ABSTRACT
The twenty female Balb/c mice [6-8 week-old, 14 +/- 2 gr weights] were divided to two control and exercise groups. After orientation in the environment, estrogen-receptor dependent breast cancer cells [MC4-L2] were injected to them and exercise group performed endurance exercise for 6 weeks. After appearance of the tumor, the length and width of the tumor were measured by a digital caliper once a week. Finally, the mice were sacrificed; tumor tissue was removed and immediately frozen and kept in -70°C. Tumor sample was homogenized and levels of IL-10 were measured and quantified using ELISA method. There was significant increase on level of IL-10 in exercise group than control group [P>0.05]. Tumor volume reduced in exercise group than control group. The results showed that the amount of IL-10 significantly increased in the exercise group compared to control group Also reduction in tumor volume observed in the exercise group compared to control group. Increased levels of IL-10 was in parallel to reduction in tumor volume, which shows that exercise lead to reduction tumor growth through increased levels of this cytokine. Angiogenesis is a prerequisite for continued growth and spread of tumors, therefore, increased levels of IL-10 that is an angiogenesis cytokine in the exercise group compared to the control group shows exercise can inhibit from tumor growth by modulating cytokine profile within the tumor microenvironment