ABSTRACT
Introduction: Tumors like Odontogenic Keratocyst (OKC), Dentigerous Cyst (DC)and Pyogenic Granuloma are frequently oc- curring in the oral cavity with each of them having relation to angiogenesis. Higher angiogenesis may be associated with increased tissue metabolism, more aggressive biologic behaviour, and increased recurrence and growth rate. Tumor growth is dependent not only on a rise in the number of blood vessels, but also on factors such as protein molecules produced in en- dothelial cells. Microvessel density (MVD), Microvessel area (MVA), Microvessel perimeter (MVP) can predict the growth of the tumour, metastasis and patient’s survival and this value is related to the aggressiveness of the tumour. Aims: The aim of the present study was to determine the angiogenic potential of OKC and DCcompared with normal mucosa using CD 105 marker immunohistochemically. Materials and methods: Immunohistochemical staining was done on 70 paraffin embedded tissue samples. Histopathologi- cally diagnosed cases of OKC, DC and Pyogenic granuloma and healthy gingival tissue samples were retrieved for the study purpose. Results: There was no statistically significant difference in the mean MVD, MVA, MVP values of OKC, DC and pyogenic granu- loma groups. Conclusion: The angiogenic potential was determined in 3 different cases of OKC, Dentigerous Cyst and Pyogenic granuloma in terms of MVD, MVA and MVP and compared to normal mucosa using CD105 marker immunohistochemically.Though the mean values of MVA, MVD, MVP were statistically not significant but was estimated to be higher than the normal mucosa
ABSTRACT
Context: Due to limited resources and/or affordability by majority of the patients, many centers in low- and middle-income countries are still not able to adapt three-dimensional image-based brachytherapy planning in their routine practice. Aim: The aim of the study was to see the feasibility of using computed tomography (CT)-based plan of the first fraction to treat successive fractions of intracavitary brachytherapy based on the estimation of the physical dosimetric differences between successive applications. Materials and Methods: CT image-based brachytherapy plans of 38 patients who received three insertions of intracavitary application with high-dose-rate brachytherapy have been analyzed. Revised plans for the second and third insertions were generated by adapting dwell time and dwell position of the first insertion plan. The dose to point “A” and maximum doses to 2, 1, and 0.1 cc volumes of the rectum and bladder have been used for dosimetric comparison. Results: The statistical differences of mean point “A” doses were observed insignificant except between original and revised plans for the second insertions. The dosimetric differences between consecutive original and revised plans for the bladder and rectum have not shown any significance except minimum dose to 0.1 cc volume of the rectum for the third insertions. Conclusions: Dosimetric deviation for tumor and organs at risk is within acceptable limit while using CT image-based brachytherapy plan of the first fraction for treating successive fractions
ABSTRACT
Ground sections of teeth or bone are considered best solutions for the study of any hard tissues in the study of histopathology. Various studies have been performed like progression of dental caries, enamel studies, dentin studies, cementum annulation studies, bone morphologies, age determinaton studies, etc. It is important study part in any forensic studies where mass disasters occurring as natural phenomenon of cyclonic events, earth quakes, floods and volcanic explosions and airplane accidents, industrial accidents and terror related acts. Forensic dentistry has played a vital role and many medico-legal cases are solved by studying the teeth. Ground sections study is important in any part of research. For a dentist this phenomenon is less understood, and this article brings in limelight various methods and study pattern of ground sections of teeth. With this knowledge an understanding and performing ground sections of teeth becomes a well start for dental graduates. Various other modalities in histopathology are decalcification studies, histopathological staining procedures, special stains procedures, immunohistochemistry. All the histopathological modalities have their own importance and are used appropriately in the study of oral pathology.