ABSTRACT
Objective To explore the influences of sufentanil and different dose midazolam coprocessing on myocardial injury in rats during myocardial ischemia-reperfusion (IR ). Methods Forty two healthy male SD rats were selected and randomly divided into 7 groups including,group A:rats were treated with sham operation;groups B0-B5:rat IR model was con-structed;group B0:rats were untreated with sufentanil or midazolam;group B1:rats were per-trea-ted with 3 μg/kg sufentanil;group B2:rats were post-treated with 0.1 mg/kg midazolam;group B3:rats were post-treated with 0.3 mg/kg midazolam;group B4:rats were per-treated with 1.5 μg/kg sufentanil and post-treated with 0.05 mg/kg midazolam;group B5:rats were per-treated with 1.5μg/kg sufentanil and post-treated with 0.15 mg/kg midazolam.Sufentanil was injected through cervi-cal vein before ischemia.Midazolam was injected through cervical vein after ischemia.After reperfu-sion,arterial blood was collected for detecting the activity of CK-MB,LDH or SOD,and the concen-tration of MDA,TNF-α,or IL-6.Rat hearts were picked off to measure the severity of myocardial in-farction (IS/AAR).The expressions of Bax,cleaved-caspase-3 and Bcl-2 in myocardial tissues were detected by Western blots.Results CK-MB activity,LDH activity,MDA concentration,TNF-αlev-el,IL-6 level,Bax and cleaved-caspase 3 expression levels in groups B0-B5 were obviously higher than those in group A (P <0.05),in groups B1-B5 were obviously lower than those in group B0 (P<0.05),in groups B3 and B4 were obviously lower than those in group B2 (P <0.05),in group B5 were obviously lower than those in groups B1-B4 (P < 0.05 );values of IS and IS/AAR (%)in groups B1-B5 were obviously lower than those in group B0 (P <0.05),in groups B3 and B4 were ob-viously lower than those in group B2 (P < 0.05 ),in group B5 were obviously lower than those in groups B1-B4 (P <0.05 );SOD activity and Bcl-2 expression level in groups B0-B5 were obviously lower than those in group A (P <0.05),in groups B1-B5 were obviously higher than those in group B0 (P <0.05),in groups B3 and B4 were obviously higher than those in group B2 (P <0.05 ),in group B5 were obviously higher than those in groups B1-B4 (P <0.05).CK-MB declined when com-pared with group B0;LDH declined;SOD rose;MDA declined;TNF-αdeclined;IL-6 declined;IS/AAR declined;Bcl-2 expression rose to 2.25 fold;Bax expression dropped to 54.89%;cleaved-caspase-3 expression dropped to 49.67%.Conclusion Sufentanil pretreatment combined with mid-azolam aftertreatment can significantly alleviate rat myocardial IR injury,which plays more effective cardioprotective effects than being used alone.
ABSTRACT
Objective To investigate the safety of tanshinone Ⅱ A sodium sulfonate in treatment of acute cerebral hemorrhage.Methods One hundred and seventy-two patients with acute cerebral hemorrhage were divided into tanshinone treatment group with 84 cases (tanshinone Ⅱ A sodium sulfonate + traditional treatment) and traditional treatment group with 88 cases (traditional treatment) according to the method of treatment.The safety (including neural function defect,adverse reactions,rebleeding rate and mortality and so on) were determined before treatment,and 2,4 weeks after treatment.Results The chinese stroke scale (CSS) scores in tanshinone treatment group after treatment of 2,4 weeks were lower than those in traditional treatment group [(13.2 ± 4.3) scores vs.(17.4 ± 5.6) scores,(8.7 ± 3.5) scores vs.(12.8 ± 4.6) scores],there were significant differences (t =5.498,6.556,P ＜ 0.01).The total effective rate in tanshinone treatment group was significantly higher than that in treatment group [83.3 ％ (70/84) vs.65.9 ％ (58/88)],there was significant difference (x2 =6.854,P ＜ 0.01).After treatment with tanshinone Ⅱ A sodium sulfonate in tanshinone treatment group,5 cases of mild skin rash,3 cases of nausea,vomiting,to turn for the better after symptomatic treatment.There was not other adverse reactions in the observation period.The mortality,rebleeding rate in tanshinone treatment group were lower than those in traditional treatment group,but there were no significant differences (P ＞ 0.05).Conclusions Early application of tanshinone Ⅱ A sulfonate treatment in acute cerebral hemorrhage has significantly efficacy,in the process of the clinical application is safe amd reliable.It is worthy of clinical popularization.