In Silico Characterization of a Novel Bioactive Compound derived from Psidium guajava 4-[5-(Pyridin-4-yl)-1,2,4-oxadiazol-3-yl]-1,2,5-oxadiazol-3-amine: A Potential Inhibitor for Targeting Signaling Proteins involved in Diabetes Development

Pharmacological interventions for diabetes predominantly involve chemically synthesized compounds, often causing undesirable side effects. This has led to a growing interest in plant-based therapeutic alternatives. Technological advancements have facilitated the discovery of bioactive phytochemicals with medicinal properties. This study employs molecular docking analysis to assess the anti-diabetic potential of a naturally derived compound, 4-[5-(Pyridin-4-yl)-1,2,4-oxadiazol-3-yl]-1,2,5-oxadiazol-3-amine (POA), obtained from Psidium guajava leaf extract. The evaluation focuses on its inhibitory action against four human proteins 11 ? -HSD1 (PDB: 4K1L), GFAT (PDB ID: 2ZJ4), SIRT6 (PDB ID: 3K35) and Aldose reductase (PDB ID: 3G5E) associated with diabetes. Physicochemical, pharmacokinetic, and ADMET profiles were computed using online web servers Molinspiration, ADMETLAB 2.0, and SWISSADME. POA demonstrated superior binding affinity (in Kcal/mol) -8.0, -7.5, -8.9 and -9.5, respectively) compared to the widely used diabetic drug Metformin -5.4, -6.0, -5.4 and -7.2 with these receptor proteins. Based on molecular docking studies and pharmacokinetics/ADMET profiles, POA may act as a multi-targeted, less harmful, and more efficacious medication for Type 2 Diabetes Mellitus (T2DM) compared to Metformin.

Effect of Different Liquid Biofertilizers and Varying Fertility Levels on Available N, P2O5, K2O and S Content after Harvesting in Soil

The experimental trail was conducted at the farm of Rajasthan Agricultural Research Institute (RARI), Durgapura, Jaipur which is the sub campus of Sri Karan Narendra Agriculture University, Jobner, Jaipur (Raj.). In the research study, observed the effect of different liquid biofertilizers and fertility levels on growth, yield and quality of cluster bean (cymopsis tetragonoloba). The test crops were grown on field during July to November kharif-2018 and 2019. The experiment was raised in factorial randomized block design (FRBD). The treatments of experiment comprised of two factors (1) three treatment of fertility levels: 100 % recommended dose of fertilizers (F1), 75% RDF (F2) and 50 % RDF (F3) and eight treatment of liquid biofertilizer : Control-no biofertilizers (L0), Rhizobium (L1), PSB-phosphorus solubilizing bacteria (L2), KMB-potassium solubilizing bacteria (L 3), SSB-sulphur solubilizing bacteria (L 4) , Rhizobium + PSB (L 5 ), Rhizobium + PSB+ KMB (L 6 ) and Rhizobium + PSB+ KMB + SSB (L 7). Total number of treatments were 24 and replicated three times. The result observed that highest nutrient status found in the combination of 100 % RDF (F1) with Rhizobium + PSB+ KMB + SSB (L 7).