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Objective@#To analyze the improvement of executive function in children with attention deficit hyperactivity disorder (ADHD) aged 7-14 years with comprehensive intervention and drug therapy alone, to provide a basis for intervention research to improve ADHD.@*Methods@#A total of 80 children with ADHD treated in the Third People s Hospital of Ganzhou from January 2021 to June 2022 were randomly divided into intervention group and control group. The intervention group received drug and comprehensive intervention therapy, and sensory integration training once a week for 60 to 90 min each for 12 weeks, and conduct related training for caregivers and school teachers, the control group received only drug therapy. The changes of executive function were assessed by the stroop color word association test and the Wisconsin Card Sorting Test (WCST) after 12 weeks of intervention.@*Results@#After intervention, the results of the Stroop color word test in the intervention group (3.25±0.98, 4.92±1.40, 10.17±1.28) showed statistically significant differences ( t=12.94, 15.36, 26.34 , P <0.01) compared with those before intervention (6.47±1.92, 8.35±1.25, 16.55±1.57). There were also statistically significant differences ( t=6.76, 15.01, 16.15, P <0.01) in the control group ( 3.95 ±1.01, 5.45±1.15, 12.35±0.86) compared to those before intervention (6.17±1.87, 8.10±1.03, 16.02±1.38). Before intervention, the number of perseverative errors, non perseverative errors, and completed categories by WCST in the intervention group were (47.77±4.50, 35.50±2.37, 3.97±1.07), and in the control group were (46.45±7.34, 34.87±2.29, 3.70±1.11). After intervention, those of the intervention group and control group were (31.42±2.01, 24.75±2.05, 5.05±1.13) and (32.82±2.57, 25.55±1.04, 4.25±1.48), respectively. There were significant differences in the two groups before and after intervention ( t =21.93, 22.27 , -10.37; 10.84, 26.81, -6.90, P <0.01). After intervention, there were significant differences in the number of Stroop color word test errors, perseverative errors and non perseverative errors in WCST between the two groups ( t=-2.94, 2.29, -9.07, -2.35 , -2.06, P <0.05).@*Conclusion@#Through training for children and the therapy model of comprehensive intervention could significantly improve the executive function of children for a certain extent.
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Objective@#To investigate the prevalence of postmenopausal osteoporosis (PMOP) and analyze its influencing factors among women at ages of 50 to 59 years in Dali Bai Autonomous Prefecture, Yunnan Province, so as to provide insights into the prevention of PMOP among menopausal women.@*Methods@#Bai Ethnic menopausal women at ages of 50 to 59 years who received healthy examination at the Center of Healthy Examination, Dali Prefecture People's Hospital from June 2017 to May 2021 were selected as the study subjects, and subjects' demographic characteristics, living habits, history of diseases, family history of osteoporosis and history of parturition were collected using self-designed questionnaires. The height, body weight and bone density were measured, and fasting blood glucose, vitamin D3, blood lipids and liver functions were detected. The factors affecting the development of PMOP were identified using a multivariable logistic regression model.@*Results@#Totally 2 000 questionnaires were allocated, and 1 584 valid questionnaires were recovered, with an effective recovery rate of 79.20%. The respondents had a mean age of ( 56.22±2.61 ) years, and mean body mass index ( BMI ) of ( 24.62±2.35 ) kg/m2. There were 497 respondents ( 31.38% ) with a family history of osteoporosis, and the prevalence of PMOP was 20.64%. Multivariable logistic regression analysis identified age ( OR=1.135, 95%CI: 1.074-1.196 ), age of menarche ( OR=1.138, 95%CI: 1.059-1.217 ), duration of menopause (OR=1.425, 95%CI: 1.228-1.622), number of parturition ( >2, OR=5.036, 95%CI: 2.972-7.101 ), smoking ( OR=2.594, 95%CI: 1.767- 3.421 ), alcohol consumption ( OR=2.051, 95%CI: 1.503-2.598 ), family history of osteoporosis ( OR=2.540, 95%CI: 1.769-3.311 ), hypertension ( OR=1.492, 95%CI: 1.406-1.578 ), diabetes ( OR=1.774, 95%CI: 1.581-1.967 ), total cholesterol ( OR=1.483, 95%CI: 1.251-1.716 ), triacylglycerol ( OR=1.801, 95%CI: 1.576-2.026 ), low-density lipoprotein cholesterol ( OR=1.614, 95%CI: 1.498-1.731 ), fasting blood glucose ( OR=1.192, 95%CI: 1.077-1.307 ), BMI ( OR=0.934, 95%CI: 0.862-0.993 ), outdoor activity ( ≥1 time/week, OR: 0.413-0.549, 95%CI: 0.329-0.637 ), age of menopause ( OR=0.909, 95%CI: 0.841-0.977 ), daily intake of calcium ( ≥600 mg, OR: 0.493-0.644, 95%CI: 0.389-0.786 ), vitamin D3 level ( ≥20 ng/mL, OR: 0.604-0.719, 95%CI: 0.523-0.853 ) and high-density lipoprotein cholesterol ( OR=0.658, 95%CI: 0.550-0.767 ) as factors affecting the development of PMOP.