction of catechol in the range of 0. 5-200 μmol/L. The linear equation was i=0. 018C+0. 006 ( R=0. 9998), and the detection sensitivity was 0. 258 μA (μmol/L· cm2) with a detection limit of 0. 33 nmol/L (S/N=3). The results illustrate that the GCE/PEDOT/PBA/hematin biosensor is very sensitive and stable.
ABSTRACT
Objective To detect plasma concentrations of Calcitonin gene-related peptide (CGRP),endothelin (ET)-1 at different periods after subarachnoid hemorrhage(SAH),and probe the correlation of CGRP,ET-1 and the pathogenesis of cerebral vasospasm after SAH.Mehods Plasma concentrations of CGRP,ET-1 were measured in patients who were diagnosed with SAH (38 cases) at different periods (1-3 d,4-7 d,14-21 d after SAH) by radio-immunity technique.According to the occurrence of cerebral vasospasm,these patients were separated into two groups:vasospasm group(31 cases) and non-vasospasm group(7 cases).Twenty healthy persons after physical examinations were allocated to be control group.Results Compared with control group and nin-vasospasm group,the plasma concentration of CGRP in vasospasm group was obviously lower,especially in the 4-7 d time slot,and ET-1 was obviously higher,especially in the 4-7 d time slot.There were statistical differences among the three groups(P<0.05 or <0.01).Cerebral vasospasm occurred in 2 cases during 1-3 d,in 28 cases during 4-7 d and in l case during 14-2l d after SAH.Conclusions CGRP,ET-1 participate in the pathological process of SAH.The abnormality of vascular endnfhelial construction/diastolic function is correlated with delayed cerebral vasospasm.