ABSTRACT
Objective To investigate the correlation risk factors of diabetes over 16-year-olds at the community level in Chongqing and to set a quantitative criteria for determining risk for diabetes and to identify persons having potential risk.Methods 1981 cases with 1:2 matched controls were chosen from five communities in Chongqing including Shapingba,Xiaolongkan,Tianxingqiao,Yubei Road,Ciqikou,which were interviewed with a uniformed questionnaire.The risk factors of diabetes mellitus were analyzed with logistic regression,and to calculate the odds ratios of risk factors for diabetes.Different levels of risk exposure factors were converted into a risk scores,using statistical models.Results An individual health risk appraisal model of diabetes was established,applicable to individuals of different sex,age,health behavior,disease,and family history,for men,13 risk factors including hyperlipidemia(14.995),coronary heart disease(6.689),family history of hypertension(4.005),smoking(3.111)etc.while for women,hyperlipidemia(12.426),family history of hypertension(3.986),stroke(2.714),liking sweets(1.244),about 15 risk factors,were entering the main effect model.The related risk scores were added to obtain a combined risk score to predict the individual's risk of diabetes in the future.Conclusion The incidence of diabetes could be effectively reduced by changing the unhealthy lifestyle and curing the patient with the disease.Evidence was provided to persuade people change their unhealthy lifestyles and behaviors through health education.The results could also be used in community to improve their health services.
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the potential molecular mechanism responsible for the early time of tumor promotion, gene expression profile was studied in the transformed BALB/c 3T3 cells induced by 12-O-tetradecanoylphorbol-13-acetate (TPA).</p><p><b>METHODS</b>The two-stage cell transformation model was established by using the initiator of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and promoter of TPA. Cell proliferation was measured by trypan blue staining and cell cycle analysis was carried out by flow cytometry assay. A cDNA microarray representing 1 152 genes was used to investigate the gene expression profiles of BALB/c 3T3 cells exposed to TPA at 4 h and 24 h respectively.</p><p><b>RESULTS</b>TPA could effectively inhibit cell proliferation and induce the G1 and S cell cycle arrested in the early time. Moreover 19 genes were found differentially expressed at least twofold in the TPA treated cells as compared with the control cells, 9 of them were upregulated and 10 downregulated. Most of the differentially expressed genes were involved in cell proliferation, differentiation or apoptosis, and related to ras or p53 signal transduction pathway.</p><p><b>CONCLUSION</b>TPA could influence the transcriptional expression of some genes related to cell cycle modulation and ultimately result in the cell growth arrest.</p>