ABSTRACT
Objective To investigate the clinical features,treatment regimen and prognosis of acute lymphoblastie leukemia (ALL) complicating central nervous system leukemia (CNSL).Methods The clinical data,results of laboratory detection and imageological examination in the patients with ALL complicating CNSL in this hospital from January 2005 to January 2015 were retrospectively analyzed.Results A total of 83 cases were collected,including 52 male cases(62.65％) and 31 female cases (37.35％) with a median age of 27 (15-72) years old.Among them,19 cases were T cells ALL(T-ALL),54 eases were B cell ALL(B-ALL),3 cases were Burkitt lymphoma/leukemia,2 cases were ALL (T/B lymphocyte) and 5 cases were undefined type.The median follow-up time was 10(1-52) months.The univariate analysis results showed that the patients with high WBC count (WBC≥100× 109) at initial diagnosis or unreach complete remission (CR) after 1 treatment course had relatively poor prognosis.Receiving the radiotherapy could improve the patients' survival rate(P＜0.05).In the multivariate analysis,high WBC count at initial diagnosis was an independent prognostic factor affecting the patients' overall survival(OS) (P=0.007).Conclusion The clinical features in the patients' with ALL complicating CNSL have poor specificity,high WBC level and unreaching CR by 1 treatment course are the poor prognosis factors,and local radiotherapy has a certain significance for prolonging the patients' survival period.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of polypeptide K237 on the proliferation of human hormone refractory prostate cancer cell line PC-3M and its possible mechanism.</p><p><b>METHODS</b>PC-3M cells were divided into three experimental groups and a control, treated with polypeptide K237 at the concentration of 50, 100, 200 and 0 micromol/L, respectively, for 48 hours. The effects of K237 on the proliferation of different groups of the PC-3M cells were analyzed by MTF, and the mRNA expression levels of bax and bcl-2 were detected by RT-PCR.</p><p><b>RESULTS</b>After polypeptide K237 treatment, the PC-3M cells became round, small and less transparent in cytoplasm, and some shed and suspended in the culture medium. The growth inhibition rates of the PC-3M cells were (12.6 +/- 0.95)%, (17.8 +/- 0.99)% and (27.2 +/- 1.12)% in the 50, 100 and 200 micromol/L concentration groups. RT-PCR analysis showed that the bax/beta-actin values of the 50, 100, 200 and 0 micromol/L groups were 0.919 +/- 0.071, 0.971 +/- 0.083, 0.992 +/- 0.102 and 0.889 +/- 0.067, and the bcl-2/beta-actin values of the four groups were 0.896 +/- 0.085, 0.791 +/- 0.084, 0.764 +/- 0.702 and 0.922 +/- 0.097, respectively, both with significant differences between the experimental and the control groups (P < 0.01). The mRNA expression of bax was upregulated and that of bcl-2 downregulated in a dose-dependent manner.</p><p><b>CONCLUSION</b>Polypeptide K237 may induce apoptosis of PC-3M cells by affecting the expressions of bax and bcl-2, and thus suppress the proliferation of prostate cancer cells.</p>