ABSTRACT
Objective Comparing the differences of gut microbiota between healthy women and URSA patients with the syndrome of liver depression and kidney deficiency,and the relationship between gut microbiota and age,abortion times,TCM syndrome score,and DASS-42 in URSA patients.Exploring the relationship between gut microbiota and clinical symptoms.Methods The observation group was 28 unpregnant URSA patients with the syndrome of liver depression and kidney deficiency,and the control group was 43 healthy unpregnant women.General data and stool samples were collected from two groups.Characterizing the composition of the fecal microbiota using bacterial 16S rRNA gene sequencing.The sequencing results were analyzed for diversity and species.TCM syndrome score and DASS-42 were collected from the URSA group,and the relationship between these scores and gut microbiota was analyzed.Results ①Beta diversity analysis shown that the gut microbiota of URSA patients with the syndrome of liver depression and kidney deficiency is different from that of healthy unpregnant women.②At the phylum level,Firmicutes were the most abundant in both groups.Compared with the normal group,the levels of Bacteroidetes and Proteobacteria in the URSA group were higher,and the levels of Actinobacteria were lower.At genus level,the dominant bacteria in URSA group are Faecalibacterium,Bacteroides,Megamonas,Bifidobacterium,Prevotella_9.③In the dominant bacteria of URSA group:Glucerabacter was positively correlated with age,Faecalibacterium was positively correlated with the number of abortions,Catenibacterium was negatively correlated with depression,anxiety,stress,DASS total score,Megasphaera was negatively correlated with depression,TCM syndrome score;In the dominant bacteria of normal group:Erysipelotrichaceae_UCG-003 was positively correlated with stress,the number of abortions,Bifidobacterium was negatively correlated with TCM syndrome score.Conclusion The gut microbiota composition was different between URSA patients with the syndrome of liver depression and kidney deficiency and healthy nonpregnant women,at the phylum and genus levels,the differences in gut microbiota between the two groups were observed,and the differential gut microbiota was associated with URSA patients′TCM syndrome score,suggesting that gut microbiota may be used as an indicator for the diagnosis and treatment of URSA with the syndrome of liver depression and kidney deficiency in the future,which is of clinical significance.