ABSTRACT
OBJECTIVE： To establish the method for content determination of volatile oil of Schizonepeta tenuifolia and Forsythia suspensa， and to optimize the extraction technology of the volatile oil. METHODS： The contents of β-pinene and pulegone were determined by GC method. The determination was performed on Hp-5 capillary column. The detector was hydrogen flame ion detector with programmed temperature. The sample size was 0.5 μL， the split ratio was 70 ∶ 1， the carrier gas was nitrogen， the inlet temperature was 250 ℃， the detector temperature was 280 ℃， the air flow rate was 390 mL/min， the hydrogen flow rate was 36 mL/min， the tail flow rate was 15 mL/min， and the nitrogen flow rate was 1 mL/min. Based on single factor test， orthogonal test combined with information entropy method were used to optimize the extraction technology of S. tenuifolia and F. suspensa using soaking time， extraction time， material-liquid ratio and forsythia grain size as factors， with the extraction amount of volatile oil， the content of β-pinene and pulegone and their comprehensive score as indexes. RESULTS： The linear range of β-pinene and pulegone 1.575-7.875（r＝0.999 9） and 1.892-9.46 μg（r＝0.999 7）， respectively. The limits of quantitation were 0.10 and 0.25 μg； the limits of detection were 0.03 and 0.08 μg； RSDs of precision， stability and reproducibility tests were less than 2％ （n＝6）； the recoveries were 97.77％-100.01％ （RSD＝0.93％，n＝9） and 96.47％-99.00％（RSD＝0.89％， n＝9）. The optimal extraction technology was soaking 2 h， extracting for 6 h， 10-fold water （mL/g）， half a clove of granularity. Under this condition， the extraction amount of volatile oil， the contents of β-pinene and pulegone were 3.6 mL， 1 450.4 mg， 127.6 mg， respectively. RSD were 1.62％， 0.20％， 1.42％. CONCLUSIONS： Established method is simple， accurate and reproducible， and the optimal extraction technology is stable and feasible.
ABSTRACT
Objective To observe the effect of simvastatin on serum levels of matrix metalloproteinase-9(MMP-9) and tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) in patients with acute coronary syndromes (ACS).Methods 74 patients with coronary heart disease were studied.Among them,there were 34 patients with stable angina pectoris(SAP) and 40 patients with ACS.At the same time 25 cases of age matched healthy individuals were selected as a control.The serum level of MMP-gand TIMP-1 were compared among all groups.Results There was a significant difference in serum level of MMP-9and TIMP-1 among SAP group,ACS group and control group.The serum level of MMP-9 and TIMP-1 were different after treatment.Conclusion Plaque disruption may be related to the increased serum level of MMP-9 and the decreased serum level of TIMP-1 .Simvastatin can decrease serum level of MMP-9 and increase serum level of TIMP-1 in patients with ACS,and it may have a beneficial effect on clinical treating of patients with ACS.
ABSTRACT
0.05).Improvement in the total effective rate of motor function was significant in the combination group compared with Bonin group or internal irradiation group (P