Antimicrobial and antioxidant activity of Panax ginseng and Hedysarum neglectum root crop extracts / Atividade antimicrobiana e antioxidante de extratos de raízes de Panax ginseng e Hedysarum neglectum

In order to ensure the timely and uninterrupted supply of medicinal plant raw materials, the methods of cultivation of plant cell cultures, namely, the production of plant root cultures, are relevant. In this paper, the geroprotective potential of Hedysarum neglectum Ledeb and Panax ginseng C. A. Mey root cultures is studied. They were cultured under in vitro conditions by transforming the rhizome (H. neglectum) and seed seedlings (P. ginseng) with Agrobacterium rhizogenes 15834 Swiss. To identify the geroprotective potential, the antimicrobial disc-diffusion method and the antioxidant activity were analyzed by titration of KMnO4 extracts of plant root cultures. The qualitative and quantitative composition was analyzed using high-performance liquid chromatography, thin-layer chromatography, and gas chromatography with mass spectrometry. In the course of the work, the presence of antimicrobial and antioxidant activity of plant root culture extracts was established. Biologically active substances contained in extracts of Hedysarum neglectum Ledeb root crops and Panax ginseng C. A. Mey are characterized by geroprotective potential, so they can act as a source of natural antioxidants in the functional nutrition of the geroprotective orientation.

Para garantir o abastecimento em tempo e ininterrupto de matérias-primas de plantas medicinais, são relevantes os métodos de cultivo de culturas de células vegetais, nomeadamente a produção de culturas de raízes vegetais. Neste trabalho, foi estudado o potencial geroprotetor de culturas de raízes de Hedysarum neglectum Ledeb e Panax ginseng C. A. Mey. Eles foram cultivados em condições in vitro pela transformação do rizoma (H. neglectum) e mudas de sementes (P. ginseng) com Agrobacterium rhizogenes 15834 Swiss. Para identificar o potencial geroprotetor, o método antimicrobiano de difusão em disco e a atividade antioxidante foram analisados por titulação de extratos de KMnO4 de raízes de plantas. A composição qualitativa e quantitativa foi analisada por cromatografia líquida de alta eficiência, cromatografia em camada delgada e cromatografia gasosa com espectrometria de massa. No decorrer do trabalho, foi constatada a presença de atividade antimicrobiana e antioxidante dos extratos de raízes de plantas. Substâncias biologicamente ativas contidas em extratos de raízes de H. neglectum Ledeb e P. ginseng C. A. Mey são caracterizadas pelo potencial geroprotetor, podendo atuar como fonte de antioxidantes naturais na nutrição funcional da orientação geroprotetora.

Antimicrobial and antioxidant activity of Panax ginseng and Hedysarum neglectum root crop extracts

Abstract In order to ensure the timely and uninterrupted supply of medicinal plant raw materials, the methods of cultivation of plant cell cultures, namely, the production of plant root cultures, are relevant. In this paper, the geroprotective potential of Hedysarum neglectum Ledeb and Panax ginseng C. A. Mey root cultures is studied. They were cultured under in vitro conditions by transforming the rhizome (H. neglectum) and seed seedlings (P. ginseng) with Agrobacterium rhizogenes 15834 Swiss. To identify the geroprotective potential, the antimicrobial disc-diffusion method and the antioxidant activity were analyzed by titration of KMnO4 extracts of plant root cultures. The qualitative and quantitative composition was analyzed using high-performance liquid chromatography, thin-layer chromatography, and gas chromatography with mass spectrometry. In the course of the work, the presence of antimicrobial and antioxidant activity of plant root culture extracts was established. Biologically active substances contained in extracts of Hedysarum neglectum Ledeb root crops and Panax ginseng C. A. Mey are characterized by geroprotective potential, so they can act as a source of natural antioxidants in the functional nutrition of the geroprotective orientation.

