ABSTRACT
Objective To compare the variation of ferulic acid contents in Naomaitong by different extraction processes.Methods With ethanol or water as extracting solvent,the four herbal medicines of Rhizoma Chuanxiong,Radix Ginseng,Radix Puerariae,Radix et Rhizoma Rhei,which consisted of Naomaitong prescription,were decocted together or decocted separately firstly then mixed together were evaluated.With ferulic acid extraction rate as the index,the above extraction processes were evaluated.Results The extraction rate of ferulic acid extracted by alcohol was higher than that by water,but the ferulic acid content showed no obvious difference by decocting together or decocted separately firstly then mixed together.Conclusion It is suggested that proper extraction solvents and extraction methods should be adopted according to the different physicochemical characteristics of chemical contents in herbal medicine during extraction.
ABSTRACT
AIM To study effect of 3,4 oxo isopropylidene shikimic acid(ISA) on free radical metabolism in the brain issue of rats subjected to middle cerebral artery thrombosis.METHODS Adopt a middle cerebral artery thrombosis model to determine the activity of T SOD, Cu Zn SOD, MDA and GSH px of brain issue of rats by the way of colorimetry.RESULTS ISA 100 and 200 mg?kg -1 ig for 2 times in 24 hours raised the activity of T SOD;ISA 50, 100 and 200 mg?kg -1 ig,increased the activity of Cu Zn SOD and GSH px in rats brain issue ;ISA 50, 100 and 200 mg?kg -1 ig for 2 times in 24 hours,decreased the content of MDA in rats brain issue. CONCLUSIONS ISA protect brain issue from the ischemic damage by modifying the free radical metabolism.