@*Conclusions @#The prevalence of PMOP in Dali Bai Autonomous Prefecture is similar to the nationwide level in China, and old age, smoking, alcohol consumption, a family history of osteoporosis and high blood lipid levels may increase the risk of PMOP.
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Objective@#To analyze the effect and clinical value of the health management model of hypertension and chronic diseases developed by the health management center.@*Methods@#From June 2015 to June 2018, 550 patients with hypertension were selected in Taizhou Central Hospital and Taizhou Hospital.According to the management of chronic health point, the patients were divided into two groups, with 275 cases in each group.The patients before the chronic disease health management (from June 2015 to January 2017) were selected as control group, and the patients after implementing the health management of chronic disease (from February 2017 to June 2018) were selected as observation group.The standard blood pressure and blood fat were compared between the two groups.@*Results@#The compliance rate of blood pressure in the observation group was 61.09%, which was significantly higher than 34.91% in the control group, and the difference between the two groups was statistically significant(χ2=6.012, P<0.05). After intervention, the triglycerides [(1.47±0.21)mmol/L], total cholesterol[(4.61±0.51)mmol/L], and low-density lipoprotein [(2.76±0.22)mmol/L] in the observation group were significantly different with those in the control group (t=5.991, 6.398, 8.128, all P<0.05). After intervention, the body mass index [(24.32±1.88)kg/m3] and waist circumference [(83.26±6.03)cm] in the observation group were significantly different with those in the control group (t=5.928, 7.489, all P<0.05).@*Conclusion@#The model of health management for chronic diseases developed by the health management center can improve blood pressure and blood lipids of people who have physical examination, and provide effective health guidance services for patients with hypertension, which is worthy of further promotion in clinical practice.
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Objective To analyze the effect and clinical value of the health management model of hypertension and chronic diseases developed by the health management center.Methods From June 2015 to June 2018,550 patients with hypertension were selected in Taizhou Central Hospital and Taizhou Hospital .According to the management of chronic health point,the patients were divided into two groups ,with 275 cases in each group.The patients before the chronic disease health management (from June 2015 to January 2017) were selected as control group ,and the patients after implementing the health management of chronic disease (from February 2017 to June 2018) were selected as observation group.The standard blood pressure and blood fat were compared between the two groups .Results The compliance rate of blood pressure in the observation group was 61.09%,which was significantly higher than 34.91%in the control group,and the difference between the two groups was statistically significant (χ2 ＝6.012,P＜0.05). After intervention,the triglycerides [( 1.47 ±0.21) mmol／L], total cholesterol [( 4.61 ±0.51 ) mmol／L], and low－density lipoprotein [(2.76 ±0.22)mmol／L] in the observation group were significantly different with those in the control group ( t ＝5.991,6.398,8.128,all P＜0.05).After intervention,the body mass index [( 24.32 ± 1.88)kg／m3] and waist circumference [(83.26 ±6.03) cm] in the observation group were significantly different with those in the control group (t＝5.928,7.489,all P＜0.05).Conclusion The model of health management for chronic diseases developed by the health management center can improve blood pressure and blood lipids of people who have physical examination ,and provide effective health guidance services for patients with hypertension ,which is worthy of further promotion in clinical practice.