Resumo Para garantir o abastecimento em tempo e ininterrupto de matérias-primas de plantas medicinais, são relevantes os métodos de cultivo de culturas de células vegetais, nomeadamente a produção de culturas de raízes vegetais. Neste trabalho, foi estudado o potencial geroprotetor de culturas de raízes de Hedysarum neglectum Ledeb e Panax ginseng C. A. Mey. Eles foram cultivados em condições in vitro pela transformação do rizoma (H. neglectum) e mudas de sementes (P. ginseng) com Agrobacterium rhizogenes 15834 Swiss. Para identificar o potencial geroprotetor, o método antimicrobiano de difusão em disco e a atividade antioxidante foram analisados por titulação de extratos de KMnO4 de raízes de plantas. A composição qualitativa e quantitativa foi analisada por cromatografia líquida de alta eficiência, cromatografia em camada delgada e cromatografia gasosa com espectrometria de massa. No decorrer do trabalho, foi constatada a presença de atividade antimicrobiana e antioxidante dos extratos de raízes de plantas. Substâncias biologicamente ativas contidas em extratos de raízes de H. neglectum Ledeb e P. ginseng C. A. Mey são caracterizadas pelo potencial geroprotetor, podendo atuar como fonte de antioxidantes naturais na nutrição funcional da orientação geroprotetora.

Pratique anesthésique pour adénomectomies hypophysaires par voie endonasale à Abidjan, Côte d'Ivoire / Anesthesia for endonasal pituitary adenomectomies in Abidjan, Ivory Coast

L'objectif de l'étude est d'évaluer la pratique anesthésique au cours des adénomectomies hypophysaires par voie endo nasale à Abidjan. Méthode : Il s'agissait d'une étude rétrospective à visée analytique et descriptive, menée entre le 01 janvier 2010 et le 31 décembre 2020 et portant sur les patients admis au bloc opératoire pour une adénomectomie hypophysaire. Les caractéristiques sociodémographiques, cliniques, anesthésiques, évolutives et pronostiques ont été étudiées. Résultats : Nous avons recensé 102 patients. L'âge moyen était de 44,72±12,14 ans. Le sex ratio était de 1. Le délai de diagnostic était d'un an pour 46% (n = 47) des patients. L'hypertension artérielle était l'antécédent le plus retrouvé. Les principaux motifs de consultation étaient les céphalées et les troubles de l'acuité visuelle. Le syndrome d'hypertension intracrânien a été observé chez 67,6% (n= 69) des patients. Tous les patients ont bénéficié d'une consultation pré anesthésique. Cinquante-huit pourcent (n=59) des patients ont été classés ASA I selon la société américaine d'anesthésie. L'anesthésie générale était le seul schéma anesthésique. La durée de l'anesthésie était de plus de trois heures chez 57% (n=58) des patients et le réveil sur table a concerné 88,2% (n=90) des patients. La mortalité était de 3,9%. La durée de l'anesthésie supérieure à 6 heures (p= 0,0012 ; OR= 55,8 [4,88-637,33]) et la perte sanguine supérieure à 1000 ml (p = 0,0228 ; OR=18,6 2,152- 160,747]) ont constitué des facteurs de mauvais pronostic (p<0,05). Conclusion: La réduction de la létalité au cours de l'anesthésie pour adénomes hypophysaires passe par la lutte contre les facteurs de mauvais pronostics

An annual retrospective analysis of adverse drug reactions reported at adverse drug reaction monitoring center, Nalgonda

