ABSTRACT
@#A qualitative analytical method of liquid chromatography coupled with mass spectrometry(LC-MSn)was developed for the identification of main constituents in Chrysanthemum morifolium ‘Fubaiju’. High-performance liquid chromatography(HPLC)was developed for the quantification of five active components, including chlorogenic acid(1), luteolin-7-O-β-D-glucopyranoside(2), luteolin-7-O-β-D-glucopyranuronide(3), 3, 5-Di-caffeoylquinic acid(4), and apigenin-7-O-β-D-glucopyranoside(5). A total of 22 compounds, including 13 flavonoids and 9 phenolic acids, were identified based on their retention behaviors, UV profiles and MS fragment information. Furthermore, a validation method with good linearity(r> 0. 999 9), precision, stability, repeatability and recovery was successfully applied for simultaneous determination of five major components in 10 batches of C. morifolium ‘Fubaiju’ by HPLC-UV method. The established method was proved to be a validation strategy for the quality evaluation of C. morifolium ‘Fubaiju’.
ABSTRACT
To evaluate the clinical therapeutic effect of limited bronchus granuloma caused by foreign body with medication in trachea, 6F tube was put in sick trachea under fibrobronchoscopy in 26 patients with bronchus granuloma due to foreign body, and 5ml of 2% lidocaine + 10ml of sterile Saline + 5mg dexamethasone +antibiotics (Amikacin0 2g or ceftriazone1 0g) were injected into sick trachea through 6F tube twice a day. 25 were cured, the cure rate was 96 2%,and the other one had to be operated; the course was 6～15d. Other 29 patients were treated with routine antibiotics intravenation+aerosol inhalation or medication in trachea under fibrobronchoscopy every other day, and 24 were cured, the cure rate was 82 8%; the other 5 had to be operated, the course was 6～32d. It is suggested that the former method has good effect in local anti inflammation, and can decrease the intravenous antibiotics dosage and side effect.