ABSTRACT
OBJECTIVE@#To investigate the expression of haptoglobin in the lesions of condyloma acuminatum (CA) at the mRNA and protein level, and to explore its role in the pathogenesis of CA.@*METHODS@#The expressions of haptoglobin protein and mRNA in the skin tissues of 30 patients with CA and 20 normal controls were detected by immunohistochemistry(IHC), Western blot, and hybridization in situ.@*RESULTS@#The in situ hybridization study showed that haptoglobin mRNA was expressed in the epidermal cells in the lesions of CA. The distribution of haptoglobin mRNA expression in the lesions of CA was similar to that of the normal controls, and the expression of haptoglobin mRNA in CA was higher than that of the normal controls. There was a significant difference in the positive expression of haptoglobin mRNA between the CA group and the control group (P<0.05). The immunohistochemical study showed that haptoglobin protein was expressed in the whole layers of epidermal keratinocytes in the lesions of CA at a high level and stronger staining was seen in the stratum basale and stratum spinosum. Haptoglobin protein was expressed predominantly in the stratum basale in normal skin tissues, while weak staining was seen below the stratum spinosum.There was a significant difference in the mean gray value between the CA group and control group (P<0.05). Western blot showed that the haptoglobin expression in CA lesions significantly increased compared with the normal skins (P<0.05).@*CONCLUSION@#The expression of haptoglobin mRNA in the CA lesions obviously increases and the epidermal cells in the CA lesions are able to synthesize haptoglobin protein. Haptoglobin in the CA lesions may involve in the local immunity escape by preventing Langerhans cell functional maturation and inhibiting the immunocompetence of keratinocyte.
Subject(s)
Adolescent , Adult , Aged , Humans , Male , Middle Aged , Young Adult , Blotting, Western , Case-Control Studies , Condylomata Acuminata , Genetics , Metabolism , Epidermal Cells , Haptoglobins , Genetics , Metabolism , In Situ Hybridization , Keratinocytes , Metabolism , RNA, Messenger , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To survey mycoplasma infection in female urogenital tract and analyze the drug sensitivity of mycoplasma in Changsha.</p><p><b>METHODS</b>Ureaplasma urealyticum (Uu) and Mycoplasma hominis (Mh) were detected in 6566 cases of female urogenital tract infection by means of mycoplasma culture and drug sensitivity reagent kit. Sensitivity tests for 10 antibiotics were also performed.</p><p><b>RESULTS</b>A total of 2938 cases were mycoplasma-positive (positivity rate of 44.75%), including 2469 Uu-positive cases (37.6%), 52 Mh-positive cases (0.08%) and 417 cases positive for both Uu and Mh (6.35%). Josamycin, doxycycline, clarithromycin and azithromycin were more effective against Uu infection. Josamycin, doxycycline and thiamphenicol were more effective against Mh infection. Mixed infection with Uu and Mh was more resistant to most antibiotics but Josamycin and doxycycline.</p><p><b>CONCLUSION</b>The female urogenital mycoplasma infection results mainly from Uu. Compared with simple Uu or Mh infection, mixed infection with Uu and Mh has significantly greater resistance to a wider variety of drugs. Josamycin and doxycycline are the primary choice for female urogenital mycoplasma infection in Changsha.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Middle Aged , China , Epidemiology , Doxycycline , Pharmacology , Therapeutic Uses , Drug Resistance, Bacterial , Josamycin , Pharmacology , Therapeutic Uses , Microbial Sensitivity Tests , Mycoplasma Infections , Drug Therapy , Epidemiology , Microbiology , Mycoplasma hominis , Ureaplasma Infections , Drug Therapy , Epidemiology , Microbiology , Ureaplasma urealyticum , Uterine Cervicitis , MicrobiologyABSTRACT
<p><b>OBJECTIVE</b>To observe the therapeutic effect of Xiaobai Mixture (XBM) in treating vitiligo.</p><p><b>METHODS</b>Seventy-four patients with vitiligo were randomly divided into the XBM group treated with XBM and the control group treated with 8-MOP. The therapeutic effect, nail-fold microcirculation, plasma endothelin-1, serum immunoglobulin were observed and compared.</p><p><b>RESULTS</b>The therapeutic effect of XBM was better than that of 8-MOP (P < 0.05). XBM could also obviously improve the nail-fold microcirculation, elevate the plasma endothelin-1 level and lower the serum IgG (P < 0.01).</p><p><b>CONCLUSION</b>XBM has superiority in treating vitiligo.</p>