Background: Adverse drug reaction (ADR) monitoring is important for risk benefit analysis and for patient’s safety. ADR monitoring helps in maintaining the database which is specific to Indian population. Aims and Objectives: This study aims to analyze the pattern of ADRs, drug implicated, organ system affected, severity of ADRs, and their causality assessment. Materials and Methods: It was a retrospective analysis of all the ADRs reported to ADR monitoring center, Nalgonda, from January 2019 to December 2019. Prior ethics committee approval was obtained. ADRs were collected using suspected ADR forms provided by National Coordination Center-Pharmacovigilance Programme of India (NCC PVPI). All the ADRs were reported to NCC PVPI through VigiFlow software. Results: A total of 273 ADRs were reported of which 59% were female and 41% were male. According to the age group, 30–39 years were the most common age group affected about 26%. The most common drug class implicated for ADRs was antimicrobials. Skin was the most common system affected. Most of the ADRs belonged to probable category according to the WHO causality assessment scale. Only 9 (3%) ADRs were of serious nature. Conclusion: Pharmacovigilance is important for identification of ADRs due to drugs. Still, lot of awareness about pharmacovigilance needs to be created among health-care professionals to improve the reporting of ADRs. This, in turn, will improve the drug safety among patients

Probiotic supplementation affects IGF-1 and leptin levels in Nile tilapia hepatopancreatic tissue / Suplementação com probiótico afeta os níveis de IGF-1 e leptina no tecido hepatopancreático de tilápias-do-nilo

This work aimed to assess the effect of the probiotic strain, Lactobacillus plantarum, on the levels of leptin, IGF-1 and their receptors on the hepatopancreatic tissues of Nile tilapia (Oreochromis niloticus) and then correlate fish growth performance and gut microbiological parameters. Fish juveniles (±23g) were reared in a recirculation system with constant aeration and temperature (25°C). They were distributed into six polyethylene tanks (45L) and fed twice a day at 5% of the tank biomass with the respective diets: control (commercial diet without probiotic) and supplemented with L. plantarum inoculum (1 x 108 CFU mL-1), both in triplicate. After 30 days of feeding, L. plantarum-fed fishes showed greater weekly growth rate, final weight, and feed conversion rate, in addition to higher count of lactic-acid bacteria and lower count of pathogenic bacteria in the intestinal tract, when compared to the control group. The immunostaining intensity for IGF-1 and leptin hormones was lower after L. plantarum supplementation than in the control group, with no change in the level for receptors. This reduction could implicate important changes in fish metabolism and homeostasis.(AU)

O presente trabalho teve como objetivo avaliar o efeito da cepa probiótica Lactobacillus plantarum sobre os níveis de leptina, IGF-1 e seus receptores no tecido hepatopancreático de tilápia-do-nilo (Oreochromis niloticus) e correlacionar com o desempenho zootécnico e os parâmetros microbiológicos intestinais dos peixes. Juvenis de tilápia-do-nilo (±23g) foram distribuídos em seis tanques de polietileno (45L) conectados a um sistema de recirculação, com aeração e temperatura constantes (25°C). Os peixes foram alimentados duas vezes ao dia, a 5% da biomassa do tanque, com as respectivas dietas: controle (dieta comercial sem probiótico) e suplementada com L. plantarum (1 x 108 UFC mL-1), ambas em triplicata. Após 30 dias de cultivo, os peixes alimentados com L. plantarum apresentaram maiores ganho de peso semanal, peso final e conversão alimentar, bem como maior contagem de bactérias ácido-láticas e menor contagem de bactérias patogênicas no trato intestinal das tilápias alimentadas com dieta probiótica, em comparação ao grupo controle. A intensidade da imunomarcação para os hormônios IGF-1 e leptina foi menor com a suplementação de L. plantarum do que no grupo controle, sem alterar os níveis de seus receptores. Essa redução pode implicar mudanças importantes no metabolismo e na homeostase dos peixes.(AU)

Preoperative Diagnosis of Upper Gastrointestinal Leiomyoma by Endoscopic Ultrasound-guided Fine Needle Aspiration

