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OBJECTIVE To establish the fingerp rint of decoction pi eces and dispensing granules of Gardenia jasminoides ，to determine the contents of 6 components，so as to evaluate its quality combined with chemical pattern recognition. METHODS High performance liquid chromatography （HPLC）was used. Using geniposide as the reference ，Similarity Evaluation System for Chromatographic Fingerprint of TCM （2012 edition）was used to draw the fingerprints of 20 batches of G. jasminoides decoction pieces and 10 batches of G. jasminoides dispensing granules. Similarity evaluation and common peaks identification were conducted. The same HPLC method was adopted to determine the contents of deacetyl asperulosidic acid methyl ester ，geniposide， picrocrocin，rutin，crocin-Ⅰ and crocin- Ⅱ. ORIGIN 9.1 software was used for hierarchical clustering analysis ，and SIMCA 16.0 software was used for principal component analysis （PCA） and partial least squares-discriminant analysis. The differential components affecting the quality of decoction pieces and dispensing granules were screened by taking the variable importance in projection（VIP）value＞1 as the standard. RESULTS There were 24 common peaks for both 20 batches of G. jasminoides decoction piece and 10 batches of G. jasminoides dispensing granules ；a total of 22 common peaks were found in the fingerprints of 30 batches of samples ，and the similarity was not lower than 0.96；six common peaks were identified ，i.e. deacetyl asperulosidic acid methyl ester （peak 2），geniposide（peak 6），picrocrocin（peak 9），rutin（peak 11），crocin-Ⅰ（peak 15），crocin-Ⅱ（peak 17）. Average contents of above 6 components in G. jasminoides decoction pieces were 1.04，57.00，1.30，1.03，9.63 and 0.99 mg/g， respectively；those of G. jasmin oides dispensing granules were 0.96，17.04，0.37，0.27，0.73 and 0.04 mg/g，respectively. PCA results showed that G. jasminoides decoction pieces and G. jasminoides dispensing granules were clustered into respective one category ，which was consistent with results of cluster analysis. There were 9 common peaks with VIP value ＞1， which were 16，14，3，17（crocin-Ⅱ），15（crocin-Ⅰ），18， 22， 2 （deacetyl asperulosidic acid methyl ester） and 21. CONCLUSIONS The estab lished fingerprint and content determination method are simple and reproducible. Combined with chemical pattern recognition ，it can be used to evaluate the quality of decoction pieces and dispensing granules of G. jasminoides . Nine corresponding components represented by peak 16 and so on are the differential components that affect the quality of them.
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s: Cyclocarya paliurus (Batal.) Iljinsk. polysaccharide (CPPC) has various biological activities including hypolipemic effect, hypoglycemic effect, anti-oxidant effect, anti-tumor effect and immunomodulatiry;especially that hypolipemic effect and hypoglycemic effect have become the research hotspots in recent years. This article reviewed extraction, purification, content determination and biological activity of CPPC in the latest decade, with a purpose to provide references for the deep research and industrial development CPPC.
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Objective To simultaneously determine the quantify liquiritin, glycyrrhetinic acid and glycyrrhizic acid inXianlong-Jiedu Yin by high performance capillary electrophoresis (HPCE).Methods HPCE method was performed on a fused silica capillary coates 75μm×85 cm (effective column length 78.5 cm) using 50 mmol/L borax solution-acetonitrile (95:5), with adjusted pH=8.50 as running buffer, lytical 22 kV, and electrokinetic injection 10 s, detected wave length of 254 nm, and temprature maintaining 25℃.Results The liquiritin, glycyrrhetinic acid and glycyrrhizic acid were completely separated within 30 minutes, showing good linear relationship in the range of 12-120, 15-150, 8-160μg/ml (r>0.999 3). The average recoveries were 100.70%, 98.51%, 98.40% withRSD 2.49%, 2.79%, 0.40%, respectively.Conclusions The HPCE was suitable for the determination of liquiritin, glycyrrhetinic acid and glycyrrhizic acid inXianLong-JieDu Yin, which can be used for the quality control.
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Objective:To optimize the extraction technology for Sanren Tongbian mixture. Methods:The water amount,decoction time and decoction frequency as the evaluation factors,and the content of amygdalin and the paste-forming rate as the evaluation indi-ces,the extraction process of Sanren Tongbian mixture was optimized by L9 (34 ) orthogonal design. Results: The optimal extraction conditions were as follows:adding 8-fold amount of water, decocting twice with 1. 5h for each time. Conclusion: The study provides scientific basis for the research of the extraction technology of Sanren Tongbian mixture, and the further experiments proved that the op-timized process is stable and feasible.
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OBJECTIVE:To study antioxidant activities of ethanol extract of Paeonia lactiflora (CREt) and different polar parts in vitro. METHODS:CREt was prepared with 95% ethanol. CREt (extracted by 50% ethanal) was extracted with aether petrolei,acetic ether and n-butyl alcohol to obtain aether petrolei part (CRP part),acetic ether part (CRE part),n-butyl alcohol part(CRB part)and water part(CRW part). The ability of CREt and different polar parts eliminating DPPH·,O2-· and ·OH were investigated (mass concentrations of CREt and different polar parts were respectively 0.75-12,0.5-6,1.25-15 mg/ml in above 3 tests) and compared with ascorbic acid (VC,0.2 mg/ml) group. RESULTS:The maximum elimination rate of CREt to DPPH·, ·OH and O2-· were(97.55±0.25)%,(81.45±0.20)% and(75.28±0.41)%,IC50 were 1.629,1.789 and 5.268 mg/ml;those of CRE part to those radicals were (82.54 ± 0.36)%,(77.74 ± 0.42)% and (72.16 ± 0.73)%,IC50 were 2.481,1.918 and 6.005 mg/ml;that of CRB part to ·OH reached to(62.53±0.83)%,IC50 was 7.232 mg/ml,but to DPPH·、O2-· were less than 45%;those of CRP part and CRW part to those radicals were all lower than 40%. Each part could eliminate 3 radicals in dose-dependent manner,but were all poorer than VC group,with statistical significance (P<0.01). CONCLUSIONS:The CREt and CRE part show strongest antioxidant activities in vitro,and other parts have weak antioxidant effect.