ABSTRACT
This study aims to determine the phytochemical composition and antioxidant activity of air-dried Cordia dichotoma seeds. Total polyphenolic content was analyzed via the Folin-Ciocalteu method. Total triterpenoid content and amino acids was analyzed colorimetrically
The rosmarinic acid content was examined using high-performance liquid chromatography tandem mass spectrometry
The ethanolic extracts contained polyphenolic compounds [1.0%], triterpenoids [0.075%], amino acids [1.39%], and rosmarinic acid [0.0028%]. The results from this study indicate that C. dichotoma seeds are a rich source of polyphenolic compounds and amino acids, which can be used for quality assessment. The ethanolic extract of C dichotoma seeds has good antioxidant capacity
ABSTRACT
Objective To establish an HPLC method for the content determination of gallic acid and kaempferol-3-O-rutinoside inNymphaea candida Presl..MethodsThe separation was carried out on a Wondasil C18 column (250 mm×4.6 mm, 5μm). The mobile phase was methanol-0.1% acetic acid solution, with gradient elution;the flow rate was 1.0 mL/min;the detection wavelength was set at 270 nm;the column temperature was 30℃.Results The linear ranges of gallic acid and kaempferol-3-O-rutinoside were 0.065-0.585μg (r=0.999 1), 0.133-1.197μg (r=0.999 5), respectively. The average recoveries of gallic acid and kaempferol-3-O-rutinoside were 102.71%, 102.08%, with RSD of 1.97%, 0.46%, respectively.Conclusion This method was rapid, accurate, and reliable. It can be used for the determination of allic acid and kaempferol-3-O-rutinoside in Nymphaea candidaPresl..
ABSTRACT
Objective To analyze and compare the essential oil ofZiziphora clinopodioides Lam. from different regions in Xinjiang by infrared spectroscopy;To provide a reference for its identification and quality control.Methods Characteristic absorption peaks ofZiziphora clinopodioides Lam. essential oil from 18 regions in Xinjiang Uygur autonomous region were identified, compared and analyzed according to peaks’ shapes, positions and intensities by infrared spectroscopy, second derivative infrared spectroscopy and clustering analysis.Results Differences exist in the characteristic absorption peaks according to peaks’ shapes, positions and intensities. And then the samples from 18 regions can be divided into four categories.Conclusion This method was direct, simple, fast and convenient. Combining with clustering analysis, It could provide a reliable evidence for identification and quality control ofZiziphora clinopodioides Lam..