Aims: To evaluate the role of endoscopic ultrasonography-guided fine needle aspiration (EUS-FNA) with using immunohistochemical analysis in the preoperative diagnosis of upper gastrointestinal leiomyoma.StudyDesign:This was ’prospective’observational study.Place and Duration of Study: Department of surgery No1, Vinnytsia National Pirogov Medical University,Vinnytsia, Ukraine; betweenSeptember 2016 and February 2019. Methodology:Sixteen prospectively studies have been performed using endoscopic ultrasonography-guided fine needle aspiration (EUS-FNA) in patients with submucosal hypoechoic tumors (according to the results of previous gastroduodenoscopy) with continuity to proper muscle layer suspected asleiomyoma of upper gastrointestinal tract. All cases for the final diagnosis underwent surgery (n = 16). Additionally, immunophenotyping of specimens obtained by EUS-FNA and surgical resection specimens have been compared. Results:The puncture has been performed in all patients without any anatomical problems. The collection rate of adequate specimens from the GI tract subepithelial hypoechoic tumor with continuity to proper muscle layer was 87, 5%. The diagnostic rate for the tumor less than 2 cm, 2 to 4cm, and 4 cm or more were 77, 8%, 100% and 100% respectively. In 16 surgically resected cases, the sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of EUS-FNA using immunohistochemical analysis of leiomyoma were 100%; 83,3%; 90,9%; 100% and 93,75% respectively. No major complications were encountered.Conclusion:EUS-FNA with immunohistochemical analysis is a safe and accurate method in the preoperative diagnosis of gastrointestinal leiomyoma. It should be taken into consideration in decision making, especially in early diagnosis following minimal invasive surgeryfor gastrointestinal leiomyoma.

BIOFLOC: sustainable alternative for water use in fish culture / BIOFLOCO: alternativa sustentável para uso de água na piscicultura

Este estudo objetivou avaliar o desempenho do crescimento e o consumo de água de alevinos de tilápia-do-nilo (Oreochromis niloticus) criados em tecnologia de Biofloco (BFT) e no sistema tradicional. Alevinos de tilápia-do-nilo foram divididos em dois tratamentos: peixes criados em BFT e em sistema convencional. Os peixes foram alimentados três vezes ao dia, com 6% da biomassa corporal. O oxigênio dissolvido e a temperatura foram mensurados duas vezes ao dia; o total de sólidos suspensos, o pH, a alcalinidade, a amônia, o nitrito, o nitrato e o fosfato foram monitorados duas vezes por semana. Após 42 dias, avaliou-se o desempenho do crescimento da tilápia-do-nilo. A temperatura (25,1 e 21,7°C), a alcalinidade (37,1 e 16,9mg L -1 ), o nitrito (48,4 e 0,0mg L -1 ) e o nitrato (134,3 e 0,0mg L -1 ) foram maiores no BFT do que no sistema convencional. A sobrevivência (95,80 e 100,00%), a conversão alimentar (1,00 e 1,39), a conversão alimentar de todos os insumos (1,14 e 1,39) e o volume de água para produzir 1,0 T de peixe (128,21 e 23.934,42m³) foram menores, assim como a proteína, a eficiência (2,24±0,21 e 1,58±0,31) e a produtividade (7,77±1,39 e 0,61±0,07kg.m -3 ) foram maiores para o BFT do que para o sistema convencional. A tilápia-do-nilo criada em BFT apresenta dados produtivos iguais e/ou melhores que os obtidos em sistemas tradicionais, consumindo menos volume de água.(AU)

A comprehensive study on jaundice in pregnancy with emphasis on fetomaternal outcome

Background: Jaundice affects a small percentage (1-4 per 1000) of pregnant women, yet it is an important medical disorder especially in developing countries like India. Jaundice in pregnancy carries adverse outcomes for both the fetus and the mother. It accounts for 60% perinatal and 14% of maternal deaths. The aim of the study is to know the incidence of jaundice, to evaluate the causes of jaundice and to know the effect of jaundice during pregnancy on maternal and fetal outcome. Materials and methods: All pregnant women with jaundice admitted in the Department of Obstetrics and Gynecology, King George Hospital, Visakhapatnam between September 2015 and August 2017 were taken up for study. Results: The incidence of jaundice was 7.22 per 1000 deliveries. Since 92.54% of patients were between 20-35 years of age, maximum number of cases was Primi gravidae. The most common cause of jaundice was HELLP syndrome, hepatitis being the second most common cause. Maternal mortality was 12.74%, the perinatal mortality was 35.71%, prematurity being the commonest cause. Conclusions: Jaundice in pregnancy has adverse fetomaternal outcome. It should be managed as a team in collaboration of obstetrician, physician, gastroenterologist, anesthetist and neonatologist. Improvement in health education, regular antenatal check-ups and early referrals result in early diagnosis and treatment of jaundice during pregnancy thus reducing maternal and fetal morbidity and mortality.

