ABSTRACT
Alpha toxin is found to be produced by all types of Clostridium perfringens. The genes encoding alpha toxin from the available five types of Clostridium perfringens [A I chicken strain], A [rabbit strain], B, C and D were PCR amplified using specific primers and the PCR products were examined on 1.5% [w/v] agarose gel and demonstrated the same bands comparable to the published alpha toxin gene. The PCR products of a toxin gene from the five types were separately sequenced and aligned with all published a toxin genes of Clostridium perfringens. Identities among all studied alpha toxin gene sequences and with the published ones were nearly 96-98%. There are no any significant differences among these nucleotide sequences. It is concluded that a toxin gem; sequences among different types of Clostridium perfringens are similar and highly conserved