Using some recent techniques in diagnosis of some zoonotic bacterial diseases transmitted through milk

A total of 200 different samples [100 raw milk, 50 Kareish cheese and 50 yogurts] were collected from dairy shops, street venders and supermarkets from different localities in Alexandria Province to be investigated for presence of bacterial pathogens and to evaluate plasmid profiling as a tool for detection of bacteria transmitted through milk. The results of bacteriological examination firstly revealed that the rates of isolation of E. coli were 18, 26 and 18% from raw milk, kariesh cheese and yoghurt samples, respectively; secondly, results revealed that the rates of isolation of Salmonella species were 5, 6 and 2% from raw mill, kariesh cheese and yoghurt samples, respectively and finally they clarified that the rates of isolation of Staph. aureus were 44, 34 and 18% from raw milk, kariesh cheese and yoghurt samples, respectively. Serological identification of E. coli and Salmonella spp. was carried out. The public health significance of isolated bacteria and the diagnostic value of plasmid were discussed

Serological investigation of salmonellosis among some wild birds and human contacts

A total of 311 serum samples were collected from wild birds and their human contacts [211 from wild birds including gray heron, wild ducks, wild fowl, wild quail and wild captive birds and 100 from their human contacts]. All collected samples were subjected of serological response to Salmonella Typhimurium [ST] and Salmonella Entritidis [SE] by using the Widal and Felix test kits [ST antigen TMH code 63542] and [SE antigen ENH code 63272] supplied by Bio-Rad Laboratories. It was found that the overall percentage of Salmonella antibodies of wild birds was 13.7% [8.5 and 5.2% for ST and SE respectively]. On the other side, the overall seropositive percentage of human salmonellosis in examined serum samples of the human contacts was 10% and SE was most prevalent serotype affecting human contacts with wild birds. The effect of some epidemiological factors including sex and seasons of the year on the occurrence of salmonellosis in both wild birds and their human contacts were studied. The obtained results clarified that salmonellosis incidence was increased in males than in females with highest seasonal incidence occurring in winter season in both wild birds and human. The public health importance of Salmonellosis was discussed