Thoracic Posture and Mobility in Mechanical Neck Pain Population: A Review of the Literature

Neck pain is a common condition with several proposed biomechanical contributing factors. Thoracic spine dysfunction is hypothesized as one of the predisposing factors, which necessitates the need to explore the contribution of thoracic posture and mobility toward neck pain. Accordingly, the present work aimed to review the existing literature investigating the presence of thoracic spine dysfunction in individuals with neck pain. A literature search was conducted in the three electronic databases of PubMed, CINAHL, and Web of Science. Studies published between 1990 and 2017 were considered. After reviewing the abstracts, two authors independently scrutinized the full-text documents for their relevance. The initial search yielded 2,167 articles, of which nine studies involving comparisons of neck pain patients and healthy controls were identified for the review. Increased thoracic kyphosis was positively correlated with the presence of forward head posture but not uniformly associated with neck pain intensity and disability. Thoracic mobility was reduced in the neck pain population, and the role of thoracic kyphosis as a risk factor for pain development could not be confirmed. Thus, an association exists between thoracic kyphosis and postural alteration in the cervical spine. The review favors the inclusion of thoracic spine assessment and treatment in mechanical neck pain patients. Further studies are needed to investigate the cause-effect relationship between thoracic posture and cervical dysfunction.

Immediate impact of successful percutaneous balloon mitral valvuloplasty on right and left ventricular functions: An echocardiographic study using load independent tissue velocity imaging indices

Background The impact of successful percutaneous balloon mitral valvuloplasty (PBMV) on left ventricular (LV) function has been a controversial subject. This study aimed to determine the immediate impact of PBMV on biventricular function using recent Tissue Velocity Imaging (TVI) derived load-independent indices. Methods and results A total of 30 patients with severe mitral stenosis (MS) who underwent PBMV at a tertiary center of India from August 2012 to December 2013 were included in the study. Thirty age-matched and gender-matched healthy controls were also enrolled. Out of 30 patients, 27(90%) were female. Mean mitral valve area (MVA) of patients before and after PBMV was 0.78 and 1.82 cm2 (p < 0.001), respectively. All TVI-derived LV and RV basal systolic (IVCV, Sm and the relatively load independent IVA) and diastolic velocities (Em, Em/Am) were significantly decreased in patients with MS compared to controls (p < 0.001 for all) which improved significantly after PBMV (6.4 ± 0.7 vs 11 ± 1.6; 5.8 ± 0.7 vs 9.9 ± 1.6; 1.5 ± 0.3 vs 4.2 ± 0.6; 6.4 ± 0.6 vs 13.1 ± 2.1; 0.7 ± 0.1 vs 1.7 ± 0.2 for mitral annulus respectively, p < 0.001 for all). Increment in MVA positively correlated with Tricuspid annular plane systolic excursion (TAPSE) and tricuspid annular Sm and isovolumic contraction velocity (IVCV) and inversely with left atrium (LA) size and Pulmonary arterial systolic pressure (PASP) (p = 0.01 for LA size; p < 0.001 for others) while no such correlation was found with mitral annulus isovolumic acceleration (IVA) (r = −0.078; p = 0.679). Conclusion The improved right ventricular (RV) function appears to be predominantly due to afterload reduction, while that of LV appears to be more due to the acute relief of mechanical restraint.

