ABSTRACT
Neuron-glial antigen-2 (NG2) glia undergo proliferation and morphological changes following brain insults. Here, we show that NG2 glia is activated in a characteristic time- and layer-specific manner in the ischemia-vulnerable CA1 region of the rat hippocampus. Resting NG2 glia of the pyramidal cell layer (somatic region) shared morphological features with those of the neighboring dendritic stratum radiatum. During the postischemic period, reactive NG2 glia of the pyramidal cell layer exhibited shortened, scarcely branched processes, while those of the stratum radiatum had multiple branching processes with their arborization being almost indiscernible 7~14 days after reperfusion. Immunoelectron microscopy demonstrated that NG2 immunoreactivity was specifically associated with the plasma membrane and the adjacent extracellular matrix of NG2 glia in the stratum radiatum at 14 days. NG2 glia also exhibited differences in their numbers and proliferation profiles in the two examined hippocampal strata after ischemia. In addition, induced NG2 expression in activated microglia/macrophages exhibited a characteristic strata-dependent pattern in the ischemic CA1 hippocampus. NG2 induction was prominent in macrophage-like phenotypes which were predominantly localized in the pyramidal cell layer, compared with activated stellate microglial cells in the stratum radiatum. Thus, our data demonstrate that activation of NG2 glia and the induction of NG2 expression in activated microglia/macrophages occur in a distinct time- and layer-specific manner in the ischemic CA1 hippocampus. These characteristic profiles of reactive NG2 glia could be secondary to the degeneration processes occurring in the cell bodies or dendritic domains of hippocampal CA1 pyramidal neurons after ischemic insults.
ABSTRACT
Objective To explore the effect of ritodrine hydrochloride combined with magnesium sulfate in gestational diabetes mellitus pregnant women with threatened premature labor.Methods 74 gestational diabetes mellitus pregnant women with threatened premature labor were selected as the research subjects.According to the digital table,they were randomly divided into observation group and control group,37 cases in each group.The control group was treated with magnesium sulfate,the observation group was treated with ritodrine hydrochloride combined with magnesium sulfate.During treatment and after drug withdrawal 3 days,the fasting blood glucose levels and postprandial 2h blood glucose levels during pregnancy,prolonged time,complications and neonatal conditions in the two groups were recorded.Results The total effective rate of the observation group was 97.30％,which was significantly higher than 83.78％ of the control group,the difference was statistically significant between the two groups(u =2.336,P =0.019).The fasting blood glucose and 2h postprandial blood glucose levels were higher than before treatment in the two groups,but there were no statistically significant differences between the two groups (t =1.904,P =0.060;t =1.698,P =0.093).After stopping the drug,the fasting blood glucose and 2h postprandial blood glucose of the two groups were reduced to the pre-treatment levels,but there were no statistically significant differences between the two groups (t =0.953,P =0.343;t =0.749,P =0.456).The average gestation duration prolonged time of the observation group was (22.59 ± 2.84)d,which was significantly longer than that of the control group(t =11.737,P =0.000).The neonatal birth weight and Apgar score of the observation group were significantly higher than those of the control group (t =12.746,P =0.000;t =10.159,P =0.000).The neonatal survival rate of the observation group was 97.30％,which of the control group was 93.78％,the difference was statistically significant between the two groups (x2 =3.944,P =0.047).Conclusion Ritodrine hydrochloride combined with magnesium sulfate in the treatment of gestational diabetes mellitus pregnant women with threatened premature labor has good results,and can prolong pregnancy,and has less complications.
ABSTRACT
Objective To discuss application of double pipe for negative pressure drainage in reducing intra-abdominal infections after gastrointestinal operation.Methods 85 patients with intra-ahdominal drainage after gastrointestinal operation were randomly divided into the control group (43 patients) and the observation group(42 patients).The control group used traditional latex drainage,the observation group took the double cannula negative pressure continued drainage.The intra-abdominal infection and bacterial number distribution,patients drainage time,drainage volume and drainage tube occlusion were observed.Results 25 bacterial cultures were positive in two groups of patients.Compared between the two groups,the bacterial number detection rate were significant.31 pathogenic bacteria were detected in 25 specimens.Compared between these two groups,staphylococcus aureus,clearing time of drainage,drawing tube time after drainage liquid being clear,the total catheterization time,average daily drainage volume were all significantly different.Conchrsious Double pipe negative pressure drainage for postoperative abdominal drainage can effectively reduce abdominal infection,shorten drainage time,and its effect is very good.