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Objective To study the clinical value of Time self -locking brackets in relieving the changes of dental arch and tooth morphology after maxillary dentition crowding .Methods From January 2015 to December 2015,80 patients with maxillary dentition in Rizhao Central Hospital were randomly divided into two groups according to the random number method ,with 40 cases in each group.The control group was treated with traditional MBT straight wire brackets (3M Company).The observation group was treated with Time self -locking brackets.And the width of the two groups was compared before and after the intervention.The treatment time and the incidence of complications during the treatment were observed in the two groups.Results After intervention,the width of canine and the length of long,middle bow in the observation group were significantly better than those in the control group ( t＝7.901, 29.820,15.652,all P＜0.05).The treatment time of the observation group was significantly shorter than that of the control group (t＝41.716,P＝0.000＜0.05).The incidence rate of bleeding ,swelling and loosening in the observa-tion group was significantly lower than that in the control group (χ2＝5.212,P＜0.05).The bracket shedding rate in the observation group was significantly lower than that in the control group ( χ2＝5.600,P＜0.05).Conclusion Time self-locking brackets is effective in the treatment of maxillary dentition crowding.The method has the advantages of high effect,simple clinical treatment ,less complication and low shedding rate during treatment.
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α-Amino acid ester acyltransferase (Aet) catalyzes the L-alanyl-L-glutamine forming reaction from L-alaine methylester hydrochloride and L-glutamine. In this study, the recombinant Escherichia coli saet-QC01 was used to express the α-amino acid acyltransferase, and its expression conditions were optimized. The recombinant protein was separated and purified by Ni-NTA affinity chromatography, and its enzymatic properties and catalytic applications were studied. The induction conditions suitable for enzyme production optimized were as follows: The temperature was 20 ℃, the induction stage (OD₆₀₀=2.0-2.5), IPTG concentration was 0.6 mmol/L, induction time was 12 h. The optimal reaction conditions of α-amino acid acyltransferase were 27 ℃, pH 8.5, it was most stable between pH 7.0 and 8.0 and relatively stable in an acidic environment, and low concentration of Co²⁺ or EDTA could promote the enzyme activity. Under optimal reaction conditions, 600 mmol/L of L-alaine methylester hydrochloride and 480 mmol/L of L-glutamine, the yield of L-alanyl-L-glutamine reached 78.2 g/L and productivity of 1.955 g/L/min, the conversion rate reached 75.0%. α-Amino acid ester acyltransferase has excellent acid-basei resistance, high catalytic efficiency. These characteristics suggest its application prospects in the industrial production.
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Dextranase can degrade dextran polymer into low molecular weight polysaccharide. Dextranase and its hydrolysates are widely used in food, medicine and chemical industries. Studies on dextranase progresses rapidly in recent years. We reviewed literature reports combined with our study about the progress of dextranase and its potential applications in industry. In addition, we addressed hot topics and emphasized on the current research about dextranase, existing problems in domesticstudies and the future research needs needs.
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Dextranase , Chemistry , Dextrans , Chemistry , Molecular Weight , PolymersABSTRACT
With the daily increase of living and working pressure in people,sub-health problem has been more and more predominant.How to guide people to throw of the attack of sub-health has been our major task.Through analyzing the distribution,type and attention degree of sub-health population,this article investigates points of science popularization for providing target-orientation science popularization knowledge.
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The importance of chitinases in the physiological and developmental processes of fungi and insects makes themselves and their inhibitors important targets for biological pesticides. A chitinase was isolated from Bombyx mori and purified to electrophoretic homogeneity by ammonium sulfate precipitation and Sephadex G-150 column chromatography. The molecular mass was estimated to be about 88 kDa by SDS-PAGE, while the K(m) was calculated to be 22.3 micromol/L. Moveover, the optimal reaction temperature was 45 degrees C, and the optimum pH was 6.0. The effect of metal ions and organic reagents on chitinase activity was investigated. The activity was enhanced by high concentration of Mn2+, while was strongly inhibited by Cu2+ and SDS. These results provide a basis for screening the chitinase-based biological pesticide.