Mammographie et difficultés diagnostiqués des affections mammaires au Centre hospitalier universitaire Yalgado Ouédraogo (Chuyo) de Ouagadougou

L'objectif de cette étude était d'évaluer les difficultés liées au diagnostic mammographique des affections mammaires au Chu Yalgado ouédraogo de ouagadougou. il s'est agi d'une étude transversale descriptive menée du 1er janvier 2007 au 30 juin 2010 et qui a concerné 213 dossiers de patients ayant consulté pour une affection mammaire. nous avons recensé 213 patients dont les dossiers médicaux ont été retrouvés avec 95,4 % de femmes et 4,6 % d'hommes soit un sexe ratio de 0,05. la moyenne d'âge des patients était de 41,5 ans et la tranche d'âge de 43 à 52 ans était la plus représentée. la mammographie a pu être réalisée seulement par 44 patients soit 20,66 % du fait principalement du coût exorbitant de l'examen pour les populations majoritairement démunies, de la faible disponibilité de cette modalité d'exploration et de sa faible prescription. les comptes rendus d'examen étaient disponibles chez 27 patients (soit 61,3 %). des anomalies ont été retrouvées chez 73.1 % de ces patients, beaucoup plus chez les femmes (95 %) que chez les hommes (5 %). les lésions bénignes étaient l'apanage des tranches d'âge de 13 à 22 ans (68 %) et celles suspectes de malignité celui des patients plus âgés, de 43 à 52 ans (91 %). les principales lésions étaient représentées par les opacités (46,1 %) et les microcalcifications associées aux opacités (11.5 %). on notait une prédominance des lésions de type aCR 2 (22,2 %). la mammographie est l'examen de première intention dans le bilan sénologique mais elle reste relativement peu utilisée dans notre contexte

Management of severe sepsis and septic shock in pregnancy to improve the survival

Background: The thymus is a central lymphoid organ that plays a vital role in the development and maturation of the immune system during childhood, the thymus appears as a bilobed triangular structure located in the anterior mediastinum. Aim and objectives: To provide radiologists a comprehensive understanding of Recognition of the variable appearance of thymic lesions and evaluation of thymic lesions on different radiological modalities like X-Rays and CT-scan for early diagnosis as well as management. Materials and methods: 10 cases of either strong suspicion or symptoms related to thymic lesion were evaluated who came to Dhiraj Hospital with different radiological modalities (X-ray, CT-scan). Results: Out of total no. of 10 patients who were diagnosed and evaluated with thymic lesion on Xrays and CT-scan are: Thymic Hyperplasia, Thymic Cyst, Thymic Lymphoma, Thymoma, Benign Teratoma, Malignant Teratoma. Conclusion: CT Imaging remains the ideal scanning modality to evaluate Radiologists play a major role in differentiating normal thymus from its variants, various thymic lesions and tumor. But common associated radiological modality used along with CT scan done is X-ray.

Effective Position of Closed Suction Drain in Inguinal Hernia Surgery: A Retrospective Study.

Background: The aim of this study was to assess the efficacy of closed suction drains in inguinal hernia surgery inserted up to the base of the scrotum as compared to those in which the drain is limited to the inguinal region. Methods: Data of all lichtenstein hernioplasty from January 2012 to December 2014 in M. S. Ramaiah Medical Hospital were collected retrospectively. A total of 992 patients underwent hernia repair and only 133 patients were subjected to closed suction drain. In 133 patients data were collected regarding the type of presentation, age, gender, presence of coexisting diseases, type of hernia, type of anesthesia, postoperative general complications, data was collected regarding the presentation of the hernia, type of sac encountered, amount of dissection, location of the inserted drain, complications, and length of hospital stay. These patients were followed up for 6 months. Local wound complications, duration of operation, and length of hospitalization, recurrence and mortality were compared between the groups of patients with drains versus without drains. Results: Average amount of drain fluid was higher (58.5 ml) in the group where the drain was inserted to the bottom of the scrotum as compared to (22.4 ml) the group with the drain limited to the inguinal region. Furthermore, there was significant difference in the incidence of scrotal edema in the two groups (6 vs. 23). Hence, by positioning the drain to reach the bottom of the scrotum; it will lead to a better drainage of the collection and in turn lead to fewer associated complications such as scrotal hematomas, infections, and scrotal edema. Conclusion: In our study, placement of drain extending into the bottom of scrotum significantly reduced scrotal edemas a result alleviates immediate post-operative patient anxiety and morbidity in the reduction of scrotal size to normal. However, it requires a larger series to confirm our early observations.

