ABSTRACT
AIM: To explore the antipyretic mechanism of Qing Kai-Ling (QKL) injection on endotoxin (ET)-induced fever in rabbits. METHODS: Rabbit models of endotoxin (ET)-induced fever were duplicated. The rectal temperature was measured by digital thermograph. The cAMP and IL-1? content in the hypothalamus (HP), the cAMP content in the cerebrospinal fluid (CSF), and the arginine vasopressin (AVP) content in the ventral septal area (VSA) were determined by radioimmunoassay. RESULTS: ① QKL had significant antipyretic effect on ET-induced fever( P
ABSTRACT
AIM: The effects of Jiere Xingshen(JRXS) Injection on cAMP, IL-1 ? content in hypothalamus (HP) of endotoxin(ET)-induced feverish rabbits were studied. METHODS: The ET-induced fever model was established in rabbits and the cAMP content in hypothalamus (HP) and csf, IL-1 ? content in HP were determined by radioimmunoassay following intravenous infusion of JRXS. RESULTS: In ET group, the ?T [(0 40?0 11)℃], TRI 1(1 78?0 79), cAMP content in HP [(2 90?0 40) nmol/g], cAMP content in csf [(32 10?4 51) nmol/L)], IL-1 ? content in HP[(6 08?0 79) ng/g] were higher than that of NS and JRXS+ET group ( P
ABSTRACT
AIM: To investigate the mechanism of Qing-Kai-Ling(QKL) Injecta suppressing endotoxin(ET)-induced fever in rabbits.METHODS: ①The ET-induced fever model was established in rabbits. QKL injecta was administered intravenously, and the febrile response of rabbits was observed. The cAMP content in the hypothalamus (HP) and arginine vasopressin (AVP) content in the ventral septal area (VSA) were determined by radioimmunoassay.RESULTS: ①The maximal increment in body temperature (△T)[(1 68?0 46)℃],6 h thermal response index (TRI 6)(29 59?10 39), cAMP content in the HP[(2 90?0 40) nmol/g] and AVP content in the VSA[(47 32?3 77) ng/g] in the ET group were significantly higher than those in the normal saline (NS) group[(0 08?0 11)℃,-0.15?4 29,(1 05?0 37) nmol/g,(11 78?7 87) ng/g, respectively] and the QKL+ET group[(0 48?0 18)℃, (13.71?3.29), (1 37?0 23) nmol/g,(25 24?2 61) ng/g, respectively].②The positive correlation was observed between the cAMP content in the HP and the fluctuation of body temperature ( r=0.904,P