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Animals , Bombyx , Chitinases , Metabolism , Enzyme Stability , Insect Proteins , Metabolism , TemperatureABSTRACT
We optimized the medium for recombinant dextransucrase expression in engineering strain Escherichia coli BL21 (DE3)/pET28-dexYG by an Orthogonal experiment. After the medium had been decided, we studied the effect of temperature, sucrose concentration and pH value on the yield. The results indicated that optimal conditions were adding IPTG of 0.25 mmol/L when OD600 reached 2.0 and cultivation lasted for 4 h at 25 degrees C. Under the selected medium and these conditions, the dextransucrase activity expressed by the engineering strain was high activity. Maximal activity reached 110.16 U/mL sucrose concentration effects the dextran yield grately. The results for dextransucrase expression would provide foundation for industrial application of dextransucrase.
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Culture Media , Culture Techniques , Methods , Dextrans , Escherichia coli , Genetics , Metabolism , Glucosyltransferases , Genetics , Recombinant Proteins , Genetics , Sucrose , MetabolismABSTRACT
Objective To compare on the influence of invigorating spleen and restoring virility to the SS level and SSR1 gene expression in brain. Method The spleen insufficiency model of rat was established by adopting bitter decline diarrhea, wild eating and overworking. SS level and SSR1 gene expression change of hypothalamus ventral kernel, hippocampi CA1 area and forehead corte were detected by immunohistochemistry and in situ hybridization techniques. Result In the model group, the immunoreaction masculine substance of SS and SSR1 mRNA, hypothalamus ventral kernel, hippocampi CA1 area and forehead cortex decreased obviously. The immunoreaction masculine substance of SS and SSR1 mRNA on above mentioned parts in the group of invigorating spleen and restoring virility increased distinctly. Conclusion In the model group, the SS level and SSR1 gene expression in brain to promoting study memory ability changed. Invigorating spleen and restoring virility may affect study memory ability by regulating the SS level and SSR1 gene expression.
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Objective To study the effect of lead on the calcium absorption and bone development in weanling rats. Methods Totally 80 weanling Wistar rats were equally divided into normal control (given with pure water and standard feed, including 1.15% calcium), lead group (given 1.0 g/L lead acetate water and standard feed), lead+low calcium group (1.0 g/L lead acetate water and feed including 0.69% calcium), and lead+high calcium group (1.0 g/L lead acetate water and feed including 1.72 % calcium). The development of rats was observed. Serum contents of osteocalcin and parathormone,and bone levels of lead and calcium in the femur were determined. The femur was examined with histological method. Another 5 Wistar rats received gastric irrigation of 10% lead acetate for 5 d, 5 more rats served as control, and then their absorption of calcium was detected with 45CaCl2. Results Lead and low calcium inhibited the development of rat remarkably, with the content of osteocalcin, the length and diameter of the femur decreased. High calcium antagonized these effects of lead. The absorption of calcium in rats was repressed by lead. Conclusion Bone depression by lead may be due to that lead inhibits the absorption of calcium in rats, and the supplement of calcium is helpful to minimize the repression.
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Objective: To study the effect of calcium on osteocalcin(OC) and parathyroid hormone (PTH) in rat exposed to lead. Method: Wistar rats were randomly divided into control group, lead group, low-calcium+lead group and high-calcium+lead group. The content of calcium and lead in bone, OC and PTH of rat with different treatments were detected. At the same time, RT-PCR analysis was used to determine the expression of OC and PTHr1. Results: Lead decreased the content of calcium in bone and OC. The expression of OC was inhibited, and the level of PTH and the expression of PTHr1 increased, which could be enhanced by low calcium treatment, but high calcium treatment could antagonize these effects of lead. Conclusion: Supplementation of calcium in diet could extenuate the toxicity of lead on bone in rat.