Impacto de la frecuencia y la gravedad de la rinitis sobre la función pulmonar en niños y adolescentes sin asma / Impact of frequency and severity of rhinitis on lung function in children and adolescents without asthma

Introducción. Se han observado alteraciones espirométricas en pacientes con rinitis, sin asma, proporcionales al grado clínico de la rinitis establecido por la guía Allergic Rhinitis and its Impact on Asthma (ARIA). Recientemente se ha validado una nueva clasificación de la rinitis como ARIA modificada (ARIAm), que disocia la forma moderada de la grave. Se desconoce el impacto de la rinitis sobre la función pulmonar considerando su frecuencia y gravedad por separado. Objetivos. Determinar las posibles alteraciones de la función pulmonar y su potencial reversibilidad al broncodilatador en niños y adolescentes con rinitis alérgica y no alérgica sin asma, de acuerdo a su frecuencia (intermitente y persistente) y gravedad (leve, moderada y grave) establecida según ARIAm. Población y métodos. Estudio observacional, analítico de corte transversal, que incluyó pacientes de ambos sexos, entre 5 y 18 años, con rinitis alérgica y no alérgica, sin asma. Se realizó espirometría por curva flujo-volumen y se analizó la existencia de anormalidades en la función pulmonar y la respuesta broncodilatadora en relación con el grado de rinitis por ARIAm ajustando un modelo logístico. Se consideró un valor de p<0,05 para establecer significación estadística. Resultados. Se incluyeron 189 pacientes. En 42 (22,2%) se observó al menos un parámetro alterado en la función pulmonar. Los pacientes con rinitis persistente exhibieron mayor afectación de la función pulmonar que los de grado intermitente (p=0,0257). El defecto funcional fue más frecuente en las formas grave y moderada que en el grado leve (p=0,0052) y fue independiente de la condición de atopia (p=0,1574 para frecuencia y p =0,5378 para gravedad). No se encontró diferencia en la reversibilidad al broncodilatador entre los grupos (p=0,1859 y p=0,0575, respectivamente). Conclusiones. La alteración de la función pulmonar se asoció con rinitis persistente. Fue más prevalente en las formas moderada y grave pero no existieron diferencias significativas entre ambas. El defecto funcional se demostró tanto en rinitis alérgica como no alérgica. (AU)

Background: Subclinical spirometric abnormalities have been observed in patients with rhinitis without asthma, proportional to the clinical grade established by Allergic Rhinitis and Its Impact on Asthma (ARIA) guideline. Recently has been validated a new criteria of rhinitis classification designed as ARIA modified (ARIAm), which allow to discrimination moderate to severe grades. Rhinitis impact on lung function according to frequency and severity considering separately is unknown. Objectives: To investigate possible subclinical alterations in lung function and bronchodilator reversibility in children and adolescents with allergic and nonallergic rhinitis without asthma, according to the frequency and severity criteria of rhinitis classified by ARIAm. Methods: In an observational cross sectional study we included children and adolescents aged 5 to 18 years with symptoms of allergic and nonallergic rhinitis without asthma. Spirometry was performed by flow-volume curve and we analyzed the abnormalities in respiratory function and bronchodilator response in relation to clinical grade of rhinitis by ARIAm using an adjusted logistic model. A p value <0.05 was considered statistically significant. Results: We studied 189 patients; 42 (22.2%) had some spirometric abnormalities. Patients with persistent rhinitis had greater impairment of lung function compared to intermittently grade (p = 0.0257). The functional defect was more frequent in rhinitis severe and moderate than mild grade (p = 0.0052) and was independent of atopy status both frequency (p = 0.1574) and severity (p=0.5378). There was no difference in bronchodilator reversibility between groups (p = 0.1859 and p = 0.0575 respectively). Conclusion: Impaired lung function was associated with persistent rhinitis. It was more prevalent in moderate and severe rhinitis, but there wasn't a significant difference between them. The functional defect was demonstrated in both allergic and nonallergic rhinitis.(AU)

Same day sputum smear microscopy approach with modified ZN staining for the diagnosis of pulmonary tuberculosis in a microscopy centre at Rajahmundry.

