ABSTRACT
Objective To investigate the electrophysiological characteristics of neurons in developing visual cortex and to observe the reciprocal connection and the function of integration between adjacent neurons. Methods Whole cell recordings of the visual cortex from SD rats (4～28 d old) were obtained by using "blind" whole cell patch clamp recording technique. Bipolar stimulating electrodes were placed in the white border under the visual cortex. Postsynaptic currents(PSCs) were evoked by using stimulation of 0 1 Hz. Results There were three types of PSCs recorded in 156 cells in the first developmental month: irresponse, monosynaptic response and polysynaptic response. The incidence of polysynaptic PSCs increased from 12 36% before eye opening to 31 34% after eye opening ( P
ABSTRACT
Objective To investigate the relationship between electrophysiological and morphological properties of neurons in visual cortex of developing rat, speculate the coincided degree between electrophysiological and morphological change and realize the mechanism of normal visual development. Methods Whole cell patch-clamp recording and intracellular staining were used to acquire cellular microelectrode recording in visual cortex from Sprague-Dawley rats (4~28 days old). The histological process was made. Results The differences of electrical feature between pyramidal cells and non-pyramidal cells were significant. The morphological maturity degree is different in developing visual cortex. Conclusion The different function of pyramidal and non- pyramidal cells in local integrition is reflected by their electrical feature in the process of visual development. In critical period of visual development, the coincision degree of the electrophysiological and morphological change in visual cortex is larger than that in the subcortex constructure.