ABSTRACT
In order to discover the mechanism of Xuebijing oral effervescent tablet (XBJOET) to treat infectious diseases, the effect of XBJOET on endotoxin induced rabbit fever and disseminated intravascular coagulation (DIC) was investigated. Auricle microcirculation in rabbit was detected by laser speckle blood perfusion imager system; coagulation function was measured by coagulation analyzer, fibrinolytic system was quantified by Elisa assay and micro thrombosis in tissues was observed with HE staining under light microscope. The results demonstrated that the body temperature of rabbit decreased significantly at 1-3 h after administration with 4.8, 2.4 and 1.2 g x kg(-1) XBJOET to endotoxin induced DIC rabbit model, the auricle microcirculation blood flow in model group (54.45 +/- 14.53) PU was lower than that in control group (77.18 +/- 12.32) PU. The auricle microcirculation blood flow increased markedly and there was significant difference between model group and 1.2 g x kg(-1) XBJOET group. There was significant difference between model group and control group in the content of PAI1 and FIB. The PAI1 levels in model and control groups were (30.48 +/- 2.46) ng x mL(-1) and (20.93 +/- 3.25) ng x mL(-1), respectively. The FIB levels in model and control group were (3.34 +/- 1.09) g x L(-1) and (4.84 +/- 1.10) g x L(-1), respectively. The content of PAI1 in rabbit plasma decreased notably, there were significant differences between model group and 4.8, 2.4 g x kg(-1) XBJOET groups. On the contrary the content of FIB increased. XBJOET possessed pharmacological activities of curing infectious fever and DIC, the mechanism of which is related to amelioration of microcirculation disturbance, inhibition of fibrinolytic system activation and coagulation and micro thrombosis elimination.
Subject(s)
Animals , Female , Male , Rabbits , Administration, Oral , Blood Coagulation , Body Temperature , Disseminated Intravascular Coagulation , Blood , Drugs, Chinese Herbal , Pharmacology , Ear Auricle , Endotoxins , Fever , Drug Therapy , Fibrinogen , Metabolism , Microcirculation , Partial Thromboplastin Time , Plasminogen Activator Inhibitor 1 , Blood , Prothrombin Time , Tablets , Thrombosis , PathologyABSTRACT
This study is to investigate the treatment of Jin Chai antiviral capsule for influenza virus FM1/47 (H1N1) infection. The model of pneumonia was established by dropping influenza virus into the nose of normal mice, real-time PCR and Western blot technique were used to detect the virus load and the interferoninducible transmembrane protein3 (IFITM3) in lung of mice at the 1st day, 3rd day, 5th day and 7th day after affected. The results showed that Jin Chai antiviral capsule in large, middle, small dose groups can decrease virus load significantly at each time point, after being affected (P<0.05, P<0.01), Jin Chai antiviral capsule can increase the interferoninducible transmembrane protein3 in lung of mice, large dose groups are significantly higher in expression of IFITM3 compared with model group at each time point (P<0.05, P<0.01). Middle dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day and the 5th day (P<0.05), small dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day (P<0.05). It can be concluded that Jin Chai antiviral capsule exerts antiviral effects against influenzavirus by raised expression of IFITM3.
Subject(s)
Animals , Female , Male , Mice , Antiviral Agents , Pharmacology , Capsules , Dose-Response Relationship, Drug , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Influenza A Virus, H1N1 Subtype , Lung , Metabolism , Membrane Proteins , Metabolism , Mice, Inbred ICR , Orthomyxoviridae Infections , Metabolism , Virology , Plants, Medicinal , Chemistry , Pneumonia , Metabolism , Virology , Viral LoadABSTRACT
This study is to investigate the treatment of YinQiaojiedu soft capsule for influenza virus A/PR8/34 (H1N1) infection. The model of pneumonia was established by dropping influenza virus into the nose of normal mice, and the lung index and death rate were observed. Real time RT-PCR and Western blotting technique were used to detect the virus load and the relative expression of M1 protein in lungs of mice on the 1st, 3rd, 5th and 7th day after infection. The results showed that YinQiaojiedu soft capsule in 1 g x kg(-1) and 0.5 g x kg(-1) dose groups can decrease the lung index significantly on the 3rd, 5th and 7th day after being infected (P < 0.05, P < 0.01), and the number of death in the two groups of animals decreased significantly. YinQiaojiedu soft capsule in 1 g x kg(-1) dose group can decreased virus load at each time point, and lower it in 0.5 g x kg(-1) dose group at the 3rd, 5th and 7th day (P < 0.05, P < 0.01). YinQiaojiedu soft capsule can decrease the relative expression of M1 protein in lungs of mice, 1 g x kg(-1) and 0.5 g x kg(-1) dose groups are significantly lower in expression of M1 protein compared with model group at the 3rd and 7th day (P < 0.05, P < 0.01). It can be concluded that YinQiaojiedu soft capsule exerts antiviral effects against influenza virus by downregulating expression of virus load and M1 protein.