Background: Sputum smear microscopy is the main‑stay in the diagnosis of pulmonary tuberculosis in many developing countries. To overcome the drop outs, same day diagnosis is ideal. Materials and Methods: In the current study, two spot sputum samples (SS2 approach) are collected within a gap of one hour (same day sputum smear microscopy) in addition to the standard spot morning (SM) approach. The smears were stained with standard Ziehl Neelsen (ZN) and modified ZN staining techniques. Results: Out of 1537 patients, sputum smear positivity (SSP) was 9.43% (146 patients) in SM approach with standard ZN staining. Smear positivity was increased to 9.8% (151 patients) with modified ZN staining. For SS2 approach, SSP was 9.37% (144 patients) and 9.8% (151 patients) with standard and modified ZN staining procedures, respectively. Conclusions: Diagnosis of lung tuberculosis is possible with two spot sputum samples with modified ZN staining.

Ascites in CML: a rare extramedullary manifestation.

Ascites in chronic myeloid leukemia (CML) as an extramedullary manifestation is rarely reported in the literature. A young 23 year old female of chronic myeloid leukemia presenting with extramedullary disease as massive ascites. All stages of granulocytes and a few blasts similar to peripheral blood smear was present in ascitic fluid. Based on clinical symptoms and signs, bone marrow examination, ascitic fluid cytology and ultrasonography of abdomen she was diagnosed as a case of Chronic myeloid leukemia in chronic phase with extramedullary disease as massive ascites. After starting treatment with Imatinib mesylate favourable response was observed.

Abamectin: an uncommon but potentially fatal cause of pesticide poisoning.

Human intoxication with abamectin is not frequently reported. It is an uncommon but potentially fatal cause of pesticide poisoning. In contrast to common organophosphate poisoning the toxic effects of avermectin in humans are not clearly defined. Ingestion of a large dose of avermectin may be associated with life-threatening complications. The therapy for avermectin poisoning is mainly symptomatic and supportive. The prognosis of patients with avermectin poisoning is likely to be favorable unless they are complicated by severe hypotension or aspiration. We hereby report a case with abamectin poisoning with neurological toxicity and respiratory failure which responded to supportive line of therapy.

Two Way Relation of Diabetes Medllitus And Periodontits - A Review.

The association between diabetes and periodontium is complex. Diabetes is a well established risk factor for periodontitis. Periodontitis infection complicates the severity of disease, and the degree of metabolic control of disease. Diabetes is a disease of metabolic dysregulation, primarily of carbohydrate metabolism, characterised by hyperglycemia that results from defects in insulin secretion, impaired insulin action or both. The major efforts should be directed at the prevention of periodontitis in patients at risk of developing diabetes. This proposed dual pathway of tissue destruction suggests that control of chronic periodontal infection is essential for achieving long-term control of diabetes mellitus. This dual relationship of the control of tissue destruction in chronic periodontal infection is essential also for the long term control of diabetes mellitus.

Free Gingival Graft In The Treatment of Isolated Gingival Recessions : A Case Report.

Gingival recession is an intriguing and complex phenomenon. Recession frequently disturbs patients because of sensitivity and esthetics. Many surgical techniques have been introduced to treat gingival recession, including those involving connective tissue grafting, various flap designs, orthodontics, and guided tissue regeneration. This article describes a different clinical approach to treat gingival recession with emphasis on techniques that show promising results and root coverage.