Subject(s)
Animals , Female , Male , Mice , Antiviral Agents , Pharmacology , Capsules , Drugs, Chinese Herbal , Pharmacology , Influenza A Virus, H1N1 Subtype , Lung , Metabolism , Virology , Mice, Inbred ICR , Orthomyxoviridae Infections , Drug Therapy , Metabolism , Virology , Pneumonia , Metabolism , Virology , Viral Load , Viral Matrix Proteins , MetabolismABSTRACT
It is to investigate the effect of two kinds of Houttuynia Cordata Injection on preventing and treating H1N1 influenza virus infection in mice. Pneumonia model was set up by intranasal infection of the normal and immunocompromised mice with influenza virus FM1 and PR8. The two injections were administered before and after the administration of virus, separately, and the lung index was observed. The results showed that the two preparations have obvious therapeutic effect on normal mice infected with influenza virus FM1 and PR8. And to FM1, the new injection's effect is better at small dosage. The results also showed that the two preparations have obvious prophylactic effect on immunodepressed mice infected with influenza virus FM1 and PR8. And to PR8, the old injection's effect is better at small dosage. Houttuynia Cordata Injection can improve the mice pneumonia caused by influenza virus H1N1 and decrease the lung index markedly. It has a remarkable preventive and therapeutic effect on H1N1 influenza virus in mice.
Subject(s)
Animals , Female , Male , Mice , Antiviral Agents , Therapeutic Uses , Drugs, Chinese Herbal , Therapeutic Uses , Houttuynia , Chemistry , Immunocompromised Host , Influenza A Virus, H1N1 Subtype , Allergy and Immunology , Injections , Mice, Inbred ICR , Orthomyxoviridae Infections , Drug Therapy , Allergy and Immunology , Plants, Medicinal , Chemistry , Pneumonia, Viral , Drug Therapy , Random AllocationABSTRACT
<p><b>OBJECTIVE</b>To explore the therapeutic effects and mechanisms of Zhubi capsule on adjuvant arthritis (AA) in rats.</p><p><b>METHOD</b>The model of rat was induced by intradermal injection of CFA. The perimeter of ankle joint was measured at different time points. Con A-induced splencyte proliferation was examined by MIT assay and the expression of IL-1, IL-6 in synovium was determined by immunohistochemical method.</p><p><b>RESULT</b>After the preventive administration of Zhubi capsule (5.2, 2.6, 1.3 g x kg x d(-1), x 25 d), it was found that Zhubi capsule significantly inhibited the primary and secondary ankle joint swelling. The increased Con A-induced splencyte proliferation reaction and the activated IL1, IL-6 expression of AA rat were suppressed by the treatment with Zhubi capsule.</p><p><b>CONCLUSION</b>Zhubi capsule has a therapeutic effect on AA rats, and its mechanism is related to the immunoregulative function.</p>
Subject(s)
Animals , Male , Rats , Ankle Joint , Pathology , Arthritis, Experimental , Metabolism , Pathology , Capsules , Cell Proliferation , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Interleukin-1 , Metabolism , Interleukin-6 , Metabolism , Materia Medica , Pharmacology , Membrane Fluidity , Plants, Medicinal , Chemistry , Rats, Wistar , Spleen , Cell Biology , Synovial MembraneABSTRACT
<p><b>OBJECTIVE</b>To compare the hemostatic effect of Flos Sophorae in crude, parched and carbonized forms and its extracts, including rutin, quercetin and tannin.</p><p><b>METHODS</b>All the testing samples were orally administered to the experimental animals for 5 days, then the bleeding time (BT), coagulation time (CT), platelet count and capillary permeability (CP) in the treated mice were tested, and the prothrombin time (PT), fibrinogen (FBG) content and platelet aggregation rate (PAR) in the treated rats were determined.</p><p><b>RESULTS</b>All the samples could lower the CP, BT and CT in mice and also decrease the plasma PT in rats. All three forms of Flos Sophorae could increase FBG in rats, while the three extracts of it could inhibit the PAR in rats obviously. In addition, rutin had the effect of raising the platelet count.</p><p><b>CONCLUSION</b>All the three forms and three extracts of Flos Sophorae have hemostatic effect, the effect of parched and carbonized form is higher than that of crude drug. The mechanism of the hemostatic effect of the six kinds of sample might be various.</p>